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Methods


In vivo – skin wound healing models

transgenic
Foxn1::Egfp mice


Foxn1-/- Foxn1+/- Foxn1+/+

Foxn1−/−
 nude mice; CBy.Cg-Foxn1 /cmdb

Foxn1+/+ 
Balb/c/cmdb and C57BL (B6)

human

pigs

In vitro

primary cell cultures


keratinocytes
dermal fibroblast (DF)
adipose derived stem cells (ASC)
ear mesenchymal stem cells (EMSC)

co-culture model

vector transduction


adenoviruses
adeno-associated viruses
lentiviruses

skin explants

migration

gel contraction

tube formation

differentiation

Methods of skin samples analyses

qPCR

proteomic

flow cytometry

ELISA

western blot

lipid extraction

immunofluorescence

immunohistochemistry

Masson’s Trichrome