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Co-operation RI&P

International cooperation

  1. Lab. Reprod. Endocrinology, Graduate School of Natural Science and Technology, Okayama Univ., Japan (Prof. K. Okuda)
  2. Department Physiology and Structure, C.I.I.S.A., Faculdade de Medicina Veterinaria, Lisboa, Portugal (Prof. G.M. Ferreira-Diaz)
  3. Department of Obstetrics and Gynecology, C.I.I.S.A., Faculty of Veterinary Medicine, Technical University in Lisbon, Portugal (Prof. L.T. da Silva).
  4. Department of Dairy and Animal Science, Center of Reproductive Biology and Health, Pennsylvania University, Pennsylvania, USA ( Prof. J.L. Pate, Prof. T. Ott).
  5. Department of Animal and Range Sciences, North Dakota State University, USA (Prof. A. Grazul-Bilska).

Native cooperation

  1. Faculty of Pathology and Pharmacology, Department of Veterinary Medicine UW-M in Olsztyn, (dr hab. J. Jaroszewski, prof. UWM).
  2. Department of Medical Immunology, Laboratory of Reproductive Immunology, Institute of Immunology and Experimental Therapy PAN in Wrocław, (doc. A Chełmońska-Soyta).
  3. Department of Sheep and Goat Breeding, Faculty of Animal Breeding and Biology, Agricultural University of Cracow, (dr. hab. D. Zięba, prof. UP.).
  4. Faculty of Clinical Sciences (Animal Reproduction Team), Department of Veterinary Medicine UW-M in Olsztyn, (prof. T. Janowski, dr W. Barański, dr A. Rapacz-Leonard).
  5. Faculty of Functional Morphology, (Clinical Physiology Team), Department of Veterinary Medicine UW-M in Olsztyn, (dr hab. J. Całka, prof. UWM).
  6. Faculty of Animal Reproduction with Clinic, The Faculty of Veterinary Medicine, Wrocław University of Environmental and life Sciences (dr R. Kozdrowski,).

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The most important achievements of science RI&P

in Physiology of Reproduction:

  • Nitric Oxide (NO) is the main auto/paracrine mediator of luteolytic actions of PGF2α and cytokines during corpus luetum (CL) regression in the farm animals. Enzymes involved in NO production are expressed in the CL of mare and cow dependently on the phase of estrous cycle. Nitric oxide inhibits progesterone (P4) secretion, stimulates prostaglandin secretion and induces CL cells apoptosis during luteolysis.

  • The expression of tumor necrosis factor-α (TNFα) and its receptors in feline, bovine and equine uterus is dependent on the phase of estrous cycle and regulate the secretory functions of ovary and uterus during the cycle and early pregnancy. TNF differently modulates PGF2α and PGE2 secretion in uterus and ovary dependently on the phase of the estrous cycle. Moreover, in the cow in vivo, low concentrations of TNFα causes luteolysis, but high doses intensify corpus luteum (CL) activity and prolong the CL lifespan.
  • We showed that non-specific mechanisms of innate immune system localized in the feline endometrium were not distinctively affected as a results of either 4 weeks or 4 to 12 months oral treatment with medroxyprogesterone acetate (MPA). The increasing concentrations of plasma prostacyclin (PGI2) as well as both prostaglandins (PGF2α and PGE2), were shown to be the valuable markers of endotoxemia and inflammation during pyometra in cats, much better than unaffected concentrations of leukotrienes (LTB4 and LTC4) or TNF-α. In the epithelial endometrial cells was present: lipopolisaccharide (LPS)-challenged TNF-α secretion, increased expressions of Toll-like receptors (TLRs) and TNF/TNFR1 as well as increased synthesis of prostaglandins. Based on our results, the endometrial epithelial, but not stromal cells, were identified as the main cells responsible for the initial defence against invading pathogens.
  • We confirmed that feline placenta poses an active molecular tools responsible for steroidobiogenesis. We also showed that concentrations of placental progesterone are mirrored by plasma progesterone in the course of pregnancy in the domestic cat. We confirmed the presence of StAR protein and 3-βHSD in the decidual cells of placenta. Besides of this, we showed that feline placenta is a source of PGF2α. This placental PGF2α is assumed to play a key role in luteolysis in the periparturient females, since the differences in the expressions of either intraluteal prostaglandins receptors or synthases between pregnant or pseudopregnant cats were not shown. The increased concentrations of either plasma PGFM or placental PGF2α were observed in the last week of pregnancy. Surprisingly, the protein and mRNA expression of PGFS (which presents 87% of homology with AKR1C3) and PGES was increased during first 2.5 to 3 weeks of pregnancy and gradually dropped down towards end of pregnancy. The pattern of PTGS2 expression was similar to the plasma concentration of PGFM and placental concentration of PGF2α.
  • Lysophosphatidic acid (LPA) plays auto-paracrine role in the bovine CL and uterus.  We demonstrated the LPA and LPARs are presence in bovine uterus and CL. Lysophosphatidic acid stimulates luteotropic PGE2 production in cultured stromal endometrial cells, however inhibits luteolytic PGF2α synthesis in epithelial cells. Additionally, LPA stimulates P4 secretion from luteal CL cells of the mid luteal phase.
  • Glikokortykosteroids (GCs) play role as local mediators of the bovine uterus functions modulating prostaglandins secretion. In early pregnancy cortisol can serve as a mediator of luteotropic action of INFτ stimulates P4 and PGE2 secretion supporting CL functions and the early pregnancy development.

  • Leukotrienes (LT) play roles in auto/paracrine mechanisms regulation ovaries and endometrium during the estrous cycle and early pregnancy in cows. Leukotrienes modulates prostaglandin secretion in CL and uterus during the estrous cycle and early pregnancy. Moreover, LT stimulates progesterone, 17β-estradiol and PGF2α secretion in follicular granulosa cells.

in Pathology of Reproduction:

  • It has been shown that neutrophils (PMN) and extracellular network neutrophils (NETs), induced during persistent post-insemination endometritis can be the cause of dysfunction of secretory and structural endometrial fuctiona and are involved in the pathogenesis of endometrial fibrosis in mares (degenerative persisyen endometritis; endometrosis). In response to infectious agents, neutrophils form NETs, which additionaly to protection against the spread of pathogens, may also be responsible for the anti-inflammatory response, kill bacteria, but also cause tissue damage. NETs elements (ELA, CAT, CTGF, MPO), prostaglandins and cytokines are involved in pathogenesis of endometrial fibrosis in mares (endometrosis).
  • The expression of PG synthases, interleukin and their receptors changed in the course of endometriosis in the mare. There are endometrial secretory changes in the course of this disease. The secretion of PG in response to factors alters depending the endometrosis stage.
  • We proposed the diagnostic schemes for the sub-fertile mares, including mares that do not become pregnant, loss embryo or miscarriaged. Our studies showed: i) that either cytologic smears performed by usage of a cytobrush or endometrial biopsies have a good predictive value for diagnosing of endometritis; ii) the presence of neutrophils in the cytological smear is a good predictive marker of inflammatory conditions in equine uterus. The presence of at least 2% of neutrophils in endometrium is a valuable and predictive marker of endometritis; iii) during subclinical endometritis, dependently on the severity of inflammation connects with the neutrophils influx, the changes in the expression of: Toll-like receptors 2 and 4, TNF-α, IL-1β and IL-6 are observed. In the course of endometritis, the fluctuations of the secretory functions, including synthesis and secretion of the prostaglandins, prostacyclin and leukotrienes are observed.
  • In the studies concerning the participation of arachidonic acid (AA) metabolites in contractility of the inflamed porcine uterus it was revealed that PGF2α, PGI2, LTC4 and LTD4 increase, while PGE2 decreases the contraction intensity and frequency acting via subtype of receptors – EP2 and EP4. Moreover, we found that PGF2α and PGI2  influence on myometrial contraction activity not only through their receptors but also through receptors for other prostanoids.
  • The inflammatory processes in the porcine and bovine endometrium lead to changes the secretion function of the reproductive tract. In the pig, LH, PRL and estrogens are decreasing and androstendion is rising under endometritis. In both species inflammation of endometrium causes severe disturbances in the AA metabolism and NO production. We demonstrated that during endometritis in pigs and cows change the secretion of PGE2, PGF2α and leukotriens(LT) B4 and LTC4, and expression of LT receptors (CysLT(1)R, Cyst(2)R, LTB4R1). It was also revealed that changes in the secretion of mentioned above factors and also PGI2 in the porcine inflamed uterus coincide with alterations in the expression and/or cellular localization of enzymes participating in their synthesis. Depending on the degree of changes in the secretion of PG and LT and their each other relation take place the different disturbances in the ovary function. The inflammatory processes caused by the bacteria in the endometrium and ovaries lead to the increase in NO production in these organs showing that NO participates in pathogenesis of the inflammation in porcine and bovine reproductive organs. The reduction in the number of nerve fibers in the vicinity of endometrial glands and blood vessels during endometritis induced by Escherichia coli infection in gilts was revealed.
  • In the porcine cystic ovaries, the significant increase in the density of noradrenergic nerve fibers and in the content of catecholamines is accompanied by changes in steroidogenic activity, suggesting that these substances, by influence on the steroidogenesis, might affect the course of this pathological state. The alterations in the noradrenergic innervation pattern and steroidogenic activity of the cystic ovaries are partly dependent on phase of the estrous cycle in which the induction of the ovarian cysts is initiated. Moreover, the increase in the number of noradrenergic nerve fibers in the cystic ovaries is connected with up-regulation in the nerve growth factor (NGF) expression. Contrary to noradrenergic fibers, there is a decrease in the number of cholinergic nerve fibers in the cystic ovaries in pigs. In the pathologically-changed ovaries also we found changes in the distribution and numbers of nerve processes expressing markers of sensory neurons (SP, CGRP, GAL). It appears possible that neuropeptides released from these terminals may take part in the etiopathogenesis of cystic ovarian disease.
  • Long-term E2 treatment (simulation of pathological states occurring with elevated estrogens levels) of adult gilts results to essential changes in the distribution and chemical coding of ovarian neurons in the dorsal root ganglia, caudal mesenteric ganglion, sympathetic chain ganglia, and in the paracervical ganglion, as well as in the population of intraovarian nerve fibers. Our findings suggest that hyperestrogenism caused by endogenous or exogenous estrogens, may regulate the transmission of sensory modalities from the ovary to the spinal cord, and gonadal function(s) by affecting ovary-projecting neurons.
  • Cytokine (TNFα, IL-1α), NO and bacterial endotoxin (LPS) participate in pathogenesis of inflammation of bovine mammary gland via stimulation of PG and LT. Glikokortykosteroids are local anti-inflammatory agents in bovine mammary gland: cortisol produced local in mammary gland inhibited stimulated cytokine PG secretion.
  • The isoflavones and their metabolites (phytoestrogens) affect on – disorder the secretory functions of uterus and CL in cows and mares during estrous cycle and early pregnancy.
  • Demonstration that hormonal manipulation – synchronization of ovulation, depends on method and kind of substance used (especially analogue of PGF2α) reduced CL sensitivity on endogenous luteotropic factors.

