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Equipment and applied methods P&ToR

Preparation of the material for the studies:

  • The isolation of cells of corpus luteum, myometrium, endometrium and epithelium of oviduct
  • The cell cultures and strip incubation: an incubator (Memmert, Germany)

Analytical methods:

  • Viability of cells determination (cytotoxicity test): ELISA (Multiscan EX, Labsystems, Finland)
  • Enzyme immunoassay of steroids, protein hormones and prostaglandins (ELISA, Multiscan EX, Labsystems, Finland)
  • PCR and its modifications: RT-PCR, nested-PCR, touchdown PCR
  • RT PCR and Real-time PCR:
    • RNA isolation (spectrophotometer BioPhotometr, Eppendorf, and Nonodrop)
    • Reverse transcription, PCR (termocycler MJ Mini, BioRad)
    • Real-time PCR (access to: ABI Prism 7300, Applied Biosystems, USA)
    • Gel documentation system for electrophoresis gels (MiniBIS Pro, DNR Bio Imaging System, Israel)
  • 5’ and 3’ RACE PCR – Rapid Amplification of cDNA Ends PCR
  • Western Blot analysis (Mini PROTEAN 3 System)
  • Intracellular Ca2+ mobilization using Fura 2 (microscope Nikon Eclipse TS 100 NIS-Elements Basic Research software)
  • Immunohistochemistry (access to: microscope Imager Z1-Zeiss, Axio Vision 4.8 software)
  • Measurement of contractility of myometrial and oviductal strips: isometric contraction transducer (access to: the HSE Type 372, Hugo Sachs Elektronik, Germany)
  • Determination of catecholamine concentrations by HPLC with electrochemical detection (Hewlett-Packard, Series 1050)

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Publications P&ToR

2025

  1. Jana, Barbara; Andronowska, Aneta; Całka, Jarosław; Mówińska, Aleksandra. Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cells. Scientific Reports 2025, 15: art. no 2806 (1-11) (10.1038/s41598-025-86787-1).

  2. Mówińska, Aleksandra; Rękawiecki, Robert; Zając, Paulina; Kowalik, Magdalena K. Phoenixin-14 and its receptor GPR173 in the bovine endometrium: Expression and regulation during the estrous cycle and impact on secretory activity. Theriogenology 2025, 243: art. no 117456 (1-8) (10.1016/j.theriogenology.2025.117456).

  3. Sienkiewicz-Szłapka, Edyta; Janysz, Arleta; Wróbel, Michał H. Quantification of lawsone and p-phenylenediamine in natural henna products for hair colouring available in poland. Contact Dermatitis 2025, 92(4): 261-272 (10.1111/cod.14739).

2024

  1. Herman, Andrzej P.; Tomczyk, Monika; Wójcik, Maciej; Bochenek, Joanna; Antushevich, Hanna; Herman, Anna; Wiechetek, Wiktoria; Szczepkowska, Aleksandra; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota. Effect of caffeine on the inflammatory-dependent changes in the GnRH/LH secretion in a female sheep model. International Journal of Molecular Sciences 2024, 25(5): art. no 2663 (1-15) (10.3390/ijms25052663).

  2. Neuman, Nicoletta M.; Dziekońska, Anna; Orzołek, Aleksandra; Gilun, Przemysław D. A comparison of the biological properties of European red deer (Cervus elaphus elaphus) spermatozoa stored in the epididymides and in a liquid state at 5 °C. Animal Reproduction Science 2024, 260: art. no 107387 (1-13) (10.1016/j.anireprosci.2023.107387).

  3. Piotrowska-Tomala, Katarzyna K.; Szóstek-Mioduchowska, Anna Z.; Jończyk, Agnieszka W.; Drzewiecka, Ewa M.; Wróbel, Michał H.; Hojo, Takuo; Ferreira-Dias, Graca; Skarżyński, Dariusz J. The effect of lysophosphatidic acid on myometrial contractility and the mRNA transcription of its receptors in the myometrium at different stages of endometrosis in mares. BMC Veterinary Research 2024, 20: art. no 571 (1-14) (10.1186/s12917-024-04384-2).

  4. Rybska,Marta; Skrzypski, Marek; Billert, Maria; Wojciechowicz, Tatiana; Łukomska, Anna; Pawlak, Piotr; Nowak, Tomasz; Pusiak, Karolina; Wąsowska, Barbara. Nesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14. BMC Veterinary Research 2024, 20: art. no 486 (1-11) (10.1186/s12917-024-04336-w).

  5. Wojciechowska, Anna M.; Zając, Paulina; Gogola-Mruk, Justyna; Kowalik, Magdalena K.; Ptak, Anna . Myo-inositol and D-chiro-inositol reduce DHT-stimulated changes in the steroidogenic activity of adult granulosa cell tumors. International Journal of Molecular Sciences 2024, 25(20): art. no 10974 (1-12) (10.3390/ijms252010974).

  6. Wróbel, Michał H.; Młynarczuk, Jarosław; Rękawiecki, Robert. Effects of commonly used carbamates (carbaryl and thiram) on the regulatory, secretory and motor functions of bovine cervixes in vitro. Theriogenology 2024, 218: 183-192 (10.1016/j.theriogenology.2024.01.030).

2023

  1. Fabová, Zuzana; Tarko, Adam; Mlynček, Miloš; Kotwica, Jan; Sirotkin, Aleksandrer V. Flaxseed can Prevent Adverse Effects of Toluene on Human Ovarian Cell Functions. Current Bioactive Compounds 2023, 19(7): art. no e020123212322 (1-xx) (10.2174/1573407219666230102121435).

  2. Gilun, Przemysław; Koziorowska-Gilun, Magdalena; Wąsowska, Barbara; Sowa-Kućma, Magdalena; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Kordan, Władysław; Koziorowski, Marek. Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season. Polish Journal of Veterinary Sciences 2023, 26(1): 65-82 (10.24425/pjvs.2023.145008).

  3. Rękawiecki, Robert; Wróbel, Michał H.; Zając, Paulina; Serej, Oliwia; Kowalik, Magdalena K. Luteotropic and luteolytic factors modulate the expression of nuclear receptor coregulators in bovine luteal cells independently of histone acetyltransferase and histone deacetylase activities. Animals 2023, 13(17): art. no 2784 (1-16) (10.3390/ani13172784).

  4. Rybska, Marta; Billert, Maria; Skrzypski, Marek; Wojciechowicz, Tatiana; Kubiak, Magdalena; Łukomska, Anna; Nowak, Tomasz; Włodarek, Jan; Wąsowska, Barbara. Expression and localization of the neuropeptide phoenixin-14 and its receptor GRP173 in the canine reproductive organs and periovarian adipose tissue. Animal Reproduction Science 2023, 255: art. no 107282 (1-18) (10.1016/j.anireprosci.2023.107282).

  5. Sirotkin, Alexander V.; Macejková, Martina; Tarko, Adam; Fabova, Zuzana; Alwasel, Saleh; Kotwica, Jan; Harrath, Abdel H. Ginkgo, fennel, and flaxseed can affect hormone release by porcine ovarian cells and modulate the effect of toluene. Reproductive Biology 2023, 23(1): art. no 100736 (1-6) (10.1016/j.repbio.2023.100736).

  6. Sirotkin, Alexander V.; Tarko, Adam; Fabova, Zuzanna; Valocky, Igor; Alwasel, Saleh; Kotwica, Jan; Harrath, Abdel H. Can flaxseed, chia or puncture vine affect mare ovarian cell functions and prevent the toxic effect of the environmental contaminant toluene. Theriogenology 2023, 208: 178-184 (10.1016/j.theriogenology.2023.06.018).

  7. Sirotkin, Alexander V.; Tarko, Adam; Kotwica, Jan; Mlyncek, Milos; Alwasel, Saleh; Nitrayova, Sona; Harrath, Abdel H. Three medicinal plants affecting human ovarian cell viability, hormone release, and response to environmental contaminant toluene. Physiological Reports 2023, 72(2): 259-268 (10.33549/physiolres.934986).

  8. Szczepkowska, Aleksandra; Bochenek, Joanna; Wójcik, Maciej; Tomaszewska-Zaremba, Dorota; Antushevich, Hanna; Tomczyk, M.; Skipor, Janina; Herman, Andrzej P. Effect of caffeine on adenosine and ryanodine receptor gene expression in the hypothalamus, pituitary, and choroid plexus in ewes under basal and LPS challenge conditions. Journal of Animal and Feed Sciences 2023, 32(1): 17-25 (10.22358/jafs/156762/2022).

  9. Tarko, Adam; Štochmaľová, Aneta; Harrath, Abdel H.; Kotwica, Jan; Baláži, Andrej; Sirotkin, Alexander V. Quercetin can affect porcine ovarian cell functions and to mitigate some of the effects of the environmental contaminant toluene. Research in Veterinary Science 2023, 154: 89-96 (10.1016/j.rvsc.2022.12.005).

2022

  1. Dobrzyń, Karolina; Kowalik, Magdalena K. Molekularny mechanizm działania progesteronu w żeńskim układzie rozrodczym [Molecular mechanism of progesterone action in the female reproductive system]. Postępy Biochemii – Advances in Biochemistry 2022, 68(3): 336-343 (10.18388/pb.2021_458).

  2. Fabová, Zuzana; Tarko, Adam; Harrath, Abdel Halim; Alwasel, Saleh; Kotwica, Jan; Sirotkin, Alexander V. Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells. Reproduction in Domestic Animals 2022, 57(11): 1307-1318 (10.1111/rda.14204).

  3. Kowalik, Magdalena K.; Dobrzyń, Karolina; Młynarczuk, Jarosław; Rękawiecki, Robert. Effect of steroid hormones, prostaglandins (E2 and F2α), oxytocin, and tumor necrosis factor alpha on membrane progesterone (P4) receptors gene expression in bovine myometrial cells. Animals 2022, 12(4): art. no 519 (1-13) (10.3390/ani12040519).

  4. Mańkowska, Anna; Gilun, Przemysław D.; Zasiadczyk, Łukasz; Sobiech, Przemysław; Fraser, Leyland. Expression of TXNRD1, HSPA4L and ATP1B1 genes associated with the freezability of boar sperm. International Journal of Molecular Sciences 2022, 23(16): art. no 6320 (1-18) (10.3390/ijms23169320).

  5. Nowak, Sławomir; Gilun, Przemysław; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Koziorowska-Gilun, Magdalena; Wąsowska, Barbara. Carbon monoxide (CO) as a retinal regulator of heme oxygenases -1, and -2 (HO’s) expression. Biomedicines 2022, 10(2): art. no 358 (1-9) (10.3390/biomedicines10020358).

  6. Rybska, Marta; Billert, Maria; Skrzypski, Marek; Kubiak, Magdalena; Woźna-Wysocka, Magdalena; Łukomska, Anna; Nowak, Tomasz; Błaszczyk-Cichoszewska, Joanna; Pomorska-Móla, Małgorzata; Wąsowska, Barbara. Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression. Animal Reproduction Science 2022, 238: art. no 106931 (1-13) (10.1016/j.anireprosci.2022.106931).

