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Methods and analysis


  • 2-D Electrophoresis – determination of isoelectric point and molecular mass, analysis of protein profiles;
  • Isolation of protein and peptides using FPLC, determination of the main physico-chemical properties of protein;
  • Measurement of sperm concentration;
  • Kinetics analysis (determination of enzyme and inhibitor concentration, measurement of the equilibrium association constant for inhibitor – proteinase complexes;
  • Electroelution of proteins from polyacrylamide gel.