Fig. 1. Regulation of reproduction system function. Influence of cytokine (TNFα), nitric oxide (NO), ovarian steroids and phytoestrogens on arachidonic acid metabolism during luteolysis and early pregnancy in cattle.

Fig.2. Hypothetical model of the structural and functional regression of the bovine corpus luteum [Skarzyński et al. 2005, 2008, 2010; review papers].

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Mechanizmy Działania Hormonów

Hormonal Action Mechanisms

We study factors and mechanisms engaged in the proper course of reproductive processes.

Our research is dedicated to improve the knowledge about immune-endocrine, cellular and molecular regulations of uterine and ovarian functions during the estrous cycle and early pregnancy, mainly using the domestic pig model.

Factors and mechanisms engaged in embryo-maternal communication during the maternal recognition of pregnancy, conceptus implantation and placenta development are among our main scientific interests.

Early embryonic mortality, implantation failures and deficiencies in placenta development and function may result in decreased litter size or impaired offspring health. Our studies are dedicated to explain the causes of these disorders.

We are also studying the development of ovarian follicles and the quality of corpora lutea during physiological and disturbed reproductive processes.

An important aspect of our studies is to determine the effect of nutrition and various breeding procedures on the female reproductive success. We aim to identify markers of endometrial receptivity and placenta efficiency as potential indicators of maternal and fetal outcomes, which could have a practical application.

We are using both in vivo animal models and in vitro cell/tissue cultures to achieve our scientific goals. The use of cutting-edge molecular biology methods, microscopic techniques and functional analyses of living cells allow us to determine changes in mRNA and protein expression and the activity of intracellular signaling pathways.

Results of our studies may be helpful in elaborating new strategies to improve breeding performance of pigs, developing procedures to reduce prenatal mortality and to use livestock animals as models in biomedical research.

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Current projects RI&P

  • Immuno- and neuro-endocrine mechanisms of pathogenesis of female reproductive organs disorders: inflammation, degeneration, neoplasia ( PAS Statutory Fund).
  • Role of actin and its binding proteins in endometrial remodeling during early pregnancy in pigs (Special statutory fund;  2015-2017; B.M. Jalali).
  • Influence of Phor14-lytic peptide GnRH conjugated to limit the functionality of the gonadotropic cells in vitro and in vivo: potential biological effects and the possibility of developing a new method for the pharmacological control of animal reproduction laboratory rat model” (KNOW project, UMO-KNOW2016/IRZBiBŻ/PRO1/01/9, 2016-2019; M. Siemieniuch).
  • Role of leptin in the pathogenesis of the endometrium during obesity progression in mice (KNOW project, UMO-KNOW2016/IRZiBŻ/PRO1/01/3, 2016-2019; A.M. Galvao).
  • Fibrosis of the endometrium as a potential cause of disorders of implantation and early pregnancy loss in mares: effects of ovarian steroids, prostaglandins and lysophosphatidic acid and its receptors in the pathogenesis” (NSC MAESTRO Project Nr 2011/02/A/NZ5/00338; 2012-2017; D.J. Skarzynski).
  • The effect of prostaglandin F2α secretion and action of fibroblast growth factor in the corpus luteum cows involved in the regulation of angiogenesis and luteolysis (NSC OPUS project; 2011/03 / B / NZ9 / 01634; 2012-2016; D.J. Skarzynski).
  • The role myofibroblastów in chronic degenerative endometritis in mares (MS&HE Project, 0463 / IP1 / 2015/73; 2015-2018; A.Z. Szóstek).
  • The role of leptin in the pathogenesis of ovarian dysfunction in the development of obesity (Project NSC Sonata; 2014/15/D/CC4/01152; 2015-2018; A.M. Galvao).
  • The impact of the of transforming growth factor ß superfamily on the function of corpus luteum in mare (Higher Education Project; IP2014 01137; 2015-2017; A.M. Galvao).
  • Peroxisome proliferator-activated receptors (PPAR) – expression level and the factors that modify the intensity of the signal transduction in the uterus of cows during the estrous cycle and experimentally induced inflammatory processes (Project OPUS NCN; 2013/11/B/CC4/04516; 2014-2017; A. Korzekwa) .
  • Does the death of cells in the structural regression of the corpus luteum cows can proceed independently of caspase activation and induction of apoptosis? Project NSC Sonata N/2013/11/D/NZ /02685; 2014-2016; T. Hojo).
  • Expression profile of PPAR: PPARalfa, PPARbeta/delta and PPARgamma in the corpus luteum cows in the course of the estrous cycle and under the influence PGF2alfa (NSC Preludium project; 2014/15 /N/NZ9/ 02428; 2015-2017; B. Socha).
  • The role of vascular endothelial growth factor in angiogenesis and uterine adenomyosis the cows (Project NSC Preludium 2014/15/N/NZ9/02414; 2015-2017; M. Lupicka)

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Equipment and applied methods P&ToR

Preparation of the material for the studies:

  • The isolation of cells of corpus luteum, myometrium, endometrium and epithelium of oviduct
  • The cell cultures and strip incubation: an incubator (Memmert, Germany)

Analytical methods:

  • Viability of cells determination (cytotoxicity test): ELISA (Multiscan EX, Labsystems, Finland)
  • Enzyme immunoassay of steroids, protein hormones and prostaglandins (ELISA, Multiscan EX, Labsystems, Finland)
  • PCR and its modifications: RT-PCR, nested-PCR, touchdown PCR
  • RT PCR and Real-time PCR:
    • RNA isolation (spectrophotometer BioPhotometr, Eppendorf, and Nonodrop)
    • Reverse transcription, PCR (termocycler MJ Mini, BioRad)
    • Real-time PCR (access to: ABI Prism 7300, Applied Biosystems, USA)
    • Gel documentation system for electrophoresis gels (MiniBIS Pro, DNR Bio Imaging System, Israel)
  • 5’ and 3’ RACE PCR – Rapid Amplification of cDNA Ends PCR
  • Western Blot analysis (Mini PROTEAN 3 System)
  • Intracellular Ca2+ mobilization using Fura 2 (microscope Nikon Eclipse TS 100 NIS-Elements Basic Research software)
  • Immunohistochemistry (access to: microscope Imager Z1-Zeiss, Axio Vision 4.8 software)
  • Measurement of contractility of myometrial and oviductal strips: isometric contraction transducer (access to: the HSE Type 372, Hugo Sachs Elektronik, Germany)
  • Determination of catecholamine concentrations by HPLC with electrochemical detection (Hewlett-Packard, Series 1050)

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Publications P&ToR

2026

  1. Jana, Barbara orcid; Mówińska, Aleksandra orcid; Andronowska, Aneta orcid; Całka, Jarosław. Role of noradrenaline and adrenoreceptors in prostaglandin E2 generation in Escherichia coli lipopolysaccharide-treated porcine endometrial epithelial cells. Annals of Animal Science 2026, przyjęta do druku (10.2478/aoas-2025-0115).
  2. Serej, Oliwia orcid; Kowalik, Magdalena K. orcid; Rękawiecki, Robert orcid. DNA methylation in the ovary and uterus of mammalian animal models: implications for reproductive function. Genes 2026, 17(2): art. no 228 (1-16) (10.3390/genes17020228).