  7. Sirotkin, Alexander V.; Tarko, Adam; Fabova, Zuzana; Mlynček, Miloš; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. Direct action of leptin, obestatin and ginkgo on hormone release by luteinised human ovarian granulosa cells [Erratum: Reproduction, Fertility and Development 2022, 34(18): 1128-1146, doi: 10.1071/RD22154_CO]. Reproduction, Fertility and Development 2022, 34(18): 1128-1134 (10.1071/RD22154).

  8. Wróbel, Michał H.; Młynarczuk, Jarosław; Kowalik, Magdalena K. Do cypermethrin and fenvalerate disturb the function of the bovine cervix in vitro. Journal of Endocrinology 2022, 253(3): 133-142 (10.1530/JOE-21-0336).

  9. Wróbel, Michał H.; Młynarczuk, Jarosław; Rękawiecki, Robert. Do commonly used herbicides (atrazine and glyphosate) have the potential to impair the contractions, prostaglandin releasing and conducting of oxytocin signal at the bovine cervix in vitro. Theriogenology 2022, 183: 26-35 (10.1016/j.theriogenology.2022.02.011).

2021

  1. Domżalska, Małgorzata; Wiczkowski, Wiesław; Szczepkowska, Aleksandra; Chojnowska, Sylwia; Misztal, Tomasz; Walter, Fruzsina R.; Deli, Maria A.; Ishikawa, Hiroshi; Schroten, Horst; Schwerk, Christian; Skipor, Janina. Effect of lipopolysaccharide-induced inflammatory challenge on β-glucuronidase activity and quercetin and its metabolites concentration in the choroid plexus, blood plasma and cerebrospinal fluid. International Journal of Molecular Sciences 2021, 22(13): art. no 7122 (1-17) (10.3390/ijms22137122).

  2. Gilun, Przemysław D.; Flisikowski, Krzysztof; Flisikowska, Tatiana; Kwiatkowska, Joanna; Wąsowska, Barbara; Koziorowska-Gilun, Magdalena. Role of methylation in period2 (PER2) transcription in the context of the presence or absence of light signals: natural and chemical – studies on the pig model. International Journal of Molecular Sciences 2021, 22(15): art. no 7796 (1-17) (10.3390/ijms22157796).

  3. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Dobrzyń, Karolina. The effect of DDE and dieldrin and their parental substances (DDT and aldrin) on PGE2 and PGF2α release from bovine endometrial explants collected during 120 to 180 days of the gestational period. Polish Journal of Veterinary Sciences 2021, 24(1): 5-12 (10.24425/pjvs.2020.135813).

  4. Płatosz, Natalia; Bączek, Natalia; Topolska, Joanna; Szawara-Nowak, Dorota; Skipor, Janina; Milewski, Stanisław; Wiczkowski, Wiesław. Chokeberry anthocyanins and their metabolites ability to cross the blood-cerebrospinal fluid barrier. Food Chemistry 2021, 346: art. no 128730 (1-11) (10.1016/j.foodchem.2020.128730).

  5. Rękawiecki, Robert; Dobrzyń, Karolina; Kowalik, Magdalena K. Steroid receptor coregulators can modulate the action of progesterone receptor during the estrous cycle in cow endometrium. Animals 2021, 11(11): art. no 3217 (1-14) (10.3390/ani11113217).

  6. Rybska, Marta; Woźna-Wysocka, Magdalena; Wąsowska, Barbara; Skrzypski, Marek; Kubiak, Magdalena; Błaszak, Beata; Łukomska, Anna; Nowak, Tomasz; Jaśkowski, Jędrzej M. Expression of transforming growth factor beta isoforms in canine endometrium with cystic endometrial hyperplasia–pyometra complex. Animals 2021, 11(3): art. no 1844 (1-14) (10.3390/ani11061844).

  7. Skipor, Janina; Szczepkowska, Aleksandra; Kowalewska, Marta; Domżalska, Małgorzata; Herman, Andrzej P.; Krawczyńska, Agata. Photoperiod alters the choroid plexus response to LPS-induced acute inflammation in ewes. Annals of Animal Science 2021, 21(2): 497-512 (10.2478/aoas-2020-0079).

  8. Szczepkowska, Aleksandra; Harazin, András; Barna, Lilla; Deli, Mária A.; Skipor, Janina. Identification of reference genes for circadian studies on brain microvessels and choroid plexus samples isolated from rats. Biomolecules 2021, 11(8): art. no 1227 (1-19) (10.3390/biom11081227).

  9. Szczepkowska, Aleksandra; Wójcik, Maciej; Tomaszewska-Zaremba, Dorota; Antushevich, Hanna; Krawczyńska, Agata; Wiechetek, Wiktoria; Skipor, Janina; Herman, Andrzej P. Acute effect of caffeine on the synthesis of pro-inflammatory cytokines in the hypothalamus and choroid plexus during endotoxin-induced inflammation in a female sheep model. International Journal of Molecular Sciences 2021, 22(24): art. no 13237 (1-13) (10.3390/ijms222413237).

  10. Wróbel, Michał H.; Młynarczuk, Jarosław. Chloroorganic (DDT) and organophosphate (malathion) insecticides impair the motor function of the bovine cervix. Toxicology and Applied Pharmacology 2021, 427: art. no 115667 (1-9) (10.1016/j.taap.2021.115667).

2020

  1. Fraser, Leyland; Paukszto, Łukasz; Mańkowska, Anna; Brym, Paweł; Gilun, Przemysław D.; Jastrzębski, Jan P.; Pareek, Chandra S.; Kumar, Dibyendu; Pierzchała, Mariusz. Regulatory potential of long non-coding RNAs (lncRNAs) in boar spermatozoa with good and poor freezability. Life-Basel 2020, 10(11): art. no 300 (1-26) (10.3390/life10110300).

  2. Młynarczuk, Jarosław J.; Górska, M.; Wróbel, Michał H. Effects of DDT, DDE, aldrin and dieldrin on prostaglandin, oxytocin and steroid hormone release from smooth chorion explants of cattle. Animal Reproduction Science 2020, 223: art. no 106623 (1-10) (10.1016/j.anireprosci.2020.106623).

  3. Rękawiecki, Robert; Dobrzyń, Karolina; Kotwica, Jan; Kowalik, Magdalena K. Progesterone receptor coregulators as factors supporting the function of the corpus luteum in cows. Genes 2020, 11(8): art. no 923 (1-12) (10.3390/genes11080923).

  4. Sirotkin, Alexander V.; Kádasi, Attila; Balaží, Andrey; Kotwica, Jan; Alrezaki, Abdulkarem; Harrath, Abdel H. Mechanisms of the direct effects of oil-related contaminants on ovarian cells. Environmental Science and Pollution Research 2020, 27: 5314-5322 (10.1007/s11356-019-07295-0).

  5. Sirotkin, Alexander V.; Kádasi, Attila; Balaží, Andrey; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. The action of benzene, resveratrol and their combination on ovarian cell hormone release. Folia Biologica 2020, 66(2): 67-71 (pdf).

  6. Sirotkin, Alexander V.; Tarko, Adam; Kotwica, Jan; Alrezaki, Abdulkarem; Harrath, Abdel H. Interrelationships between metabolic hormones, leptin and ghrelin, and oil-related contaminants in control of oxytocin and prostaglandin F release by feline ovaries. Reproductive Biology 2020, 20(2): 254-258 (10.1016/j.repbio.2020.02.003).

  7. Szczepkowska, Aleksandra; Kowalewska, Marta; Krawczyńska, Agata; Herman, Andrzej P.; Skipor, Janina. Photoperiod affects leptin action on the choroid plexus in ewes challenged with lipopolysaccharide – study on the mRNA level. International Journal of Molecular Sciences 2020, 21(20): art. no 7647 (1-16) (10.3390/ijms21207647).

  8. Tarko, Adam; Fabová, Z.; Kotwica, Jan; Valocký, I.; Alrezaki, Abdulkarem; Alwasel, Saleh; Harrath, Abdel H.; Sirotkin, Alexander V. The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel. General and Comparative Endocrinology 2020, 295: art. no 113491 (1-6) (10.1016/j.ygcen.2020.113491).

  9. Wróbel, Michał H.; Młynarczuk, Jarosław J. The effect of polychlorinated biphenyls (PCBs) on bovine oviductal contractions and LIF synthesis during estrous cycle, in vitro studies. Research in Veterinary Science 2020, 133: 188-193 (10.1016/j.rvsc.2020.09.027).

2019

  1. Herman, Andrzej P.; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Wojtulewicz, Karolina; Pawlina, Bartosz; Antushevich, Hanna; Herman, Anna; Tomaszewska-Zaremba, Dorota. Effect of central injection of neostigmine on the bacterial endotoxin induced suppression of GnRH/LH secretion in ewes during the follicular phase of the estrous cycle. International Journal of Molecular Sciences 2019, 20(18): art. no 4598 (1-19) (10.3390/ijms20184598).

  2. Kowalik, Magdalena K.; Dobrzyń, Karolina; Rękawiecki, Robert; Kotwica, Jan. Expression of membrane progestin receptors (mPRs) α, β and γ in the bovine uterus during the oestrous cycle and pregnancy. Theriogenology 2019, 140: 171-179 (10.1016/j.theriogenology.2019.08.028).

  3. Sirotkin, Alexander V.; Benčo, Andrej; Kotwica, Jan; Alwasel, Saleh H.; Harrat, Abdel H. Apoptosis signal-regulating kinase (ASK-1) controls ovarian cell functions. Reproduction, Fertility and Development 2019, 31(11): 1657-1664 (10.1071/RD19055).

  4. Sirotkin, Alexander V.; Benčo, Andrej; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. Apoptosis signal-regulating kinase controls porcine ovarian granulosa cell functions and their response to ghrelin. Journal of Biological Regulators and Homeostatic Agents 2019, 33(5): 1479-1483 (10.23812/19-96).

  5. Sirotkin, Alexander V.; Benčo, Andrej; Mlynček, Milos; Harrath, Abdel H.; Alwasel, Saleh; Kotwica, Jan. The involvement of the phosphorylatable and nonphosphorylatable transcription factor CREB-1 in the control of human ovarian cell functions. Comptes Rendus Biologies 2019, 342(3-4): 90-96 (10.1016/j.crvi.2019.03.002).

  6. Sirotkin, Alexander V.; Benčo, Andrej; Mlynček, Milos; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. Transcription factor p53 regulates healthy human ovarian cells function. Comptes Rendus Biologies 2019, 342(5-6): 186-191 (10.1016/j.crvi.2019.08.002).

  7. Szczepkowska, Aleksandra; Kowalewska, Marta; Skipor, Janina. Melatonin from slow-release implants upregulates claudin-2 in the ovine choroid plexus. Journal of Physiology and Pharmacology 2019, 70(2): 249-254 (10.26402/jpp.2019.2.08).

  8. Tarko, Adam; Štochmal’ová, Aneta; Jedličková, Katarína; Hrabovszká, Sandra; Vachanová, Adriana; Harrath, Abdel H.; Alwasel, Saleh; Alrezaki, Abdulkarem; Kotwica, Jan; Baláži, +; Sirotkin, Alexander V. Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release. Archives Animal Breeding 2019, 62: 345-351 (10.5194/aab-62-345-2019).

