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Methods and analysis


  1. Flow cytometry
    • estimation of cells proliferation (cell growth cycle analysis of different cells from in vitro cultures)
    • analysis of cell apoptosis (anexin V)
    • sorting of labeled cells or molecules
  2. Confocal microscopy
    • high resolution imaging of objects in 3D (optional with time-lapse imaging)
  3. Microdissection – MMI system
    • contamination free isolation of selected biological material
    • isolation of single cells or tissue fragments from fixed specimens
    • isolation of cells or cell clones from intravital cultures
  4. Cells cultures
    • cell clones isolation
    • cell lines immortalization
      • lipotransfection
      • electroporation
    • vector transfection
    • preservation in liquid nitrogen