2025

  1. Jana, Barbara orcid; Andronowska, Aneta orcid; Całka, Jarosław; Mówińska, Aleksandra orcid. Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cells. Scientific Reports 2025, 15: art. no 2806 (1-11) (10.1038/s41598-025-86787-1).
  2. Jana, Barbara orcid; Całka, Jarosław; Mówińska, Aleksandra orcid. Noradrenaline and adrenoreceptors promote prostaglandin F2α generation in lipopolysaccharide-exposed endometrial epithelial cells of pigs (Sus scrofa domesticus). International Journal of Molecular Sciences 2025, 26(12): art. no 5874 (1-15) (10.3390/ijms26125874).
  3. Mówińska, Aleksandra orcid; Rękawiecki, Robert orcid; Zając, Paulina orcid; Kowalik, Magdalena K. orcid. Phoenixin-14 and its receptor GPR173 in the bovine endometrium: Expression and regulation during the estrous cycle and impact on secretory activity. Theriogenology 2025, 243: art. no 117456 (1-8) (10.1016/j.theriogenology.2025.117456).
  4. Neuman, Nicoletta M.; Gilun, Przemysław orcid; Koziorowska-Gilun, Magdalena; Janiszewski, Paweł; Dziekońska, Anna. Antioxidant Enzyme Activity and mRNA Expression in the Reproductive Tissues of Male European Red Deer (Cervus elaphus elaphus). International Journal of Molecular Sciences 2025, 26(15): art. no 7221 (1-14) (10.3390/ijms26157221).
  5. Rybska, Marta; Czyżewska-Dors, Ewelina; Wojciechowicz, Tatiana; Sowińska, Natalia; Łukomska, Anna; Augustyniak, Agata; Szyguła, Aleksandra; Gogulski, Maciej; Wąsowska, Barbara orcid; Skrzypski, Marek. Expression of adropin in the reproductive organs of healthy female dogs and those with cystic endometrial hyperplasia, pyometra, and ovarian cysts. Veterinary Journal 2025, 314: art. no 106478 (1-11) (10.1016/j.tvjl.2025.106478).
  6. Sienkiewicz-Szłapka, Edyta; Janysz, Arleta; Wróbel, Michał H. orcid. Quantification of lawsone and p-phenylenediamine in natural henna products for hair colouring available in poland. Contact Dermatitis 2025, 92(4): 261-272 (10.1111/cod.14739).
  7. Wróbel, Michał H. orcid. A comparative study of the effect of commonly used pesticides on cervical contractions in pregnant cows, in vitro. Toxics 2025, 13(9): art. no 793 (1-14) (10.3390/toxics13090793).

2024

  1. Celeiro e Silva, Beatriz; Drzewiecka, Ewa M. orcid; Piotrowska-Tomala, Katarzyna K. orcid; Alpoim-Moreira, Joana; Sadowska, Agnieszka orcid; Kowalik, Magdalena K. orcid; Pimenta, Jorge; Rebordão, Maria R.; Ferreira-Dias, Graça M.; Skarżyński, Dariusz J. orcid; Szóstek-Mioduchowska, Anna Z. orcid The alteration in myometrial mRNA transcription of the regulatory genes of DNA methylation in mare with endometrosis. Reproductive Biology 2024, 24(2): art. no 100962 (1-7) (10.1016/j.repbio.2024.100962).

  2. Herman, Andrzej P.; Tomczyk, Monika; Wójcik, Maciej; Bochenek, Joanna; Antushevich, Hanna; Herman, Anna; Wiechetek, Wiktoria; Szczepkowska, Aleksandra D. orcid; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota. Effect of caffeine on the inflammatory-dependent changes in the GnRH/LH secretion in a female sheep model. International Journal of Molecular Sciences 2024, 25(5): art. no 2663 (1-15) (10.3390/ijms25052663).

  3. Jana, Barbara orcid; Całka, Jarosław; Andronowska, Aneta orcid; Mówińska, Aleksandra orcid; Witek, Krzysztof J. orcid; Palus, Katarzyna. Noradrenaline and adrenoreceptors are involved in the regulation of prostaglandin I2 production in the porcine endometrium after experimentally induced inflammation. International Journal of Molecular Sciences 2024, 25(12): art. no 6313 (1-17) (10.3390/ijms25126313).

  4. Neuman, Nicoletta M.; Dziekońska, Anna; Orzołek, Aleksandra; Gilun, Przemysław D. orcid A comparison of the biological properties of European red deer (Cervus elaphus elaphus) spermatozoa stored in the epididymides and in a liquid state at 5 °C. Animal Reproduction Science 2024, 260: art. no 107387 (1-13) (10.1016/j.anireprosci.2023.107387).

  5. Piotrowska-Tomala, Katarzyna K. orcid; Szóstek-Mioduchowska, Anna Z. orcid; Drzewiecka, Ewa M. orcid; Jończyk, Agnieszka W. orcid; Wójtowicz, Anna orcid; Wróbel, Michał H. orcid; Ferreira-Dias, Graça M.; Skarżyński, Dariusz orcid. Prostaglandin pathways in equine myometrium regulations: endometrosis progression. Frontiers in Veterinary Science 2024, 11: art. no 1479508 (1-10) (10.3389/fvets.2024.1479508).

  6. Piotrowska-Tomala, Katarzyna K. orcid; Szóstek-Mioduchowska, Anna Z. orcid; Jończyk, Agnieszka W. orcid; Drzewiecka, Ewa M. orcid; Wróbel, Michał H. orcid; Hojo, Takuo; Ferreira-Dias, Graça M.; Skarżyński, Dariusz J. orcid The effect of lysophosphatidic acid on myometrial contractility and the mRNA transcription of its receptors in the myometrium at different stages of endometrosis in mares. BMC Veterinary Research 2024, 20: art. no 571 (1-14) (10.1186/s12917-024-04384-2).

  7. Rybska,Marta; Skrzypski, Marek; Billert, Maria; Wojciechowicz, Tatiana; Łukomska, Anna; Pawlak, Piotr; Nowak, Tomasz; Pusiak, Karolina; Wąsowska, Barbara orcid. Nesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14. BMC Veterinary Research 2024, 20: art. no 486 (1-11) (10.1186/s12917-024-04336-w).

  8. Wojciechowska, Anna M. orcid; Zając, Paulina orcid; Gogola-Mruk, Justyna; Kowalik, Magdalena K. orcid; Ptak, Anna . Myo-inositol and D-chiro-inositol reduce DHT-stimulated changes in the steroidogenic activity of adult granulosa cell tumors. International Journal of Molecular Sciences 2024, 25(20): art. no 10974 (1-12) (10.3390/ijms252010974).

  9. Wróbel, Michał H. orcid; Młynarczuk, Jarosław J. orcid; Rękawiecki, Robert orcid. Effects of commonly used carbamates (carbaryl and thiram) on the regulatory, secretory and motor functions of bovine cervixes in vitro. Theriogenology 2024, 218: 183-192 (10.1016/j.theriogenology.2024.01.030).

2023

  1. Fabová, Zuzana; Tarko, Adam; Mlynček, Miloš; Kotwica, Jan; Sirotkin, Aleksandrer V. Flaxseed can Prevent Adverse Effects of Toluene on Human Ovarian Cell Functions. Current Bioactive Compounds 2023, 19(7): art. no e020123212322 (1-xx) (10.2174/1573407219666230102121435).

  2. Gilun, Przemysław; Koziorowska-Gilun, Magdalena; Wąsowska, Barbara; Sowa-Kućma, Magdalena; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Kordan, Władysław; Koziorowski, Marek. Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season. Polish Journal of Veterinary Sciences 2023, 26(1): 65-82 (10.24425/pjvs.2023.145008).

  3. Rękawiecki, Robert; Wróbel, Michał H.; Zając, Paulina; Serej, Oliwia; Kowalik, Magdalena K. Luteotropic and luteolytic factors modulate the expression of nuclear receptor coregulators in bovine luteal cells independently of histone acetyltransferase and histone deacetylase activities. Animals 2023, 13(17): art. no 2784 (1-16) (10.3390/ani13172784).

  4. Rybska, Marta; Billert, Maria; Skrzypski, Marek; Wojciechowicz, Tatiana; Kubiak, Magdalena; Łukomska, Anna; Nowak, Tomasz; Włodarek, Jan; Wąsowska, Barbara. Expression and localization of the neuropeptide phoenixin-14 and its receptor GRP173 in the canine reproductive organs and periovarian adipose tissue. Animal Reproduction Science 2023, 255: art. no 107282 (1-18) (10.1016/j.anireprosci.2023.107282).