2018

  1. Herman, Andrzej P.; Tomaszewska-Zaremba, Dorota; Kowalewska, Marta; Szczepkowska, Aleksandra; Oleszkiewicz, Małgorzata; Krawczyńska, Agata; Wójcik, Maciej; Antushevich, Hanna; Skipor, Janina. Neostigmine attenuates proinflammatory cytokine expression in preoptic area but not choroid plexus during lipopolysaccharide-induced systemic inflammation. Mediators of Inflammation 2018, 2018: art. no 9150207 (1-9) (10.1155/2018/9150207).

  2. Kościukiewicz, Kinga; Wylot, Bartosz; Czelejewska, Wioleta; Gilun, Przemysław D.; Okrasa, Stanisław. The effects of mu-, delta- and kappa-opioid receptor activation on in vitro prolactin secretion by anterior pituitary cells of cyclic gilts. Journal of Animal and Feed Sciences 2018, 27(2): 114-122 (10.22358/jafs/91395/2018).

  3. Kowalik, Magdalena K.; Rękawiecki, Robert; Kotwica, Jan. Expression of membrane progestin receptors (mPRs) in the bovine corpus luteum during the estrous cycle and first trimester of pregnancy. Domestic Animal Endocrinology 2018, 63: 69-76 (10.1016/j.domaniend.2017.12.004).

  4. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Mietelska, Katarzyna; Kordan, Władysław. Determination of the activity and relative abundance of mRNA for antioxidant enzymes in stallion testicular and epididymal tissues: A comparison between two breeding seasons. Animal Reproduction Science 2018, 196: 230-238 (10.1016/j.anireprosci.2018.08.015).

  5. Rękawiecki, Robert; Kisielewska, Katarzyna; Kowalik, Magdalena K.; Kotwica, Jan. Methylation of progesterone receptor isoform A and B promoters in the reproductive system of cows. Reproduction, Fertility and Development 2018, 30(12): 1634-1642 (10.1071/RD17518).

  6. Szczepkowska, Aleksandra; Skowroński, Mariusz T.; Kowalewska, Marta; Milewski, Stanisław; Skipor, Janina. Effect of melatonin from slow-release implants on aquaporins (AQP1 and AQP4) in the ovine choroid plexus. Czech Journal of Animal Science 2018, 63(1): 32-42 (10.17221/6/2017-CJAS).

  7. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. The protein expression disorders of connexins (Cx26, Cx32 and Cx43)and keratin 8 in bovine placenta under the influence of DDT, DDE and PCBs. Polish Journal of Veterinary Sciences 2018, 21(4): 721-729 (10.24425/124311).

  8. Wróbel, Michał H. Glyphosate affects the secretion of regulators of uterine contractions in cows while it does not directly impair the motoric function of myometrium in vitro. Toxicology and Applied Pharmacology 2018, 349: 55-61 (10.1016/j.taap.2018.04.031).

  9. Wróbel, Michał H. Do chlorinated insecticides (aldrin and DDT) or products of their transformations (dieldrin and DDE) impair signal transfer from regulators (oxytocin and relaxin) of bovine myometrium motility in vitro? Environmental Research 2018, 167: 234-239 (10.1016/j.envres.2018.07.027).

  10. Wróbel, Michał H.; Młynarczuk, Jarosław J. Chlorinated insecticides (toxaphene and endrin) affect oxytocin, testosterone, oestradiol and prostaglandin secretion from ovarian and uterine cells as well as myometrial contractions in cow in vitro. Chemosphere 2018, 198: 432-441 (10.1016/j.chemosphere.2018.01.143).

2017

  1. Földešiová, Martina; Baláži, Andrej; Chrastinová, Lubica; Pivko, Juraj; Kotwica, Jan; Harrath, Abdel H.; Chrenek, Peter; Sirotkin, Alexander V. Yucca schidigera can promote rabbit growth, fecundity, affect the release of hormones in vivo and in vitro, induce pathological changes in liver, and reduce ovarian resistance to benzene. Animal Reproduction Science 2017, 183: 66-76 (10.1016/j.anireprosci.2017.06.001).

  2. Herman, Andrzej P.; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Wojtulewicz, Karolina; Antushevich, Hanna; Herman, Anna; Paczesna, Kamila; Romanowicz, Katarzyna; Tomaszewska-Zaremba, Dorota. Peripheral inhibitor of AChE, neostigmine, prevents the inflammatory dependent suppression of GnRH/LH secretion during the follicular phase of the estrous cycle. BioMed Research International 2017, 2017: art. no 6823209 (1-12) (10.1155/2017/6823209).

  3. Kowalewska, Marta; Herman, Andrzej P.; Szczepkowska, Aleksandra; Skipor, Janina. The effect of melatonin from slow-release implants on basic and TLR-4-mediated gene expression of inflammatory cytokines and their receptors in the choroid plexus in ewes. Research in Veterinary Science 2017, 113: 50-55 (10.1016/j.rvsc.2017.09.003).

  4. Kowalewska, Marta; Szczepkowska, Aleksandra; Herman, Andrzej P.; Pellicer-Rubio, Maria T.; Jałyński, Marek; Skipor, Janina. Melatonin from slow-release implants did not influence the gene expression of the lipopolysaccharide receptor complex in the choroid plexus of seasonally anoestrous adult ewes subjected or not to a systemic inflammatory stimulus. Small Ruminant Research 2017, 147: 1-7 (10.1016/j.smallrumres.2016.11.018).

  5. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Koziorowski, Marek; Kordan, Władysław. Expression of Hypoxia-Inducible Factor-1α and association with Cu/Zn superoxide dismutase in the reproductive tract tissues of adult male roe deer (Capreolus capreolus) during the reproduction season. Annals of Animal Science 2017, 17(4): 1063-1074 (10.1515/aoas-2017-0008).

  6. Kwiatkowska, Joanna; Wąsowska, Barbara; Gilun, Przemysław D. Expression of hypoxia inducible factor 1α and antioxidant enzymes: Superoxide dismutases-1 and -2 in ischemic porcine endometrium. Reproductive Biology 2017, 17(3): 289-293 (10.1016/j.repbio.2017.05.013).

  7. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Steroid hormone receptors in the corpus luteum. W: The life cycle of the Corpus Luteum. Ed.: Meidan, Rina. Springer International Publishing, Switzerland (doi: 10.1007/978-3-319-43238-0) 2017 (ISBN 9783319432366), Chapter 5: 79-98 (10.1007/978-3-319-43238-0_5).

  8. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. The expression of progesterone receptor coregulators mRNA and protein in corpus luteum and endometrium of cows during the estrous cycle. Animal Reproduction Science 2017, 183: 102-109 (10.1016/j.anireprosci.2017.05.011).

  9. Skipor, Janina; Kowalewska, Marta; Szczepkowska, Aleksandra; Majewska, Anna; Misztal, Tomasz; Jałyński, Marek; Herman, Andrzej P.; Ząbek, Katarzyna. Plasma and cerebrospinal fluid interleukin-1β during lipopolysaccharide-induced systemic inflammation in ewes implanted or not with slow-release melatonin. Journal of Animal Science and Biotechnology 2017, 8: art. no 76 (1-8) (10.1186/s40104-017-0206-0).

  10. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. Disorders in barrier protein mRNA expression and placenta secretory activity under the influence of polychlorinated biphenyls in vitro. Theriogenology 2017, 89: 9-19 (10.1016/j.theriogenology.2016.10.007).

  11. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. Changes in the mRNA expression of structural proteins, hormone synthesis and secretion from bovine placentome sections after DDT and DDE treatment. Toxicology 2017, 375: 1-9 (10.1016/j.tox.2016.11.016).

  12. Wróbel, Michał H. The preliminary study about the involvement of relaxine in adverse effect of polychlorinated biphenyls on bovine myometrial contractions. Journal of Clinical and Molecular Endocrinology 2017, 2(1): art. no 5 (1-6) (10.21767/2572-5432.10039).

  13. Wróbel, Michał H.; Młynarczuk, Jarosław J. Secretory function of ovarian cells and myometrial contractions in cow are affected by chlorinated insecticides (chlordane, heptachlor, mirex) in vitro. Toxicology and Applied Pharmacology 2017, 314: 63-71 (10.1016/j.taap.2016.11.011).

  14. Wróbel, Michał H.; Młynarczuk, Jarosław J. The inhibition of myometrial contractions by chlorinated herbicides (atrazine and linuron), and their disruptive effect on the secretory functions of uterine and ovarian cells in cow, in vitro. Pesticide Biochemistry and Physiology 2017, 142: 44-52 (10.1016/j.pestbp.2017.01.002).

2016

  1. Kowalik, Magdalena K.; Martyniak, Marcin; Rękawiecki, Robert; Kotwica, Jan. Expression and immunolocalization of membrane progesterone receptors in the bovine oviduct. Domestic Animal Endocrinology 2016, 55: 83-96 (10.1016/j.domaniend.2015.12.001).

  2. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium. Reproduction, Fertility and Development 2016, 28(7): 907-913 (10.1071/RD14325).

  3. Romerowicz-Misielak, Maria; Tabęcka-Łonczyńska, Anna; Kozioł, Katarzyna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Och, Waldemar; Koziorowski, Marek. Changes in gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor gene expression after an increase in carbon monoxide concentration in the cavernous sinus of male wild boar and pig crossbreed. Journal of Physiology and Pharmacology 2016, 67(3): 431-442 (pdf).

  4. Sirotkin, Alexander V.; Mertin, Dušan; Süvegová, Karin; Harrath, Abdel H.; Kotwica, Jan. Aging influences steroid hormone release by mink ovaries and their response to leptin and IGF-I. Biology Open 2016, 5(2): 174-177 (10.1242/bio.016436).

  5. Sirotkin, Alexander V.; Mertin, Dušan; Süvegová, Karina; Lauricik, Jozef; Morovic, Martin; Harrath, Abdel H.; Kotwica, Jan. Mink aging is associated with a reduction in ovarian hormone release and the response to FSH and ghrelin. Theriogenology 2016, 86(5): 1175-1181 (10.1016/j.theriogenology.2016.04.007).

  6. Szczepkowska, Aleksandra; Lagaraine, Christine; Robert, Vincent; Młynarczuk, Jarosław J.; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. PCB153 (2,2′,4,4′,5,5′-hexachlorobiphenyl) differentially affects the VEGF/VEGFR system depending on photoperiod in the ovine choroid plexus. Ecotoxicology and Environmental Safety 2016, 124: 303-308 (10.1016/j.ecoenv.2015.11.007).

2015

  1. Bochenek, Joanna; Skipor, Janina; Kowalewska, Marta; Herman, Andrzej P. The Toll-like receptors mRNA expression profile in the pineal gland of sheep during long and short days. Journal of Animal and Feed Sciences 2015, 24(3): 208-215 (10.22358/jafs/65626/2015).

  2. Cieślak, Magdalena; Chmielewska, Natalia; Romanowicz, Katarzyna; Młynarczuk, Jarosław J.; Wańkowska, Marta. Sex-dependent differences in the effect of early weaning on the chosen hormones secretion in sheep during the postnatal transition to puberty – preliminary results. Polish Journal of Veterinary Sciences 2015, 18(1): 71-78 (10.1515/pjvs-2015-0009).