  5. Sirotkin, Alexander V.; Macejková, Martina; Tarko, Adam; Fabova, Zuzana; Alwasel, Saleh; Kotwica, Jan; Harrath, Abdel H. Ginkgo, fennel, and flaxseed can affect hormone release by porcine ovarian cells and modulate the effect of toluene. Reproductive Biology 2023, 23(1): art. no 100736 (1-6) (10.1016/j.repbio.2023.100736).

  6. Sirotkin, Alexander V.; Tarko, Adam; Fabova, Zuzanna; Valocky, Igor; Alwasel, Saleh; Kotwica, Jan; Harrath, Abdel H. Can flaxseed, chia or puncture vine affect mare ovarian cell functions and prevent the toxic effect of the environmental contaminant toluene. Theriogenology 2023, 208: 178-184 (10.1016/j.theriogenology.2023.06.018).

  7. Sirotkin, Alexander V.; Tarko, Adam; Kotwica, Jan; Mlyncek, Milos; Alwasel, Saleh; Nitrayova, Sona; Harrath, Abdel H. Three medicinal plants affecting human ovarian cell viability, hormone release, and response to environmental contaminant toluene. Physiological Reports 2023, 72(2): 259-268 (10.33549/physiolres.934986).

  8. Szczepkowska, Aleksandra; Bochenek, Joanna; Wójcik, Maciej; Tomaszewska-Zaremba, Dorota; Antushevich, Hanna; Tomczyk, M.; Skipor, Janina; Herman, Andrzej P. Effect of caffeine on adenosine and ryanodine receptor gene expression in the hypothalamus, pituitary, and choroid plexus in ewes under basal and LPS challenge conditions. Journal of Animal and Feed Sciences 2023, 32(1): 17-25 (10.22358/jafs/156762/2022).

  9. Tarko, Adam; Štochmaľová, Aneta; Harrath, Abdel H.; Kotwica, Jan; Baláži, Andrej; Sirotkin, Alexander V. Quercetin can affect porcine ovarian cell functions and to mitigate some of the effects of the environmental contaminant toluene. Research in Veterinary Science 2023, 154: 89-96 (10.1016/j.rvsc.2022.12.005).

2022

  1. Dobrzyń, Karolina; Kowalik, Magdalena K. Molekularny mechanizm działania progesteronu w żeńskim układzie rozrodczym [Molecular mechanism of progesterone action in the female reproductive system]. Postępy Biochemii – Advances in Biochemistry 2022, 68(3): 336-343 (10.18388/pb.2021_458).

  2. Fabová, Zuzana; Tarko, Adam; Harrath, Abdel Halim; Alwasel, Saleh; Kotwica, Jan; Sirotkin, Alexander V. Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells. Reproduction in Domestic Animals 2022, 57(11): 1307-1318 (10.1111/rda.14204).

  3. Kowalik, Magdalena K.; Dobrzyń, Karolina; Młynarczuk, Jarosław; Rękawiecki, Robert. Effect of steroid hormones, prostaglandins (E2 and F2α), oxytocin, and tumor necrosis factor alpha on membrane progesterone (P4) receptors gene expression in bovine myometrial cells. Animals 2022, 12(4): art. no 519 (1-13) (10.3390/ani12040519).

  4. Mańkowska, Anna; Gilun, Przemysław D.; Zasiadczyk, Łukasz; Sobiech, Przemysław; Fraser, Leyland. Expression of TXNRD1, HSPA4L and ATP1B1 genes associated with the freezability of boar sperm. International Journal of Molecular Sciences 2022, 23(16): art. no 6320 (1-18) (10.3390/ijms23169320).

  5. Nowak, Sławomir; Gilun, Przemysław; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Koziorowska-Gilun, Magdalena; Wąsowska, Barbara. Carbon monoxide (CO) as a retinal regulator of heme oxygenases -1, and -2 (HO’s) expression. Biomedicines 2022, 10(2): art. no 358 (1-9) (10.3390/biomedicines10020358).

  6. Rybska, Marta; Billert, Maria; Skrzypski, Marek; Kubiak, Magdalena; Woźna-Wysocka, Magdalena; Łukomska, Anna; Nowak, Tomasz; Błaszczyk-Cichoszewska, Joanna; Pomorska-Móla, Małgorzata; Wąsowska, Barbara. Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression. Animal Reproduction Science 2022, 238: art. no 106931 (1-13) (10.1016/j.anireprosci.2022.106931).

  7. Sirotkin, Alexander V.; Tarko, Adam; Fabova, Zuzana; Mlynček, Miloš; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. Direct action of leptin, obestatin and ginkgo on hormone release by luteinised human ovarian granulosa cells [Erratum: Reproduction, Fertility and Development 2022, 34(18): 1128-1146, doi: 10.1071/RD22154_CO]. Reproduction, Fertility and Development 2022, 34(18): 1128-1134 (10.1071/RD22154).

  8. Wróbel, Michał H.; Młynarczuk, Jarosław; Kowalik, Magdalena K. Do cypermethrin and fenvalerate disturb the function of the bovine cervix in vitro. Journal of Endocrinology 2022, 253(3): 133-142 (10.1530/JOE-21-0336).

  9. Wróbel, Michał H.; Młynarczuk, Jarosław; Rękawiecki, Robert. Do commonly used herbicides (atrazine and glyphosate) have the potential to impair the contractions, prostaglandin releasing and conducting of oxytocin signal at the bovine cervix in vitro. Theriogenology 2022, 183: 26-35 (10.1016/j.theriogenology.2022.02.011).

2021

  1. Domżalska, Małgorzata; Wiczkowski, Wiesław; Szczepkowska, Aleksandra; Chojnowska, Sylwia; Misztal, Tomasz; Walter, Fruzsina R.; Deli, Maria A.; Ishikawa, Hiroshi; Schroten, Horst; Schwerk, Christian; Skipor, Janina. Effect of lipopolysaccharide-induced inflammatory challenge on β-glucuronidase activity and quercetin and its metabolites concentration in the choroid plexus, blood plasma and cerebrospinal fluid. International Journal of Molecular Sciences 2021, 22(13): art. no 7122 (1-17) (10.3390/ijms22137122).

  2. Gilun, Przemysław D.; Flisikowski, Krzysztof; Flisikowska, Tatiana; Kwiatkowska, Joanna; Wąsowska, Barbara; Koziorowska-Gilun, Magdalena. Role of methylation in period2 (PER2) transcription in the context of the presence or absence of light signals: natural and chemical – studies on the pig model. International Journal of Molecular Sciences 2021, 22(15): art. no 7796 (1-17) (10.3390/ijms22157796).

  3. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Dobrzyń, Karolina. The effect of DDE and dieldrin and their parental substances (DDT and aldrin) on PGE2 and PGF2α release from bovine endometrial explants collected during 120 to 180 days of the gestational period. Polish Journal of Veterinary Sciences 2021, 24(1): 5-12 (10.24425/pjvs.2020.135813).

  4. Płatosz, Natalia; Bączek, Natalia; Topolska, Joanna; Szawara-Nowak, Dorota; Skipor, Janina; Milewski, Stanisław; Wiczkowski, Wiesław. Chokeberry anthocyanins and their metabolites ability to cross the blood-cerebrospinal fluid barrier. Food Chemistry 2021, 346: art. no 128730 (1-11) (10.1016/j.foodchem.2020.128730).

  5. Rękawiecki, Robert; Dobrzyń, Karolina; Kowalik, Magdalena K. Steroid receptor coregulators can modulate the action of progesterone receptor during the estrous cycle in cow endometrium. Animals 2021, 11(11): art. no 3217 (1-14) (10.3390/ani11113217).

  6. Rybska, Marta; Woźna-Wysocka, Magdalena; Wąsowska, Barbara; Skrzypski, Marek; Kubiak, Magdalena; Błaszak, Beata; Łukomska, Anna; Nowak, Tomasz; Jaśkowski, Jędrzej M. Expression of transforming growth factor beta isoforms in canine endometrium with cystic endometrial hyperplasia–pyometra complex. Animals 2021, 11(3): art. no 1844 (1-14) (10.3390/ani11061844).

  7. Skipor, Janina; Szczepkowska, Aleksandra; Kowalewska, Marta; Domżalska, Małgorzata; Herman, Andrzej P.; Krawczyńska, Agata. Photoperiod alters the choroid plexus response to LPS-induced acute inflammation in ewes. Annals of Animal Science 2021, 21(2): 497-512 (10.2478/aoas-2020-0079).

  8. Szczepkowska, Aleksandra; Harazin, András; Barna, Lilla; Deli, Mária A.; Skipor, Janina. Identification of reference genes for circadian studies on brain microvessels and choroid plexus samples isolated from rats. Biomolecules 2021, 11(8): art. no 1227 (1-19) (10.3390/biom11081227).

  9. Szczepkowska, Aleksandra; Wójcik, Maciej; Tomaszewska-Zaremba, Dorota; Antushevich, Hanna; Krawczyńska, Agata; Wiechetek, Wiktoria; Skipor, Janina; Herman, Andrzej P. Acute effect of caffeine on the synthesis of pro-inflammatory cytokines in the hypothalamus and choroid plexus during endotoxin-induced inflammation in a female sheep model. International Journal of Molecular Sciences 2021, 22(24): art. no 13237 (1-13) (10.3390/ijms222413237).

  10. Wróbel, Michał H.; Młynarczuk, Jarosław. Chloroorganic (DDT) and organophosphate (malathion) insecticides impair the motor function of the bovine cervix. Toxicology and Applied Pharmacology 2021, 427: art. no 115667 (1-9) (10.1016/j.taap.2021.115667).

2020

  1. Fraser, Leyland; Paukszto, Łukasz; Mańkowska, Anna; Brym, Paweł; Gilun, Przemysław D.; Jastrzębski, Jan P.; Pareek, Chandra S.; Kumar, Dibyendu; Pierzchała, Mariusz. Regulatory potential of long non-coding RNAs (lncRNAs) in boar spermatozoa with good and poor freezability. Life-Basel 2020, 10(11): art. no 300 (1-26) (10.3390/life10110300).

  2. Młynarczuk, Jarosław J.; Górska, M.; Wróbel, Michał H. Effects of DDT, DDE, aldrin and dieldrin on prostaglandin, oxytocin and steroid hormone release from smooth chorion explants of cattle. Animal Reproduction Science 2020, 223: art. no 106623 (1-10) (10.1016/j.anireprosci.2020.106623).

  3. Rękawiecki, Robert; Dobrzyń, Karolina; Kotwica, Jan; Kowalik, Magdalena K. Progesterone receptor coregulators as factors supporting the function of the corpus luteum in cows. Genes 2020, 11(8): art. no 923 (1-12) (10.3390/genes11080923).

  4. Sirotkin, Alexander V.; Kádasi, Attila; Balaží, Andrey; Kotwica, Jan; Alrezaki, Abdulkarem; Harrath, Abdel H. Mechanisms of the direct effects of oil-related contaminants on ovarian cells. Environmental Science and Pollution Research 2020, 27: 5314-5322 (10.1007/s11356-019-07295-0).

  5. Sirotkin, Alexander V.; Kádasi, Attila; Balaží, Andrey; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. The action of benzene, resveratrol and their combination on ovarian cell hormone release. Folia Biologica 2020, 66(2): 67-71 (pdf).

  6. Sirotkin, Alexander V.; Tarko, Adam; Kotwica, Jan; Alrezaki, Abdulkarem; Harrath, Abdel H. Interrelationships between metabolic hormones, leptin and ghrelin, and oil-related contaminants in control of oxytocin and prostaglandin F release by feline ovaries. Reproductive Biology 2020, 20(2): 254-258 (10.1016/j.repbio.2020.02.003).

  7. Szczepkowska, Aleksandra; Kowalewska, Marta; Krawczyńska, Agata; Herman, Andrzej P.; Skipor, Janina. Photoperiod affects leptin action on the choroid plexus in ewes challenged with lipopolysaccharide – study on the mRNA level. International Journal of Molecular Sciences 2020, 21(20): art. no 7647 (1-16) (10.3390/ijms21207647).

  8. Tarko, Adam; Fabová, Z.; Kotwica, Jan; Valocký, I.; Alrezaki, Abdulkarem; Alwasel, Saleh; Harrath, Abdel H.; Sirotkin, Alexander V. The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel. General and Comparative Endocrinology 2020, 295: art. no 113491 (1-6) (10.1016/j.ygcen.2020.113491).

  9. Wróbel, Michał H.; Młynarczuk, Jarosław J. The effect of polychlorinated biphenyls (PCBs) on bovine oviductal contractions and LIF synthesis during estrous cycle, in vitro studies. Research in Veterinary Science 2020, 133: 188-193 (10.1016/j.rvsc.2020.09.027).

2019

  1. Herman, Andrzej P.; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Wojtulewicz, Karolina; Pawlina, Bartosz; Antushevich, Hanna; Herman, Anna; Tomaszewska-Zaremba, Dorota. Effect of central injection of neostigmine on the bacterial endotoxin induced suppression of GnRH/LH secretion in ewes during the follicular phase of the estrous cycle. International Journal of Molecular Sciences 2019, 20(18): art. no 4598 (1-19) (10.3390/ijms20184598).

  2. Kowalik, Magdalena K.; Dobrzyń, Karolina; Rękawiecki, Robert; Kotwica, Jan. Expression of membrane progestin receptors (mPRs) α, β and γ in the bovine uterus during the oestrous cycle and pregnancy. Theriogenology 2019, 140: 171-179 (10.1016/j.theriogenology.2019.08.028).

  3. Sirotkin, Alexander V.; Benčo, Andrej; Kotwica, Jan; Alwasel, Saleh H.; Harrat, Abdel H. Apoptosis signal-regulating kinase (ASK-1) controls ovarian cell functions. Reproduction, Fertility and Development 2019, 31(11): 1657-1664 (10.1071/RD19055).

  4. Sirotkin, Alexander V.; Benčo, Andrej; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. Apoptosis signal-regulating kinase controls porcine ovarian granulosa cell functions and their response to ghrelin. Journal of Biological Regulators and Homeostatic Agents 2019, 33(5): 1479-1483 (10.23812/19-96).

  5. Sirotkin, Alexander V.; Benčo, Andrej; Mlynček, Milos; Harrath, Abdel H.; Alwasel, Saleh; Kotwica, Jan. The involvement of the phosphorylatable and nonphosphorylatable transcription factor CREB-1 in the control of human ovarian cell functions. Comptes Rendus Biologies 2019, 342(3-4): 90-96 (10.1016/j.crvi.2019.03.002).

  6. Sirotkin, Alexander V.; Benčo, Andrej; Mlynček, Milos; Kotwica, Jan; Alwasel, Saleh; Harrath, Abdel H. Transcription factor p53 regulates healthy human ovarian cells function. Comptes Rendus Biologies 2019, 342(5-6): 186-191 (10.1016/j.crvi.2019.08.002).

  7. Szczepkowska, Aleksandra; Kowalewska, Marta; Skipor, Janina. Melatonin from slow-release implants upregulates claudin-2 in the ovine choroid plexus. Journal of Physiology and Pharmacology 2019, 70(2): 249-254 (10.26402/jpp.2019.2.08).

  8. Tarko, Adam; Štochmal’ová, Aneta; Jedličková, Katarína; Hrabovszká, Sandra; Vachanová, Adriana; Harrath, Abdel H.; Alwasel, Saleh; Alrezaki, Abdulkarem; Kotwica, Jan; Baláži, +; Sirotkin, Alexander V. Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release. Archives Animal Breeding 2019, 62: 345-351 (10.5194/aab-62-345-2019).

2018

  1. Herman, Andrzej P.; Tomaszewska-Zaremba, Dorota; Kowalewska, Marta; Szczepkowska, Aleksandra; Oleszkiewicz, Małgorzata; Krawczyńska, Agata; Wójcik, Maciej; Antushevich, Hanna; Skipor, Janina. Neostigmine attenuates proinflammatory cytokine expression in preoptic area but not choroid plexus during lipopolysaccharide-induced systemic inflammation. Mediators of Inflammation 2018, 2018: art. no 9150207 (1-9) (10.1155/2018/9150207).

  2. Kościukiewicz, Kinga; Wylot, Bartosz; Czelejewska, Wioleta; Gilun, Przemysław D.; Okrasa, Stanisław. The effects of mu-, delta- and kappa-opioid receptor activation on in vitro prolactin secretion by anterior pituitary cells of cyclic gilts. Journal of Animal and Feed Sciences 2018, 27(2): 114-122 (10.22358/jafs/91395/2018).

  3. Kowalik, Magdalena K.; Rękawiecki, Robert; Kotwica, Jan. Expression of membrane progestin receptors (mPRs) in the bovine corpus luteum during the estrous cycle and first trimester of pregnancy. Domestic Animal Endocrinology 2018, 63: 69-76 (10.1016/j.domaniend.2017.12.004).

  4. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Mietelska, Katarzyna; Kordan, Władysław. Determination of the activity and relative abundance of mRNA for antioxidant enzymes in stallion testicular and epididymal tissues: A comparison between two breeding seasons. Animal Reproduction Science 2018, 196: 230-238 (10.1016/j.anireprosci.2018.08.015).

  5. Rękawiecki, Robert; Kisielewska, Katarzyna; Kowalik, Magdalena K.; Kotwica, Jan. Methylation of progesterone receptor isoform A and B promoters in the reproductive system of cows. Reproduction, Fertility and Development 2018, 30(12): 1634-1642 (10.1071/RD17518).

  6. Szczepkowska, Aleksandra; Skowroński, Mariusz T.; Kowalewska, Marta; Milewski, Stanisław; Skipor, Janina. Effect of melatonin from slow-release implants on aquaporins (AQP1 and AQP4) in the ovine choroid plexus. Czech Journal of Animal Science 2018, 63(1): 32-42 (10.17221/6/2017-CJAS).