  3. Herman, Andrzej P.; Bochenek, Joanna; Skipor, Janina; Król, Karolina; Krawczyńska, Agata; Antushevich, Hanna; Pawlina, Bartosz; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota. Interleukin-1β modulates melatonin secretion in ovine pineal gland: ex vivo study. BioMed Research International 2015, 2015: art. no 526464 (1-10) (10.1155/2015/526464).

  4. Herman, Andrzej P.; Herman, Anna; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Tomaszewska-Zaremba, Dorota. Caffeine stimulates in vitro pituitary LH secretion in lipopolysaccharide-treated ewes. Reproductive Biology 2015, 15(1): 20-26 (10.1016/j.repbio.2014.12.001).

  5. Koziorowska-Gilun, Magdalena; Fraser, Leyland; Gilun, Przemysław D.; Koziorowski, Marek; Kordan, Władysław. Activity of antioxidant enzymes and their mRNA expression in different reproductive tract tissues of the male roe deer (Capreolus capreolus) during the pre-rut and rut seasons. Small Ruminant Research 2015, 129: 97-103 (10.1016/j.smallrumres.2015.05.006).

  6. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. New facts and the concept of physiological regulation of the dopaminergic system function and its disorders. Journal of Physiology and Pharmacology 2015, 66(3): 331-341 (pdf).

  7. Młynarczuk, Jarosław J.; Kotwica, Jan. Influence of 4-hydroxylated polychlorinated biphenyls on the secretory function of bovine ovarian cells: Role of the steroidogenic factor-1 receptor. Animal Reproduction Science 2015, 155: 89-98 (10.1016/j.anireprosci.2015.02.005).

  8. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Cloning and expression of progesterone receptor isoforms A and B in bovine corpus luteum. Reproduction, Fertility and Development 2015, 27(7): 1029-1037 (10.1071/RD13308).

  9. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Onapristone (ZK299) and mifepristone (RU486) regulate the messenger RNA and protein expression levels of the progesterone receptor isoforms A and B in the bovine endometrium. Theriogenology 2015, 84(3): 348-357 (10.1016/j.theriogenology.2015.03.024).

  10. Romerowicz-Misielak, Maria; Oren, Dan A.; Sowa-Kućma, Magdalena; Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Koziorowski, Marek. Changes in melatonin synthesis parameters after carbon monoxide concentration increase in the cavernous sinus. Journal of Physiology and Pharmacology 2015, 66(4): 505-514 (pdf).

  11. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. Short-term incubation of bovine placentome sections as a tool to study xenobiotic mechanism of action. Reproductive Biology 2015, 15(4): 238-246 (10.1016/j.repbio.2015.10.002).

  12. Skipor, Janina; Szczepkowska, Aleksandra; Kowalewska, Marta; Herman, Andrzej P.; Lisiewski, Paweł. Profile of toll-like receptor mRNA expression in the choroid plexus in adult ewes. Acta Veterinaria Hungarica 2015, 63(1): 69-78 (10.1556/AVet.2014.027).

  13. Teixeira-Gomes, Ana-Paul; Harichaux, Gregoire; Gennetay, Dominique; Skipor, Janina; Thiéry, Jean-Claude; Labas, Valerie; Dufourny, Laurence. Photoperiod affects the cerebrospinal fluid proteome: a comparison between short day- and long day-treated ewes. Domestic Animal Endocrinology 2015, 53: 1-8 (10.1016/j.domaniend.2015.04.003).

  14. Wiczkowski, Wiesław; Skipor, Janina; Misztal, Tomasz; Szawara-Nowak, Dorota; Topolska, Joanna; Piskuła, Mariusz K. . Quercetin and isorhamnetin aglycones are the main metabolites of dietary quercetin in cerebrospinal fluid. Molecular Nutrition & Food Research 2015, 59(6): 1088-1094 (10.1002/mnfr.201400567).

  15. Woźny, Maciej; Jakimiuk, Ewa; Brzuzan, Paweł; Florczyk, Maciej; Niewiadowska, Alicja; Obremski, Kazimierz; Gajęcka, Magdalena; Młynarczuk, Jarosław J.; Gajęcki, Maciej. Dichlorodiphenyldichloroethylene (DDE) residue limit exceeded in pig tissues after feed-borne exposure to maximum allowed concentration. Environmental Biotechnology 2015, 11(1): 1-9 (10.14799/ebms242).

  16. Wróbel, Michał H.; Grzeszczyk, Marlena; Młynarczuk, Jarosław J.; Kotwica, Jan. The adverse effects of aldrin and dieldrin on both myometrial contractions and the secretory functions of bovine ovaries and uterus in vitro. Toxicology and Applied Pharmacology 2015, 285(1): 23-31 (10.1016/j.taap.2015.03.005).

2014

  1. Cejko, Beata I.; Młynarczuk, Jarosław; Kowalski, Radosław K. Pozyskiwania nasienia karpia w warunkach kontrolowanych bez stosowania stymulacji hormonalnej. W: Szkolenie producentów ryb. Materiały szkoleniowe. Ed.: Ferlin, M. Polskie Towarzystwo Rybackie, Poznań 2014 (ISBN 9788393904518), 144-150.

  2. Jabłońska, Olga; Piasecka-Srader, Joanna; Nynca, Anna; Kołomycka, Agnieszka; Robak, Anna; Wąsowska, Barbara; Ciereszko, Renata E. 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters steroid secretion but does not affect cell viability and the incidence of apoptosis in porcine luteinized granulosa cells. Acta Veterinaria Hungarica 2014, 62(3): 408-421 (10.1556/AVet.2014.015).

  3. Kowalik, Magdalena K.; Rękawiecki, Robert; Kotwica, Jan. Expression and localization of progesterone receptor membrane component 1 and 2 and serpine mRNA binding protein 1 in the bovine corpus luteum during the estrous cycle and the first trimester of pregnancy. Theriogenology 2014, 82(8): 1086-1093 (10.1016/j.theriogenology.2014.07.021).

  4. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława; MusZak, Jolanta; Gilun, Przemysław D.; Koziorowski, Marek. Cavernous sinus and its mysterious physiological functions: facts and hypotheses. Acta Biologica Cracoviensia Series Zoologia 2014, 55-56: 7-15 (pdf).

  5. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Kotwica, Jan. The orphan nuclear receptor SF-1 is involved in the effect of PCBs, DDT, and DDE on the secretion of steroid hormones and oxytocin from bovine luteal cells during the estrous cycle in vitro. Theriogenology 2014, 81(7): 877-886 (10.1016/j.theriogenology.2014.01.016).

  6. Muszak, Jolanta; Krzymowski, Tadeusz; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława. Countercurrent transfer of dopamine from venous blood in the cavernous sinus to the arterial blood supplying the brain – the perfused rabbit head as an experimental model. Journal of Physiology and Pharmacology 2014, 65(5): 641-648 (pdf).

  7. Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Koziorowski, Marek. Local transfer of testosterone and aromatase activity in the spermatic cord in wild boar/pig hybrids in short-daylight and long-daylight periods. Reproductive Biology 2014, 14(4): 282-288 (10.1016/j.repbio.2014.06.004).

  8. Wróbel, Michał H.; Będziechowski, Paweł; Młynarczuk, Jarosław J.; Kotwica, Jan. Impairment of uterine smooth muscle contractions and prostaglandin secretion from cattle myometrium and corpus luteum in vitro is influenced by DDT, DDE and HCH. Environmental Research 2014, 132: 54-56 (10.1016/j.envres.2014.02.018).

2013

  1. Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Romerowicz-Misielak, Maria; Tabęcka-Łonczyńska, Anna; Przekop, Franciszek; Koziorowski, Marek. Carbon monoxide-mediated humoral pathway for the transmission of light signal to the hypothalamus. Journal of Physiology and Pharmacology 2013, 64(6): 761-772 (pdf).

  2. Kowalik, Magdalena K.; Rękawiecki, Robert; Kotwica, Jan. The putative roles of nuclear and membrane-bound progesterone receptors in the female reproductive tract. Reproductive Biology 2013, 13(4): 279-289 (10.1016/j.repbio.2013.09.001).

  3. Kowalik, Magdalena K.; Słonina, Dominika; Rękawiecki, Robert; Kotwica, Jan. Expression of progesterone receptor membrane component (PGRMC) 1 and 2, serpine mRNA binding protein 1 (SERBP1) and nuclear progesterone receptor (PGR) in the bovine endometrium during the estrous cycle and the first trimester of pregnancy. Reproductive Biology 2013, 13(1): 15-23 (10.1016/j.repbio.2013.01.170).

  4. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Fraser, Leyland; Koziorowski, Marek; Stefańczyk-Krzymowska, Stanisława; Kordan, Władysław. Antioxidant enzyme activity and mRNA expression in reproductive tract of adult male european bison (Bison bonasus, Linnaeus 1758). Reproduction in Domestic Animals 2013, 48(1): 7-14 (10.1111/j.1439-0531.2012.02015.x).

  5. Młynarczuk, Jarosław J.; Kowalik, Magdalena K. The effect of PCB126, 77, and 153 on the intracellular mobilization of Ca+2 in bovine granulosa and luteal cells after FSH and LH surge in vitro. Polish Journal of Veterinary Sciences 2013, 16(3): 417-424 (10.2478/pjvs-2013-0059).

  6. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Kotwica, Jan. Adverse influence of coumestrol on synthesis and secretion of oxytocin and progesterone from bovine luteal cells in the first trimester of pregnancy. Environmental Toxicology 2013, 28(7): 411-418 (10.1002/tox.20735).

  7. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Rękawiecki, Robert; Kotwica, Jan. The expression of Steroidogenic Factor-1 and its role in bovine steroidogenic ovarian cells during the estrus cycle and first trimester of pregnancy. Animal Reproduction Science 2013, 138(1-2): 74-81 (10.1016/j.anireprosci.2013.01.008).

  8. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Ziółkowska, Anna; Kotwica, Jan. Involvement of the orphan nuclear receptor SF-1 in the effect of PCBs, DDT and DDE on the secretion of steroid hormones and oxytocin from bovine granulosa cells. Animal Reproduction Science 2013, 143(1-4): 30-37 (10.1016/j.anireprosci.2013.10.007).

  9. Muszak, Jolanta. The impact of selected vasoactive factors on vascular functions. Polish Annals of Medicine – Rocznik Medyczny 2013, 20(2): 149-153 (10.1016/j.poamed.2013.09.001).

  10. Nynca, Anna; Nynca, Joanna; Wąsowska, Barbara; Kolesarova, Adriana; Kołomycka, Agnieszka; Ciereszko, Renata E. Effects of the phytoestrogen, genistein, and protein tyrosine kinase inhibitor-dependent mechanisms on steroidogenesis and estrogen receptor expression in porcine granulosa cells of medium follicles. Domestic Animal Endocrinology 2013, 44(1): 10-18 (10.1016/j.domaniend.2012.07.002).