  7. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. The protein expression disorders of connexins (Cx26, Cx32 and Cx43)and keratin 8 in bovine placenta under the influence of DDT, DDE and PCBs. Polish Journal of Veterinary Sciences 2018, 21(4): 721-729 (10.24425/124311).

  8. Wróbel, Michał H. Glyphosate affects the secretion of regulators of uterine contractions in cows while it does not directly impair the motoric function of myometrium in vitro. Toxicology and Applied Pharmacology 2018, 349: 55-61 (10.1016/j.taap.2018.04.031).

  9. Wróbel, Michał H. Do chlorinated insecticides (aldrin and DDT) or products of their transformations (dieldrin and DDE) impair signal transfer from regulators (oxytocin and relaxin) of bovine myometrium motility in vitro? Environmental Research 2018, 167: 234-239 (10.1016/j.envres.2018.07.027).

  10. Wróbel, Michał H.; Młynarczuk, Jarosław J. Chlorinated insecticides (toxaphene and endrin) affect oxytocin, testosterone, oestradiol and prostaglandin secretion from ovarian and uterine cells as well as myometrial contractions in cow in vitro. Chemosphere 2018, 198: 432-441 (10.1016/j.chemosphere.2018.01.143).

2017

  1. Földešiová, Martina; Baláži, Andrej; Chrastinová, Lubica; Pivko, Juraj; Kotwica, Jan; Harrath, Abdel H.; Chrenek, Peter; Sirotkin, Alexander V. Yucca schidigera can promote rabbit growth, fecundity, affect the release of hormones in vivo and in vitro, induce pathological changes in liver, and reduce ovarian resistance to benzene. Animal Reproduction Science 2017, 183: 66-76 (10.1016/j.anireprosci.2017.06.001).

  2. Herman, Andrzej P.; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Wojtulewicz, Karolina; Antushevich, Hanna; Herman, Anna; Paczesna, Kamila; Romanowicz, Katarzyna; Tomaszewska-Zaremba, Dorota. Peripheral inhibitor of AChE, neostigmine, prevents the inflammatory dependent suppression of GnRH/LH secretion during the follicular phase of the estrous cycle. BioMed Research International 2017, 2017: art. no 6823209 (1-12) (10.1155/2017/6823209).

  3. Kowalewska, Marta; Herman, Andrzej P.; Szczepkowska, Aleksandra; Skipor, Janina. The effect of melatonin from slow-release implants on basic and TLR-4-mediated gene expression of inflammatory cytokines and their receptors in the choroid plexus in ewes. Research in Veterinary Science 2017, 113: 50-55 (10.1016/j.rvsc.2017.09.003).

  4. Kowalewska, Marta; Szczepkowska, Aleksandra; Herman, Andrzej P.; Pellicer-Rubio, Maria T.; Jałyński, Marek; Skipor, Janina. Melatonin from slow-release implants did not influence the gene expression of the lipopolysaccharide receptor complex in the choroid plexus of seasonally anoestrous adult ewes subjected or not to a systemic inflammatory stimulus. Small Ruminant Research 2017, 147: 1-7 (10.1016/j.smallrumres.2016.11.018).

  5. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Koziorowski, Marek; Kordan, Władysław. Expression of Hypoxia-Inducible Factor-1α and association with Cu/Zn superoxide dismutase in the reproductive tract tissues of adult male roe deer (Capreolus capreolus) during the reproduction season. Annals of Animal Science 2017, 17(4): 1063-1074 (10.1515/aoas-2017-0008).

  6. Kwiatkowska, Joanna; Wąsowska, Barbara; Gilun, Przemysław D. Expression of hypoxia inducible factor 1α and antioxidant enzymes: Superoxide dismutases-1 and -2 in ischemic porcine endometrium. Reproductive Biology 2017, 17(3): 289-293 (10.1016/j.repbio.2017.05.013).

  7. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Steroid hormone receptors in the corpus luteum. W: The life cycle of the Corpus Luteum. Ed.: Meidan, Rina. Springer International Publishing, Switzerland (doi: 10.1007/978-3-319-43238-0) 2017 (ISBN 9783319432366), Chapter 5: 79-98 (10.1007/978-3-319-43238-0_5).

  8. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. The expression of progesterone receptor coregulators mRNA and protein in corpus luteum and endometrium of cows during the estrous cycle. Animal Reproduction Science 2017, 183: 102-109 (10.1016/j.anireprosci.2017.05.011).

  9. Skipor, Janina; Kowalewska, Marta; Szczepkowska, Aleksandra; Majewska, Anna; Misztal, Tomasz; Jałyński, Marek; Herman, Andrzej P.; Ząbek, Katarzyna. Plasma and cerebrospinal fluid interleukin-1β during lipopolysaccharide-induced systemic inflammation in ewes implanted or not with slow-release melatonin. Journal of Animal Science and Biotechnology 2017, 8: art. no 76 (1-8) (10.1186/s40104-017-0206-0).

  10. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. Disorders in barrier protein mRNA expression and placenta secretory activity under the influence of polychlorinated biphenyls in vitro. Theriogenology 2017, 89: 9-19 (10.1016/j.theriogenology.2016.10.007).

  11. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. Changes in the mRNA expression of structural proteins, hormone synthesis and secretion from bovine placentome sections after DDT and DDE treatment. Toxicology 2017, 375: 1-9 (10.1016/j.tox.2016.11.016).

  12. Wróbel, Michał H. The preliminary study about the involvement of relaxine in adverse effect of polychlorinated biphenyls on bovine myometrial contractions. Journal of Clinical and Molecular Endocrinology 2017, 2(1): art. no 5 (1-6) (10.21767/2572-5432.10039).

  13. Wróbel, Michał H.; Młynarczuk, Jarosław J. Secretory function of ovarian cells and myometrial contractions in cow are affected by chlorinated insecticides (chlordane, heptachlor, mirex) in vitro. Toxicology and Applied Pharmacology 2017, 314: 63-71 (10.1016/j.taap.2016.11.011).

  14. Wróbel, Michał H.; Młynarczuk, Jarosław J. The inhibition of myometrial contractions by chlorinated herbicides (atrazine and linuron), and their disruptive effect on the secretory functions of uterine and ovarian cells in cow, in vitro. Pesticide Biochemistry and Physiology 2017, 142: 44-52 (10.1016/j.pestbp.2017.01.002).

2016

  1. Kowalik, Magdalena K.; Martyniak, Marcin; Rękawiecki, Robert; Kotwica, Jan. Expression and immunolocalization of membrane progesterone receptors in the bovine oviduct. Domestic Animal Endocrinology 2016, 55: 83-96 (10.1016/j.domaniend.2015.12.001).

  2. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium. Reproduction, Fertility and Development 2016, 28(7): 907-913 (10.1071/RD14325).

  3. Romerowicz-Misielak, Maria; Tabęcka-Łonczyńska, Anna; Kozioł, Katarzyna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Och, Waldemar; Koziorowski, Marek. Changes in gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor gene expression after an increase in carbon monoxide concentration in the cavernous sinus of male wild boar and pig crossbreed. Journal of Physiology and Pharmacology 2016, 67(3): 431-442 (pdf).

  4. Sirotkin, Alexander V.; Mertin, Dušan; Süvegová, Karin; Harrath, Abdel H.; Kotwica, Jan. Aging influences steroid hormone release by mink ovaries and their response to leptin and IGF-I. Biology Open 2016, 5(2): 174-177 (10.1242/bio.016436).

  5. Sirotkin, Alexander V.; Mertin, Dušan; Süvegová, Karina; Lauricik, Jozef; Morovic, Martin; Harrath, Abdel H.; Kotwica, Jan. Mink aging is associated with a reduction in ovarian hormone release and the response to FSH and ghrelin. Theriogenology 2016, 86(5): 1175-1181 (10.1016/j.theriogenology.2016.04.007).

  6. Szczepkowska, Aleksandra; Lagaraine, Christine; Robert, Vincent; Młynarczuk, Jarosław J.; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. PCB153 (2,2′,4,4′,5,5′-hexachlorobiphenyl) differentially affects the VEGF/VEGFR system depending on photoperiod in the ovine choroid plexus. Ecotoxicology and Environmental Safety 2016, 124: 303-308 (10.1016/j.ecoenv.2015.11.007).

2015

  1. Bochenek, Joanna; Skipor, Janina; Kowalewska, Marta; Herman, Andrzej P. The Toll-like receptors mRNA expression profile in the pineal gland of sheep during long and short days. Journal of Animal and Feed Sciences 2015, 24(3): 208-215 (10.22358/jafs/65626/2015).

  2. Cieślak, Magdalena; Chmielewska, Natalia; Romanowicz, Katarzyna; Młynarczuk, Jarosław J.; Wańkowska, Marta. Sex-dependent differences in the effect of early weaning on the chosen hormones secretion in sheep during the postnatal transition to puberty – preliminary results. Polish Journal of Veterinary Sciences 2015, 18(1): 71-78 (10.1515/pjvs-2015-0009).