  11. Nynca, Anna; Słonina, Dominika; Jabłońska, Olga; Kamińska, Barbara; Ciereszko, Renata E. Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs. Acta Veterinaria Hungarica 2013, 61(1): 85-98 (10.1556/AVet.2012.060).

  12. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Validation of housekeeping genes for studying differential gene expression in the bovine myometrium. Acta Veterinaria Hungarica 2013, 61(4): 505-516 (10.1556/AVet.2013.037).

  13. Szczepkowska, Aleksandra; Lagaraine, Christine; Robert, Vincent; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. Effect of a two-week treatment with low dose of 2,2’,4,4’,5,5’ hexachlorobiphenyl (PCB153) on tight junction proteins expression in ovine choroid plexus during long and short photoperiod. Neurotoxicology and Teratology 2013, 37: 63-67 (10.1016/j.ntt.2013.03.061).

  14. Ziółkowska, Anna; Młynarczuk, Jarosław J.; Kotwica, Jan. Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-α-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells. Polish Journal of Veterinary Sciences 2013, 16(2): 231-239 (10.2478/pjvs-2013-0033).

2012

  1. Grzegorzewski, Waldemar J.; MusZak, Jolanta; Wąsowska, Barbara; Jana, Barbara; Stefańczyk-Krzymowska, Stanisława. The influence of steroids on noradrenaline-mediated contractile reactivity of the superficial nasal and facia veins in cycling gilts. Polish Journal of Veterinary Sciences 2012, 15(2): 297-304 (10.2478/v10181-011-0147-z).

  2. Koziorowski, Marek; Stefańczyk-Krzymowska, Stanisława; Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Kamiński, Marian. The gaseous messenger carbon monoxide is released from the eye into the ophthalmic venous blood depending on the intensity of sunlight. Journal of Biological Regulators and Homeostatic Agents 2012, 26(1): 111-118.

  3. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. Local retrograde and destination transfer of physiological regulators as an important regulatory system and its role. Facts and hypothesis. Journal of Physiology and Pharmacology 2012, 63(1): 3-16 (pdf).

  4. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. Advances in understanding the physiological mechanism of maternal immune tolerance to the embryo. Reproductive Biology 2012, 12(3): 265-270 (10.1016/j.repbio.2012.10.004).

  5. Rękawiecki, Robert; Rutkowska, Joanna; Kotwica, Jan. Identification of optimal housekeeping genes for examination of gene expression in bovine corpus luteum. Reproductive Biology 2012, 12(4): 362-367 (10.1016/j.repbio.2012.10.010).

  6. Sirotkin, Alexander V.; Kadasi, Attila; Balazi, Andrey; Bakova, Zuzana; Harrath, Abdel H.; Makarevich, Alexander V.; Kolesarova, Adriana; Chrenek, Peter; Kotwica, Jan; Toth, Tomas. Influence of petrochemical industry environmental contaminants on animal ovarian cells. Journal of Microbiology; Biotechnology and Food Sciences 2012, 2(2): 517-525 (pdf).

  7. Skipor, Janina; Misztal, Tomasz; Piskuła, Mariusz K.; Wiczkowski, Wiesław; Thiéry, Jean-Claude. Phytoestrogens and thyroid hormone levels in the cerebrospinal fluid of ewes fed red clover silage. Small Ruminant Research 2012, 102(2-3): 157-162 (10.1016/j.smallrumres.2011.07.012).

  8. Skipor, Janina; Młynarczuk, Jarosław J.; Szczepkowska, Aleksandra; Lagaraine, Christine; Grochowalski, Adam; Guillame, Daniel; Dufourny, Laurence; Thiéry, Jean-Claude. Photoperiod modulates access of 2,2’,4,4’,5,5’-hexachlorobiphenyl (PCB153) to the brain and its effect on gonadotropin and thyroid hormones in adult ewes. Ecotoxicology and Environmental Safety 2012, 78: 336-343 (10.1016/j.ecoenv.2011.11.042).

  9. Słonina, Dominika; Kowalik, Magdalena K.; Kotwica, Jan. Expression of progesterone receptor membrane component 1, serpine mRNA binding protein 1 and nuclear progesterone receptor isoforms A and B in the bovine myometrium during estrous cycle and early pregnancy. Journal of Reproduction and Development 2012, 58(3): 288-294 (10.1262/jrd.11-052T).

  10. Stefańczyk-Krzymowska, Stanisława; Wąsowska, Barbara; Gilun, Przemysław D.; MusZak, Jolanta; Grzegorzewski, Waldemar J. Relationship between contractions of the uterus and concentration of PGF2α in uterine venous blood after luteolysis in gilts. Reproduction in Domestic Animals 2012, 47(1): 98-104 (10.1111/j.1439-0531.2011.01807.x).

  11. Szczepkowska, Aleksandra; Młynarczuk, Jarosław J.; Grochowalski, Adam; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. Effect of a two-week treatment with low dose of ortho-substituted polychlorinated biphenyls (PCB104) and PCR153) on VRGF-receptor system expression in the choroid plexus in adult ewes. Polish Journal of Veterinary Sciences 2012, 15(4): 621-628 (10.2478/v10181-012-0098-z).

  12. Szczepkowska, Aleksandra; Wąsowska, Barbara; Gilun, Przemysław D.; Lagaraine, Christine; Robert, Vincent; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. Pattern of expression of vascular endothelial growth factor and its receptors in ovine choroid plexus during long and short photoperiod. Cell and Tissue Research 2012, 350(1): 157-166 (10.1007/s00441-012-1431-7).

  13. Wróbel, Michał H.; Młynarczuk, Jarosław J.; Kotwica, Jan. The effect of DDT and its metabolite (DDE) on prostaglandin secretion from epithelial cells and on contractions of the smooth muscle of the bovine oviduct in vitro. Toxicology and Applied Pharmacology 2012, 259(2): 152-159 (10.1016/j.taap.2011.12.019).

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Foreign co-operation P&ToR

  1. Research Institute of Animal Production, Nitra, Slovak Republic, prof. Alexander V. Sirotkin
  2. Department of Animal Science, University of Kentucky, Lexington, USA, prof. William J. Silvia
  3. Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Rumunia, prof. Horia Cernescu

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Achievements P&ToR

In our studies, it has been demonstrated:

  • The importance of adrenergic stimulation in the corpus luteum function;
  • The mechanism of autoregulation of corpus luteum function ;
  • Non-genomic effect of steroids on the uterus and ovary function;
  • The disturbing effect of xenobiotics on the function of reproductive tract, through the genomic and non-genomic way.

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Current projects P&ToR

  1. J.Kotwica – Effect of organochlorine xenobiotics on state of maternal-foetal connections in pregnant cows.
  2. M.Kowalik – The role of steroid hormones and lutetropic and luteolytic factors in regulation of the expression and function of membrane progesterone receptors in bovine reproductive tract.

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Equipment and applied methods LPR

Applied methods

1. In vivo methods

  • measurement of blood flow in the uterine artery of the pig by an electric transducer using an electromagnetic flowmeter (RT500 Narcomatic) under  general anaesthesia
  • measurement of porcine uterine intraluminal pressure/indirect uterine contractile activity (Bridge Amp AD Instruments) under  general anaesthesia
  • biotechnology methods of reproduction in the sheep: synchronization of ovulation (intravaginal suppositories) and acceleration of the breeding season (melatonin implants) for experimental purposes
  • mid-ventral laparotomy of the pig and sheep: ovariectomy, hysterectomy, induction of ischemia in the uterus of the pig by ligation of the uterine artery, intravital collection of uterine samples, implantation of catheters to blood vessels of mesometrium for blood collection and/or infusion of biologically active substances e.g. hormones under  general anaesthesia
  • implantation of catheters into blood vessels of the head and neck in the pig, sheep and rabbit under  general anaesthesia
  • long-term collection of cerebrospinal fluid from the third brain ventricle in sheep
  • long-term blood collection from jugular veins of the pig and sheep
  • continuous monitoring of blood oxygen saturation, pulse rate data, carbon dioxide detection and visual respiration indicator using pulse oximeter with carbon dioxide detector (Nonin Medical Inc. 9847V) under general anaesthesia

2. Ex vivo methods

  • the study of the contractile activity of the superficial veins of the head in the pig (frontal, nasal and facial veins)
  • the study of the contractile activity of isolated arteries of the female reproductive tract in the pig (the uterine artery)
  • the study on the isolated head of the pig, sheep, rabbit perfused with autologous blood
  • the study on the isolated pig’s ovary and uterus, testicle of the pig and rat perfused with autologous blood

3. Tissue incubations

  • pig’s hypothalamus explants

4. Analytic methods

4.1  Molecular biology methods

Expression on the mRNA level: Real Time PCR (qPCR) or Real Time PCR with TaqMan probes (qPCR TaqMan) and on the protein level: Western blot analysis (WB):

  • factors associated with production of cerebrospinal fluid: VEGF and its receptors: Flt-1, Flk-1 and co-receptor NRP-1, aquaporin-1,  aquaporin-4, claudin-2 (qPCR, WB) in the ovine choroid plexus
  • tight junction proteins restricting the passage of molecules via the intercellular path: transmembrane proteins: occludin, claudin-1, JAM-1 and cytosolic (scaffolding) proteins: ZO-1, ZO-2, ZO-3 and afadin-6 (qPCR, WB) in the ovine choroid plexus
  • transport proteins: transtyretin (TTR) in the ovine choroid plexus (qPCR)
  • lipopolysaccharide receptor complex: toll-like receptor 4 (TLR4), CD14 and MD-2 in the ovine choroid plexus (qPCR)
  • cytokines and their receptors and chemokines: TNFα, IL-1β, IL-6, TNFRI, TNFRII, IL-1R1, IL-1R2, IL-6R, gp130, MCP-1 in ovine choroid plexus (qPCR)
  • metalloproteinases and their tissue inhibitors: MMP2, MMP3, MMP9, TIMP-1 and TIMP-3 (qPCR) in the ovine choroid plexus
  • hypoxia marker HIF-1α in in the porcine uterus (qPCR, WB)
  • biological clock elements: Bmal 1, Clock, Npas2, Per 1-3, Cry 1-2 (qPCR TaqMan, WB) in the hypothalamus of domestic pig
  • nuclear receptors associated to the functioning of molecular biological clock: Rev-erb α, Rev-erb β, Ror α, Ror β, Ror γ (qPCR TaqMan) in the hypothalamus of domestic pig
  • antioxidant enzymes in the testis and epididymis of male deer and bison: EcSOD, GpX 4, GpX 5 (qPCR), SOD-1, Cat (qPCR, WB) and SOD-1, SOD-2 in porcine uterus (qPCR)
  • leptin receptor (Ob-Ra i Ob-Rb) in the choroid plexus and hypothalamus of domestic pig (qPCR, WB)

4.2 Immunoenzymatic (ELISA) method:

  • LPS Binding Protein in sheep
  • IL-6 in sheep

4.3 Radioimmunoassay (RIA) in plasma, tissues and body fluids:

  • thyroid hormones: T3 and T4 (free and total)
  • melatonin
  • hormones of reproductive system: estradiol, estrone, progesterone, testosterone, oxytocin, prostaglandin F2α
  • protein hormones: prolactin (PRL), luteinizing hormone (LH), follicle stimulating hormone (FSH), leptin, activin A in pig