  3. Herman, Andrzej P.; Bochenek, Joanna; Skipor, Janina; Król, Karolina; Krawczyńska, Agata; Antushevich, Hanna; Pawlina, Bartosz; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota. Interleukin-1β modulates melatonin secretion in ovine pineal gland: ex vivo study. BioMed Research International 2015, 2015: art. no 526464 (1-10) (10.1155/2015/526464).

  4. Herman, Andrzej P.; Herman, Anna; Skipor, Janina; Krawczyńska, Agata; Bochenek, Joanna; Tomaszewska-Zaremba, Dorota. Caffeine stimulates in vitro pituitary LH secretion in lipopolysaccharide-treated ewes. Reproductive Biology 2015, 15(1): 20-26 (10.1016/j.repbio.2014.12.001).

  5. Koziorowska-Gilun, Magdalena; Fraser, Leyland; Gilun, Przemysław D.; Koziorowski, Marek; Kordan, Władysław. Activity of antioxidant enzymes and their mRNA expression in different reproductive tract tissues of the male roe deer (Capreolus capreolus) during the pre-rut and rut seasons. Small Ruminant Research 2015, 129: 97-103 (10.1016/j.smallrumres.2015.05.006).

  6. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. New facts and the concept of physiological regulation of the dopaminergic system function and its disorders. Journal of Physiology and Pharmacology 2015, 66(3): 331-341 (pdf).

  7. Młynarczuk, Jarosław J.; Kotwica, Jan. Influence of 4-hydroxylated polychlorinated biphenyls on the secretory function of bovine ovarian cells: Role of the steroidogenic factor-1 receptor. Animal Reproduction Science 2015, 155: 89-98 (10.1016/j.anireprosci.2015.02.005).

  8. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Cloning and expression of progesterone receptor isoforms A and B in bovine corpus luteum. Reproduction, Fertility and Development 2015, 27(7): 1029-1037 (10.1071/RD13308).

  9. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Onapristone (ZK299) and mifepristone (RU486) regulate the messenger RNA and protein expression levels of the progesterone receptor isoforms A and B in the bovine endometrium. Theriogenology 2015, 84(3): 348-357 (10.1016/j.theriogenology.2015.03.024).

  10. Romerowicz-Misielak, Maria; Oren, Dan A.; Sowa-Kućma, Magdalena; Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Koziorowski, Marek. Changes in melatonin synthesis parameters after carbon monoxide concentration increase in the cavernous sinus. Journal of Physiology and Pharmacology 2015, 66(4): 505-514 (pdf).

  11. Wojciechowska, Anna M.; Młynarczuk, Jarosław J.; Kotwica, Jan. Short-term incubation of bovine placentome sections as a tool to study xenobiotic mechanism of action. Reproductive Biology 2015, 15(4): 238-246 (10.1016/j.repbio.2015.10.002).

  12. Skipor, Janina; Szczepkowska, Aleksandra; Kowalewska, Marta; Herman, Andrzej P.; Lisiewski, Paweł. Profile of toll-like receptor mRNA expression in the choroid plexus in adult ewes. Acta Veterinaria Hungarica 2015, 63(1): 69-78 (10.1556/AVet.2014.027).

  13. Teixeira-Gomes, Ana-Paul; Harichaux, Gregoire; Gennetay, Dominique; Skipor, Janina; Thiéry, Jean-Claude; Labas, Valerie; Dufourny, Laurence. Photoperiod affects the cerebrospinal fluid proteome: a comparison between short day- and long day-treated ewes. Domestic Animal Endocrinology 2015, 53: 1-8 (10.1016/j.domaniend.2015.04.003).

  14. Wiczkowski, Wiesław; Skipor, Janina; Misztal, Tomasz; Szawara-Nowak, Dorota; Topolska, Joanna; Piskuła, Mariusz K. . Quercetin and isorhamnetin aglycones are the main metabolites of dietary quercetin in cerebrospinal fluid. Molecular Nutrition & Food Research 2015, 59(6): 1088-1094 (10.1002/mnfr.201400567).

  15. Woźny, Maciej; Jakimiuk, Ewa; Brzuzan, Paweł; Florczyk, Maciej; Niewiadowska, Alicja; Obremski, Kazimierz; Gajęcka, Magdalena; Młynarczuk, Jarosław J.; Gajęcki, Maciej. Dichlorodiphenyldichloroethylene (DDE) residue limit exceeded in pig tissues after feed-borne exposure to maximum allowed concentration. Environmental Biotechnology 2015, 11(1): 1-9 (10.14799/ebms242).

  16. Wróbel, Michał H.; Grzeszczyk, Marlena; Młynarczuk, Jarosław J.; Kotwica, Jan. The adverse effects of aldrin and dieldrin on both myometrial contractions and the secretory functions of bovine ovaries and uterus in vitro. Toxicology and Applied Pharmacology 2015, 285(1): 23-31 (10.1016/j.taap.2015.03.005).

2014

  1. Cejko, Beata I.; Młynarczuk, Jarosław; Kowalski, Radosław K. Pozyskiwania nasienia karpia w warunkach kontrolowanych bez stosowania stymulacji hormonalnej. W: Szkolenie producentów ryb. Materiały szkoleniowe. Ed.: Ferlin, M. Polskie Towarzystwo Rybackie, Poznań 2014 (ISBN 9788393904518), 144-150.

  2. Jabłońska, Olga; Piasecka-Srader, Joanna; Nynca, Anna; Kołomycka, Agnieszka; Robak, Anna; Wąsowska, Barbara; Ciereszko, Renata E. 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters steroid secretion but does not affect cell viability and the incidence of apoptosis in porcine luteinized granulosa cells. Acta Veterinaria Hungarica 2014, 62(3): 408-421 (10.1556/AVet.2014.015).

  3. Kowalik, Magdalena K.; Rękawiecki, Robert; Kotwica, Jan. Expression and localization of progesterone receptor membrane component 1 and 2 and serpine mRNA binding protein 1 in the bovine corpus luteum during the estrous cycle and the first trimester of pregnancy. Theriogenology 2014, 82(8): 1086-1093 (10.1016/j.theriogenology.2014.07.021).

  4. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława; MusZak, Jolanta; Gilun, Przemysław D.; Koziorowski, Marek. Cavernous sinus and its mysterious physiological functions: facts and hypotheses. Acta Biologica Cracoviensia Series Zoologia 2014, 55-56: 7-15 (pdf).

  5. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Kotwica, Jan. The orphan nuclear receptor SF-1 is involved in the effect of PCBs, DDT, and DDE on the secretion of steroid hormones and oxytocin from bovine luteal cells during the estrous cycle in vitro. Theriogenology 2014, 81(7): 877-886 (10.1016/j.theriogenology.2014.01.016).

  6. Muszak, Jolanta; Krzymowski, Tadeusz; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława. Countercurrent transfer of dopamine from venous blood in the cavernous sinus to the arterial blood supplying the brain – the perfused rabbit head as an experimental model. Journal of Physiology and Pharmacology 2014, 65(5): 641-648 (pdf).

  7. Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Koziorowski, Marek. Local transfer of testosterone and aromatase activity in the spermatic cord in wild boar/pig hybrids in short-daylight and long-daylight periods. Reproductive Biology 2014, 14(4): 282-288 (10.1016/j.repbio.2014.06.004).

  8. Wróbel, Michał H.; Będziechowski, Paweł; Młynarczuk, Jarosław J.; Kotwica, Jan. Impairment of uterine smooth muscle contractions and prostaglandin secretion from cattle myometrium and corpus luteum in vitro is influenced by DDT, DDE and HCH. Environmental Research 2014, 132: 54-56 (10.1016/j.envres.2014.02.018).

2013

  1. Gilun, Przemysław D.; Stefańczyk-Krzymowska, Stanisława; Romerowicz-Misielak, Maria; Tabęcka-Łonczyńska, Anna; Przekop, Franciszek; Koziorowski, Marek. Carbon monoxide-mediated humoral pathway for the transmission of light signal to the hypothalamus. Journal of Physiology and Pharmacology 2013, 64(6): 761-772 (pdf).

  2. Kowalik, Magdalena K.; Rękawiecki, Robert; Kotwica, Jan. The putative roles of nuclear and membrane-bound progesterone receptors in the female reproductive tract. Reproductive Biology 2013, 13(4): 279-289 (10.1016/j.repbio.2013.09.001).

  3. Kowalik, Magdalena K.; Słonina, Dominika; Rękawiecki, Robert; Kotwica, Jan. Expression of progesterone receptor membrane component (PGRMC) 1 and 2, serpine mRNA binding protein 1 (SERBP1) and nuclear progesterone receptor (PGR) in the bovine endometrium during the estrous cycle and the first trimester of pregnancy. Reproductive Biology 2013, 13(1): 15-23 (10.1016/j.repbio.2013.01.170).