4.4 Labeling hormones with radioisotope

  • oxytocin, GnRH, FSH, LH, prolactin, β-endorphin iodination (J125)

4.5 Immunohistochemical localization (with fluorochrome):

  • c-Fos protein (early response gene product) in porcine brain
  • NADPH-diaphorase (histochemical method) in the blood vessels of porcine brain
  • nitric oxide synthases, iNOS, eNOS in the porcine uterus and periovarian vascular complex and in rat’s large intestine
  • endogenous hypoxia marker (HIF-1α) in porcine oviduct and uterus
  • antioxidant enzymes SOD-1 and SOD-2 in porcine female reproductive tract and mice’s large intestine
  • Vascular Endothelial Growth Factor (VEGF) and its receptors Flt-1, Flk-1 in the ovine choroid plexus
  • antiapoptotic protein cFLIP in porcine female reproductive tract
  • apoptotic cells with TUNEL assay (Terminal Transferase dUTP Nick End Labeling) in porcine uterus and rat’s large intestine
  • dopamine hydroxylase (DβH) in porcine head vessels (facial, nasal and frontal vein) and peri-ovarian vascular complex
  • leptin receptor in porcine hypothalamus and choroid plexus
  • lipopolysaccharide receptors complex: CD14 and TLR4 in choroid plexus in sheep

4.6 Optical density measurments:

  • immunoreactivity measurement with Axio Vision Rel. 4.8.2 program

Equipment

1. Equipment for in vivo, ex vivo experiments:

  • Inhaled anesthetic apparatus for large animals (KOMESAROFF MINI-KOM)
  • Cardiomonitor (TEMED)
  • Pulse oximeter with carbon dioxide detector (Nonin Medica Inc.)
  • Set for experiments on isolated organs (UNIPER, Up-100, Hugo Sachs Elektronik-Harvard)
  • Set for measurement of isometric tension isolated blood vessels or muscle tissues (HSE F30, type 372, Hugo Sachs Elektronik-Harvard)
  • Infusion pumps
  • Peristaltic pump (Elphan, ZALIMP)
  • Electromagnetic transducers for blood flow measurement (Flowmeter Narcomatic RT500)
  • Surgical drill (Esculap)

2. Western blot and nucleic acids analysis workplace:

  1. Mini PROTEAN system with PowerPac basic power supply (electrophoresis system and wet transfer system, Bio Rad)
  2. Hematological roller (Chemland)
  3. Horizontal electrophoresis system Wide Mini Sub Cell (Bio Rad)
  4. Laminar flow chamber (Polon)

3. Equipment for Radioimmunoassay:

  • Gamma Counter (LKB)
  • Liquid Scintillation Counter (Beckman)
  • Radiometer with probe Rust-3

4. Basic lab equipment:

  • Vacuum pump BioVac 225 (OmegaLab)
  • Spectrophotometer (Beckman)
  • Stereoscopic microscope
  • Vortex (Biosan)
  • Shaker (Biosan)
  • Incubation chamber with shaker (Edmund Buehler)
  • Magnetic stirrer (Biosan, IKA WERKE)
  • Heated dry block  (OmegaLab)
  • Water bath
  • pH-meter (HANNA Instruments)
  • Mini centrifuge (Biosan, StarLab)
  • Centrifuge with cooling (MPW, Beckman)
  • Electronic scales (RADWAG, MEDICAT)
  • Ultra low freezer (SANYO)
  • Fume cupboard (Polon)
  • Ice maker (Scotsman)
  • Manifold Machine (pipettor) Hamilton Microlab 1000

Read more

Publications LPR

2024

  1. Herman, Andrzej P.; Tomczyk, Monika; Wójcik, Maciej; Bochenek, Joanna; Antushevich, Hanna; Herman, Anna; Wiechetek, Wiktoria; Szczepkowska, Aleksandra; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota. Effect of Caffeine on the Inflammatory-Dependent Changes in the GnRH/LH Secretion in a Female Sheep Model. International Journal of Molecular Sciences 2024, 25(5): art. no 2663 (1-15) (10.3390/ijms25052663).
  2. Neuman, Nicoletta M.; Dziekońska, Anna; Orzołek, Aleksandra; Gilun, Przemysław D. A comparison of the biological properties of European red deer (Cervus elaphus elaphus) spermatozoa stored in the epididymides and in a liquid state at 5 °C. Animal Reproduction Science 2024, 260: art. no 107387 (1-13) (10.1016/j.anireprosci.2023.107387).

2023

  1. Gilun, Przemysław; Koziorowska-Gilun, Magdalena; Wąsowska, Barbara; Sowa-Kućma, Magdalena; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Kordan, Władysław; Koziorowski, Marek. Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season. Polish Journal of Veterinary Sciences 2023, 26(1): 65-82 (10.24425/pjvs.2023.145008).
  2. Rybska, Marta; Billert, Maria; Skrzypski, Marek; Wojciechowicz, Tatiana; Kubiak, Magdalena; Łukomska, Anna; Nowak, Tomasz; Włodarek, Jan; Wąsowska, Barbara. Expression and localization of the neuropeptide phoenixin-14 and its receptor GRP173 in the canine reproductive organs and periovarian adipose tissue. Animal Reproduction Science 2023, 255: art. no 107282 (1-18) (10.1016/j.anireprosci.2023.107282).
  3. Szczepkowska, Aleksandra; Bochenek, Joanna; Wójcik, Maciej; Tomaszewska-Zaremba, Dorota; Antushevich, Hanna; Tomczyk, M.; Skipor, Janina; Herman, Andrzej P. Effect of caffeine on adenosine and ryanodine receptor gene expression in the hypothalamus, pituitary, and choroid plexus in ewes under basal and LPS challenge conditions. Journal of Animal and Feed Sciences 2023, 32(1): 17-25 (10.22358/jafs/156762/2022).

2022

  1. Mańkowska, Anna; Gilun, Przemysław D.; Zasiadczyk, Łukasz; Sobiech, Przemysław; Fraser, Leyland. Expression of TXNRD1, HSPA4L and ATP1B1 genes associated with the freezability of boar sperm. International Journal of Molecular Sciences 2022, 23(16): art. no 6320 (1-18) (10.3390/ijms23169320).
  2. Nowak, Sławomir; Gilun, Przemysław; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Koziorowska-Gilun, Magdalena; Wąsowska, Barbara. Carbon monoxide (CO) as a retinal regulator of heme oxygenases -1, and -2 (HO’s) expression. Biomedicines 2022, 10(2): art. no 358 (1-9) (10.3390/biomedicines10020358).
  3. Rybska, Marta; Billert, Maria; Skrzypski, Marek; Kubiak, Magdalena; Woźna-Wysocka, Magdalena; Łukomska, Anna; Nowak, Tomasz; Błaszczyk-Cichoszewska, Joanna; Pomorska-Móla, Małgorzata; Wąsowska, Barbara. Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression. Animal Reproduction Science 2022, 238: art. no 106931 (1-13) (10.1016/j.anireprosci.2022.106931).

2021

  1. Domżalska, Małgorzata; Wiczkowski, Wiesław; Szczepkowska, Aleksandra; Chojnowska, Sylwia; Misztal, Tomasz; Walter, Fruzsina R.; Deli, Maria A.; Ishikawa, Hiroshi; Schroten, Horst; Schwerk, Christian; Skipor, Janina. Effect of lipopolysaccharide-induced inflammatory challenge on β-glucuronidase activity and quercetin and its metabolites concentration in the choroid plexus, blood plasma and cerebrospinal fluid. International Journal of Molecular Sciences 2021, 22(13): art. no 7122 (1-17) (10.3390/ijms22137122).
  2. Gilun, Przemysław D.; Flisikowski, Krzysztof; Flisikowska, Tatiana; Kwiatkowska, Joanna; Wąsowska, Barbara; Koziorowska-Gilun, Magdalena. Role of methylation in period2 (PER2) transcription in the context of the presence or absence of light signals: natural and chemical – studies on the pig model. International Journal of Molecular Sciences 2021, 22(15): art. no 7796 (1-17) (10.3390/ijms22157796).
  3. Płatosz, Natalia; Bączek, Natalia; Topolska, Joanna; Szawara-Nowak, Dorota; Skipor, Janina; Milewski, Stanisław; Wiczkowski, Wiesław. Chokeberry anthocyanins and their metabolites ability to cross the blood-cerebrospinal fluid barrier. Food Chemistry 2021, 346: art. no 128730 (1-11) (10.1016/j.foodchem.2020.128730).
  4. Rybska, Marta; Woźna-Wysocka, Magdalena; Wąsowska, Barbara; Skrzypski, Marek; Kubiak, Magdalena; Błaszak, Beata; Łukomska, Anna; Nowak, Tomasz; Jaśkowski, Jędrzej M. Expression of transforming growth factor beta isoforms in canine endometrium with cystic endometrial hyperplasia–pyometra complex. Animals 2021, 11(3): art. no 1844 (1-14) (10.3390/ani11061844).
  5. Skipor, Janina; Szczepkowska, Aleksandra; Kowalewska, Marta; Domżalska, Małgorzata; Herman, Andrzej P.; Krawczyńska, Agata. Photoperiod alters the choroid plexus response to LPS-induced acute inflammation in ewes. Annals of Animal Science 2021, 21(2): 497-512 (10.2478/aoas-2020-0079).
  6. Szczepkowska, Aleksandra; Harazin, András; Barna, Lilla; Deli, Mária A.; Skipor, Janina. Identification of reference genes for circadian studies on brain microvessels and choroid plexus samples isolated from rats. Biomolecules 2021, 11(8): art. no 1227 (1-19) (10.3390/biom11081227).
  7. Szczepkowska, Aleksandra; Wójcik, Maciej; Tomaszewska-Zaremba, Dorota; Antushevich, Hanna; Krawczyńska, Agata; Wiechetek, Wiktoria; Skipor, Janina; Herman, Andrzej P. Acute effect of caffeine on the synthesis of pro-inflammatory cytokines in the hypothalamus and choroid plexus during endotoxin-induced inflammation in a female sheep model. International Journal of Molecular Sciences 2021, 22(24): art. no 13237 (1-13) (10.3390/ijms222413237).

2020

  1. Fraser, Leyland; Paukszto, Łukasz; Mańkowska, Anna; Brym, Paweł; Gilun, Przemysław; Jastrzębski, Jan P.; Pareek, Chandra S.; Kumar, Dibyendu; Pierzchała, Mariusz. Regulatory potential of long non-coding RNAs (lncRNAs) in boar spermatozoa with good and poor freezability. Life-Basel 2020, 10(11): art. no 300 (1-26) (10.3390/life10110300).
  2. Szczepkowska, Aleksandra; Kowalewska, Marta; Krawczyńska, Agata; Herman, Andrzej P.; Skipor, Janina. Photoperiod affects leptin action on the choroid plexus in ewes challenged with lipopolysaccharide – study on the mRNA level. International Journal of Molecular Sciences 2020, 21(20): art. no 7647 (1-16) (10.3390/ijms21207647).

2019

  1. Herman, Andrzej P.; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Wojtulewicz, Karolina; Pawlina, Bartosz; Antushevich, Hanna; Herman, Anna; Tomaszewska-Zaremba, Dorota. Effect of central injection of neostigmine on the bacterial endotoxin induced suppression of GnRH/LH secretion in ewes during the follicular phase of the estrous cycle. International Journal of Molecular Sciences 2019, 20(18): art. no 4598 (1-19) (doi: 10.3390/ijms20184598).
  2. Szczepkowska, Aleksandra; Kowalewska, Marta; Skipor, Janina. Melatonin from slow-release implants upregulates claudin-2 in the ovine choroid plexus. Journal of Physiology and Pharmacology 2019, 70(2): 249-254 (doi: 10.26402/jpp.2019.2.08).

2018

  1. Herman, Andrzej P.; Tomaszewska-Zaremba, Dorota; Kowalewska, Marta; Szczepkowska, Aleksandra; Oleszkiewicz, Małgorzata; Krawczyńska, Agata; Wójcik, Maciej; Antushevich, Hanna; Skipor, Janina. Neostigmine attenuates proinflammatory cytokine expression in preoptic area but not choroid plexus during lipopolysaccharide-induced systemic inflammation. Mediators of Inflammation 2018, 2018: art. no 9150207 (1-9) (doi: 10.1155/2018/9150207).
  2. Kościukiewicz, Kinga; Wylot, Bartosz; Czelejewska, Wioleta; Gilun, Przemysław D.; Okrasa, Stanisław. The effects of mu-, delta- and kappa-opioid receptor activation on in vitro prolactin secretion by anterior pituitary cells of cyclic gilts. Journal of Animal and Feed Sciences 2018, 27(2): 114-122 (doi: 10.22358/jafs/91395/2018).
  3. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Mietelska, Katarzyna; Kordan, Władysław. Determination of the activity and relative abundance of mRNA for antioxidant enzymes in stallion testicular and epididymal tissues: A comparison between two breeding seasons. Animal Reproduction Science 2018, 196: 230-238 (doi: 10.1016/j.anireprosci.2018.08.015).
  4. Szczepkowska, Aleksandra; Skowroński, Mariusz T.; Kowalewska, Marta; Milewski, Stanisław; Skipor, Janina. Effect of melatonin from slow-release implants on aquaporins (AQP1 and AQP4) in the ovine choroid plexus. Czech Journal of Animal Science 2018, 63(1): 32-42 (doi: 10.17221/6/2017-CJAS).

2017

  1. Herman, Andrzej P.; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Wojtulewicz, Karolina; Antushevich, Hanna; Herman, Anna; Paczesna, Kamila; Romanowicz, Katarzyna; Tomaszewska-Zaremba, Dorota. Peripheral inhibitor of AChE, neostigmine, prevents the inflammatory dependent suppression of GnRH/LH secretion during the follicular phase of the estrous cycle. BioMed Research International 2017, 2017: art. no 6823209 (1-12) (doi: 10.1155/2017/6823209).
  2. Kowalewska, Marta; Herman, Andrzej P.; Szczepkowska, Aleksandra; Skipor, Janina. The effect of melatonin from slow-release implants on basic and TLR-4-mediated gene expression of inflammatory cytokines and their receptors in the choroid plexus in ewes. Research in Veterinary Science 2017, 113: 50-55 (doi: 10.1016/j.rvsc.2017.09.003).
  3. Kowalewska, Marta; Szczepkowska, Aleksandra; Herman, Andrzej P.; Pellicer-Rubio, Maria T.; Jałyński, Marek; Skipor, Janina. Melatonin from slow-release implants did not influence the gene expression of the lipopolysaccharide receptor complex in the choroid plexus of seasonally anoestrous adult ewes subjected or not to a systemic inflammatory stimulus. Small Ruminant Research 2017, 147: 1-7 (doi: 10.1016/j.smallrumres.2016.11.018).
  4. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Koziorowski, Marek; Kordan, Władysław. Expression of Hypoxia-Inducible Factor-1α and association with Cu/Zn superoxide dismutase in the reproductive tract tissues of adult male roe deer (Capreolus capreolus) during the reproduction season. Annals of Animal Science 2017, 17(4): 1063-1074 (doi: 10.1515/aoas-2017-0008).
  5. Kwiatkowska, Joanna; Wąsowska, Barbara; Gilun, Przemysław D. Expression of hypoxia inducible factor 1α and antioxidant enzymes: Superoxide dismutases-1 and -2 in ischemic porcine endometrium. Reproductive Biology 2017, 17(3): 289-293 (doi: 10.1016/j.repbio.2017.05.013).
  6. Skipor, Janina; Kowalewska, Marta; Szczepkowska, Aleksandra; Majewska, Anna; Misztal, Tomasz; Jałyński, Marek; Herman, Andrzej P.; Ząbek, Katarzyna. Plasma and cerebrospinal fluid interleukin-1β during lipopolysaccharide-induced systemic inflammation in ewes implanted or not with slow-release melatonin. Journal of Animal Science and Biotechnology 2017, 8: art. no 76 (1-8) (doi: 10.1186/s40104-017-0206-0).

2016

  1. Romerowicz-Misielak, Maria; Tabęcka-Łonczyńska, Anna; Kozioł, Katarzyna; Gilun, Przemysław; Stefańczyk-Krzymowska, Stanisława; Och, Waldemar; Koziorowski, Marek. Changes in gonadotropin-releasing hormone and gonadotropinreleasing hormone receptor gene expression after an increase in carbon monoxide concentration in the cavernous sinus of male wild boar and pig crossbreed. Journal of Physiology and Pharmacology 2016, 67(3): 431-442 (pdf).
  2. Szczepkowska, Aleksandra; Lagaraine, Christine; Robert, Vincent; Młynarczuk, Jarosław; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. PCB153 (2,2′,4,4′,5,5′-hexachlorobiphenyl) differentially affects the VEGF/VEGFR system depending on photoperiod in the ovine choroid plexus. Ecotoxicology and Environmental Safety 2016, 124: 303-308 (doi: 10.1016/j.ecoenv.2015.11.007).

2015

  1. Bochenek, Joanna; Skipor, Janina; Kowalewska, Marta; Herman, Andrzej P. The Toll-like receptors mRNA expression profile in the pineal gland of sheep during long and short days. Journal of Animal and Feed Sciences 2015, 24(3): 208-215 (doi: 10.22358/jafs/65626/2015).
  2. Herman, Andrzej P.; Bochenek, Joanna; Skipor, Janina; Król, Karolina; Krawczyńska, Agata; Antushevich, Hanna; Pawlina, Bartosz; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota. Interleukin-1β modulates melatonin secretion in ovine pineal gland: ex vivo study. BioMed Research International 2015, 2015: art. no 526464 (1-10) (doi: 10.1155/2015/526464).
  3. Herman, Andrzej P.; Herman, Anna; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Tomaszewska-Zaremba, Dorota. Caffeine stimulates in vitro pituitary LH secretion in lipopolysaccharide-treated ewes. Reproductive Biology 2015, 15(1): 20-26 (doi: 10.1016/j.repbio.2014.12.001).
  4. Koziorowska-Gilun, Magdalena; Fraser, Leyland; Gilun, Przemysław D.; Koziorowski, Marek; Kordan, Władysław. Activity of antioxidant enzymes and their mRNA expression in different reproductive tract tissues of the male roe deer (Capreolus capreolus) during the pre-rut and rut seasons. Small Ruminant Research 2015, 129: 97-103 (doi: 10.1016/j.smallrumres.2015.05.006).
  5. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. New facts and the concept of physiological regulation of the dopaminergic system function and its disorders. Journal of Physiology and Pharmacology 2015, 66(3): 331-341 (pdf).
  6. Romerowicz-Misielak, Maria; Oren, Dan A.; Sowa-Kućma, Magdalena; Tabęcka-Łonczyńska, Anna; Gilun, Przemysław; Stefańczyk-Krzymowska, Stanisława; Koziorowski, Marek. Changes in melatonin synthesis parameters after carbon monoxide concentration increase in the cavernous sinus. Journal of Physiology and Pharmacology 2015, 66(4): 505-514 (pdf).
  7. Skipor, Janina; Szczepkowska, Aleksandra; Kowalewska, Marta; Herman, Andrzej P.; Lisiewski, Paweł. Profile of toll-like receptor mRNA expression in the choroid plexus in adult ewes. Acta Veterinaria Hungarica 2015, 63(1): 69-78 (doi: 10.1556/AVet.2014.027).
  8. Teixeira-Gomes, Ana-Paul; Harichaux, Gregoire; Gennetay, Dominique; Skipor, Janina; Thiery, Jean-Claude; Labas, Valerie; Dufourny, Laurence. Photoperiod affects the cerebrospinal fluid proteome: a comparison between short day- and long day-treated ewes. Domestic Animal Endocrinology 2015, 53: 1-8 (doi: 10.1016/j.domaniend.2015.04.003).
  9. Wiczkowski, Wiesław; Skipor, Janina; Misztal, Tomasz; Szawara-Nowak, Dorota; Topolska, Joanna; Piskuła, Mariusz K. Quercetin and isorhamnetin aglycones are the main metabolites of dietary quercetin in cerebrospinal fluid. Molecular Nutrition & Food Research 2015, 59(6): 1088-1094 (doi: 10.1002/mnfr.201400567).

2014

  1. Jablońska, Olga; Piasecka-Srader, Joanna; Nynca, Anna; Kołomycka, Agnieszka; Robak, Anna; Wąsowska, Barbara; Ciereszko, Renata E. 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters steroid secretion but does not affect cell viability and the incidence of apoptosis in porcine luteinized granulosa cells. Acta Veterinaria Hungarica 2014, 62(3): 408-421 (doi: 10.1556/AVet.2014.015).
  2. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława; Muszak, Jolanta; Gilun, Przemysław D.; Koziorowski, Marek. Cavernous sinus and its mysterious physiological functions: facts and hypotheses. Acta Biologica Cracoviensia Series Zoologia 2014, 55-56: 7-15 (pdf).
  3. Muszak, Jolanta; Krzymowski, Tadeusz; Gilun, Przemysław; Stefańczyk-Krzymowska, Stanisława. Countercurrent transfer of dopamine from venous blood in the cavernous sinus to the arterial blood supplying the brain – the perfused rabbit head as an experimental model. Journal of Physiology and Pharmacology 2014, 65(5): 641-648 (pdf).
  4. Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Koziorowski, Marek. Local transfer of testosterone and aromatase activity in the spermatic cord in wild boar/pig hybrids in short-daylight and long-daylight periods. Reproductive Biology 2014, 14(4): 282-288 (doi: 10.1016/j.repbio.2014.06.004).

2013

  1. Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Romerowicz-Misielak, Maria; Tabęcka-Łonczyńska, Anna; Przekop, Franciszek; Kosiorowski, Marek. Carbon monoxide-mediated humoral pathway for the transmission of light signal to the hypothalamus. Journal of Physiology and Pharmacology 2013, 64(6): 761-772 (pdf).
  2. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Fraser, Leyland; Koziorowski, Marek; Stefańczyk-Krzymowska, Stanisława; Kordan, Władysław. Antioxidant enzyme activity and mRNA expression in reproductive tract of adult male european bison (Bison bonasus, Linnaeus 1758). Reproduction in Domestic Animals 2013, 48(1): 7-14 (doi: 10.1111/j.1439-0531.2012.02015.x).
  3. Muszak, Jolanta. The impact of selected vasoactive factors on vascular functions. Polish Annals of Medicine – Rocznik Medyczny 2013, 20(2): 149-153 (doi: 10.1016/j.poamed.2013.09.001).
  4. Nynca, Anna; Nynca, Joanna; Wąsowska, Barbara; Kolesarova, Adriana; Kołomycka, Agnieszka; Ciereszko, Renata E. Effects of the phytoestrogen, genistein, and protein tyrosine kinase inhibitor-dependent mechanisms on steroidogenesis and estrogen receptor expression in porcine granulosa cells of medium follicles. Domestic Animal Endocrinology 2013, 44(1): 10-18 (doi: 10.1016/j.domaniend.2012.07.002).
  5. Szczepkowska, Aleksandra; Lagaraine, Christine; Robert, Vincent; Dufourny, Laurence; Thiery, Jean-Claude; Skipor, Janina. Effect of a two-weeks treatment with low dose of 2,2’,4,4’,5,5’ hexachlorobiphenyl (PCB153) on tight junction proteins expression in ovine choroid plexus during long and short photoperiod. Neurotoxicology and Teratology 2013, 37: 63-67 (doi: 10.1016/j.ntt.2013.03.061).

2012

  1. Grzegorzewski, Waldemar J.; Muszak, Jolanta; Wąsowska, Barbara; Jana, Barbara; Stefańczyk-Krzymowska, Stanisława. The influence of steroids on noradrenaline-mediated contractile reactivity of the superficial nasal and facia veins in cycling gilts. Polish Journal of Veterinary Sciences 2012, 15(2): 297-304 (doi: 10.2478/v10181-011-0147-z).
  2. Koziorowski, Marek; Stefańczyk-Krzymowska, Stanisława; Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Kamiński, Marian. The gaseous messenger carbon monoxide is released from the eye into the ophthalmic venous blood depending on the intensity of sunlight. Journal of Biological Regulators and Homeostatic Agents 2012, 26(1): 111-118.
  3. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. Advances in understanding the physiological mechanism of maternal immune tolerance to the embryo. Reproductive Biology 2012, 12(3): 265-270 (doi: 10.1016/j.repbio.2012.10.004).
  4. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. Local retrograde and destination transfer of physiological regulators as an important regulatory system and its role. Facts and hypothesis. Journal of Physiology and Pharmacology 2012, 63(1): 3-16 (pdf).
  5. Skipor, Janina; Misztal, Tomasz; Piskuła, Mariusz K.; Wiczkowski, Wiesław; Thiery, Jean-Claude. Phytoestrogens and thyroid hormone levels in the cerebrospinal fluid of ewes fed red clover silage. Small Ruminant Research 2012, 102(2-3): 157-162 (doi: 10.1016/j.smallrumres.2011.07.012).
  6. Skipor, Janina; Młynarczuk, Jarosław; Szczepkowska, Aleksandra; Lagaraine, Christine; Grochowalski, Adam; Guillame, Daniel; Dufourny, Laurence; Thiery, Jean-Claude. Photoperiod modulates access of 2,2’,4,4’,5,5’-hexachlorobiphenyl (PCB153) to the brain and its effect on gonadotropin and thyroid hormones in adult ewes. Ecotoxicology and Environmental Safety 2012, 78: 336-343 (doi: 10.1016/j.ecoenv.2011.11.042).
  7. Stefańczyk-Krzymowska, Stanisława; Wąsowska, Barbara; Gilun, Przemysław D.; Muszak, Jolanta; Grzegorzewski, Waldemar J. Relationship between contractions of the uterus and concentration of PGF2α in uterine venous blood after luteolysis in gilts. Reproduction in Domestic Animals 2012, 47(1): 98-104 (doi: 10.1111/j.1439-0531.2011.01807.x).
  8. Szczepkowska, Aleksandra; Młynarczuk, Jarosław; Grochowalski, Adam; Dufourny, Laurence; Thiery, Jean-Claude; Skipor, Janina. Effect of two-week treatment with low dose of ortho-substituted polychlorinated biphenyls (PCB104) and PCR153) on VRGF-receptor system expression in the choroid plexus in adult ewes. Polish Journal of Veterinary Sciences 2012, 15(4): 621-628 (doi: 10.2478/v10181-012-0098-z).
  9. Szczepkowska, Aleksandra; Wąsowska, Barbara; Gilun, Przemysław D.; Lagaraine, Christine; Robert, Vincent; Dufourny, Laurence; Thiery, Jean-Claude; Skipor, Janina. Pattern of expression of vascular endothelial growth factor and its receptors in ovine choroid plexus during long and short photoperiod. Cell and Tissue Research 2012, 350(1): 157-166 (doi: 10.1007/s00441-012-1431-7).

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Foreign co-operation LPR

  1. Laboratory of Physiology of Reproduction and Behavior, French National Institute of Agricultural Research (INRA), French national Center for scientific Research (CNRS), Francois Rabelais University of Tours and National Studs, Nouzilly, France (Dr Jean-Claude THIERY and Dr Laurence DUFOURNY);
  2. Research Unit for Animal Life Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan (Prof. Noboru MANABE);
  3. Institute of Biophysics, Biological Research Centre Hungarian Academy of Sciences in Szeged, Hungary (Dr. Maria DELI);
  4. Evestra Inc., San Antonio, USA.

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Achievements LPR

Main achievements of the Department concern the explanation of physiological mechanisms of local and central regulations of the reproductive processes in females.

In central nervous system:

  • It was demonstrated the influence of the photoperiod on the expression of transthyretin (protein transporter of thyroid hormones across the barrier blood-cerebrospinal fluid) in ovine choroid plexus. For the first time, the concentration of thyroid hormones in ovine cerebrospinal fluid was estimated. The relationship between the concentration of these hormones in blood and cerebrospinal fluid nether in breeding season nor in anestrus was found.
  • It was demonstrated the influence of the photoperid on the expression of short form of leptin receptors (OB-Ra) in porcine choroid plexus.
  • It was discovered retrograde transfer of neurohormones (LH-RH, oxytocin, beta-endorfin) and destination transfer of steroid pheromones to the blood supplying the brain and hypophysis in the perihypophyseal vascular complex (carotid rete mirabile – venous cavernous sinus).
  • It was revealed the humoral pathway for local transfer of boar pheromone – androstenol from the nasal mucosa to the blood supplying the brain and hypophysis and selective accumulation of androstenol in the hypophysis and certain structures of the hypothalamus and limbic system. We demonstrated that intramuscular application of androstenol accelerated the attainment of sexual maturation and influenced hormonal regulation of the periestrous period in gilts with experimental hypoosmia. The humoral pathway for boar pheromone action include their influence on the function of the superficial veins of the nose and face, regulating blood flow from the nasal cavity to the venous cavernous sinus of the perihypophyseal vascular complex.

In reproductive organs:

  • It was demonstrated that local transfer of steroid ovarian hormones from the ovarian blood and lymph to the ovarian artery elevated their concentrations in blood supplying the ovary by 80-150% and supplying the uterus and oviduct by 22-60%.
  • It was demonstrated a short regulatory loop of positive feedback between progesterone being retrograde transferred and its own secretion; positive effect of this transfer on activin A and androstenedione secretion, and negative effect on estradiol secretion.
  • It was demonstrated that in the mechanism of the retrograde and local destination transfer of uterine prostaglandins F2alfa and E2 a fundamental role fulfilled lymphatic vessels and small blood vessels of the mesometrium. Local transfer of prostaglandins is involved to the regulation of secretory function of the ovary as well as the blood supply to the reproductive organ and by this way it is one of main regulator of their cyclic function.
  • It was demonstrated the sensitivity of uterine and ovarian blood vessels to prostaglandins; in case of E2 it was dependent on the phase of the estrous cycle and pregnancy, and F2alfa – independent on the stage of reproductive processes.
  • It was demonstrated that infusion of oxytocin into the uterine artery on day 17 of the estrous cycle produced contractions of the uterus accompanied with immediate increase in PGF2alfa concentration in ovarian venous blood. Thus, pulsate elevation of PGF2alfa after luteolysis, when the endometrium undergoes of cyclic remodeling and the expression of PGF2alfa synthase is reduced, results from their excretion, together with uterine venous blood and lymph.

Original theory on mechanisms regulating the estrous cycle, with special regard of physiological ishemia of the endometrium effect on course of animal estrous cycle was published.

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Current projects LPR

  1. The level of inducible subunit – HIF-1α of the Hipoxia Inducible Factor-1 in nuclear fraction of the porcine endometrium in the estrous cycle (Statutory Found 2013; principal investigator: S. Stefańczyk-Krzymowska);
  2. Transport of dopamine along  the cranial nerves (III-VI) from the midbrain into venous blood of the cavernous sinus and its countercurrent transfer from the venous blood to the arterial blood supplying the brain in the rabbit (Statutory Found 2013; principal investigator: J. Muszak);
  3. The energy status of the pig uterus in conditions of its variable blood supply (Project of National Science Centre 2011-2014; principal investigator: B. Wąsowska);
  4. Effect of melatonin on choroid plexus response to immune stress in ewes (Project of National Science Centre OPUS 2012-2015; principal investigator: J. Skipor);
  5. Effect of photoperiod and melatonin on aquaporines expression in the choroid plexus of brain ventricles in ewes (Project of National Science Centre PRELUDIUM 2013-2015; principal investigator: A. Szczepkowska);
  6. Hypothalamic control of reproduction in sheep: seasonal modulation of proteins in CSF and choroid plexus (Polish –French project in the frame of POLONIUM 2013-2014, project coordinator  J. Skipor);
  7. Localization and correlation of apoptotic, anti-apoptotic factors and antioxidative enzymes in the porcine uterus under normoxia and hypoxia condition in vivo (Bilateral Scientist Exchange Program between Polish Academy of Sciences and Japan Society for the Promotion of Sciences 2013;  project coordinator: B. Wąsowska);
  8. Evaluation of safety and pro-health activity of home and commercial probiotic preparations: model research on animals with induced colitis” (Project of National Science Centre 2011-2014; principal investigator: E. Wasilewska; co-investigator: B. Wąsowska);
  9. The effect of immune stress and interleukin-1b on melatonin secretion in ewes during short and long days – in vitro and in vivo studies (Project of National Science Centre SONATA BIS 2012-2015: principal investigator: A. P. Herman, The Kielanowski Institute of Animal Physiology and Nutrition of Polish Academy of Sciences, co-investigator: A. Szczepkowska, P. Gilun).

Projects completed

  1. The contribution of the photoperiod and xenoestrogens in the modulation of the access of substances to the brain. (Polish-French Project, MSHE – INRA, head – Dr. Janina Skipor);
  2. Whether the information on the changes in natural illumination may be transferred by humoral pathway (humoral phototransduction) from the retina to the central nervous system? (project Ministry of Science and Higher Education, head – Prof. Marek Koziorowski).

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