  4. Koziorowska-Gilun, Magdalena; Gilun, Przemysław D.; Fraser, Leyland; Koziorowski, Marek; Stefańczyk-Krzymowska, Stanisława; Kordan, Władysław. Antioxidant enzyme activity and mRNA expression in reproductive tract of adult male european bison (Bison bonasus, Linnaeus 1758). Reproduction in Domestic Animals 2013, 48(1): 7-14 (10.1111/j.1439-0531.2012.02015.x).

  5. Młynarczuk, Jarosław J.; Kowalik, Magdalena K. The effect of PCB126, 77, and 153 on the intracellular mobilization of Ca+2 in bovine granulosa and luteal cells after FSH and LH surge in vitro. Polish Journal of Veterinary Sciences 2013, 16(3): 417-424 (10.2478/pjvs-2013-0059).

  6. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Kotwica, Jan. Adverse influence of coumestrol on synthesis and secretion of oxytocin and progesterone from bovine luteal cells in the first trimester of pregnancy. Environmental Toxicology 2013, 28(7): 411-418 (10.1002/tox.20735).

  7. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Rękawiecki, Robert; Kotwica, Jan. The expression of Steroidogenic Factor-1 and its role in bovine steroidogenic ovarian cells during the estrus cycle and first trimester of pregnancy. Animal Reproduction Science 2013, 138(1-2): 74-81 (10.1016/j.anireprosci.2013.01.008).

  8. Młynarczuk, Jarosław J.; Wróbel, Michał H.; Ziółkowska, Anna; Kotwica, Jan. Involvement of the orphan nuclear receptor SF-1 in the effect of PCBs, DDT and DDE on the secretion of steroid hormones and oxytocin from bovine granulosa cells. Animal Reproduction Science 2013, 143(1-4): 30-37 (10.1016/j.anireprosci.2013.10.007).

  9. Muszak, Jolanta. The impact of selected vasoactive factors on vascular functions. Polish Annals of Medicine – Rocznik Medyczny 2013, 20(2): 149-153 (10.1016/j.poamed.2013.09.001).

  10. Nynca, Anna; Nynca, Joanna; Wąsowska, Barbara; Kolesarova, Adriana; Kołomycka, Agnieszka; Ciereszko, Renata E. Effects of the phytoestrogen, genistein, and protein tyrosine kinase inhibitor-dependent mechanisms on steroidogenesis and estrogen receptor expression in porcine granulosa cells of medium follicles. Domestic Animal Endocrinology 2013, 44(1): 10-18 (10.1016/j.domaniend.2012.07.002).

  11. Nynca, Anna; Słonina, Dominika; Jabłońska, Olga; Kamińska, Barbara; Ciereszko, Renata E. Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs. Acta Veterinaria Hungarica 2013, 61(1): 85-98 (10.1556/AVet.2012.060).

  12. Rękawiecki, Robert; Kowalik, Magdalena K.; Kotwica, Jan. Validation of housekeeping genes for studying differential gene expression in the bovine myometrium. Acta Veterinaria Hungarica 2013, 61(4): 505-516 (10.1556/AVet.2013.037).

  13. Szczepkowska, Aleksandra; Lagaraine, Christine; Robert, Vincent; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. Effect of a two-week treatment with low dose of 2,2’,4,4’,5,5’ hexachlorobiphenyl (PCB153) on tight junction proteins expression in ovine choroid plexus during long and short photoperiod. Neurotoxicology and Teratology 2013, 37: 63-67 (10.1016/j.ntt.2013.03.061).

  14. Ziółkowska, Anna; Młynarczuk, Jarosław J.; Kotwica, Jan. Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-α-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells. Polish Journal of Veterinary Sciences 2013, 16(2): 231-239 (10.2478/pjvs-2013-0033).

2012

  1. Grzegorzewski, Waldemar J.; MusZak, Jolanta; Wąsowska, Barbara; Jana, Barbara; Stefańczyk-Krzymowska, Stanisława. The influence of steroids on noradrenaline-mediated contractile reactivity of the superficial nasal and facia veins in cycling gilts. Polish Journal of Veterinary Sciences 2012, 15(2): 297-304 (10.2478/v10181-011-0147-z).

  2. Koziorowski, Marek; Stefańczyk-Krzymowska, Stanisława; Tabęcka-Łonczyńska, Anna; Gilun, Przemysław D.; Kamiński, Marian. The gaseous messenger carbon monoxide is released from the eye into the ophthalmic venous blood depending on the intensity of sunlight. Journal of Biological Regulators and Homeostatic Agents 2012, 26(1): 111-118.

  3. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. Local retrograde and destination transfer of physiological regulators as an important regulatory system and its role. Facts and hypothesis. Journal of Physiology and Pharmacology 2012, 63(1): 3-16 (pdf).

  4. Krzymowski, Tadeusz; Stefańczyk-Krzymowska, Stanisława. Advances in understanding the physiological mechanism of maternal immune tolerance to the embryo. Reproductive Biology 2012, 12(3): 265-270 (10.1016/j.repbio.2012.10.004).

  5. Rękawiecki, Robert; Rutkowska, Joanna; Kotwica, Jan. Identification of optimal housekeeping genes for examination of gene expression in bovine corpus luteum. Reproductive Biology 2012, 12(4): 362-367 (10.1016/j.repbio.2012.10.010).

  6. Sirotkin, Alexander V.; Kadasi, Attila; Balazi, Andrey; Bakova, Zuzana; Harrath, Abdel H.; Makarevich, Alexander V.; Kolesarova, Adriana; Chrenek, Peter; Kotwica, Jan; Toth, Tomas. Influence of petrochemical industry environmental contaminants on animal ovarian cells. Journal of Microbiology; Biotechnology and Food Sciences 2012, 2(2): 517-525 (pdf).

  7. Skipor, Janina; Misztal, Tomasz; Piskuła, Mariusz K.; Wiczkowski, Wiesław; Thiéry, Jean-Claude. Phytoestrogens and thyroid hormone levels in the cerebrospinal fluid of ewes fed red clover silage. Small Ruminant Research 2012, 102(2-3): 157-162 (10.1016/j.smallrumres.2011.07.012).

  8. Skipor, Janina; Młynarczuk, Jarosław J.; Szczepkowska, Aleksandra; Lagaraine, Christine; Grochowalski, Adam; Guillame, Daniel; Dufourny, Laurence; Thiéry, Jean-Claude. Photoperiod modulates access of 2,2’,4,4’,5,5’-hexachlorobiphenyl (PCB153) to the brain and its effect on gonadotropin and thyroid hormones in adult ewes. Ecotoxicology and Environmental Safety 2012, 78: 336-343 (10.1016/j.ecoenv.2011.11.042).

  9. Słonina, Dominika; Kowalik, Magdalena K.; Kotwica, Jan. Expression of progesterone receptor membrane component 1, serpine mRNA binding protein 1 and nuclear progesterone receptor isoforms A and B in the bovine myometrium during estrous cycle and early pregnancy. Journal of Reproduction and Development 2012, 58(3): 288-294 (10.1262/jrd.11-052T).

  10. Stefańczyk-Krzymowska, Stanisława; Wąsowska, Barbara; Gilun, Przemysław D.; MusZak, Jolanta; Grzegorzewski, Waldemar J. Relationship between contractions of the uterus and concentration of PGF2α in uterine venous blood after luteolysis in gilts. Reproduction in Domestic Animals 2012, 47(1): 98-104 (10.1111/j.1439-0531.2011.01807.x).

  11. Szczepkowska, Aleksandra; Młynarczuk, Jarosław J.; Grochowalski, Adam; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. Effect of a two-week treatment with low dose of ortho-substituted polychlorinated biphenyls (PCB104) and PCR153) on VRGF-receptor system expression in the choroid plexus in adult ewes. Polish Journal of Veterinary Sciences 2012, 15(4): 621-628 (10.2478/v10181-012-0098-z).

  12. Szczepkowska, Aleksandra; Wąsowska, Barbara; Gilun, Przemysław D.; Lagaraine, Christine; Robert, Vincent; Dufourny, Laurence; Thiéry, Jean-Claude; Skipor, Janina. Pattern of expression of vascular endothelial growth factor and its receptors in ovine choroid plexus during long and short photoperiod. Cell and Tissue Research 2012, 350(1): 157-166 (10.1007/s00441-012-1431-7).

  13. Wróbel, Michał H.; Młynarczuk, Jarosław J.; Kotwica, Jan. The effect of DDT and its metabolite (DDE) on prostaglandin secretion from epithelial cells and on contractions of the smooth muscle of the bovine oviduct in vitro. Toxicology and Applied Pharmacology 2012, 259(2): 152-159 (10.1016/j.taap.2011.12.019).

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Foreign co-operation P&ToR

  1. Research Institute of Animal Production, Nitra, Slovak Republic, prof. Alexander V. Sirotkin
  2. Department of Animal Science, University of Kentucky, Lexington, USA, prof. William J. Silvia
  3. Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Rumunia, prof. Horia Cernescu

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Achievements P&ToR

In our studies, it has been demonstrated:

  • The importance of adrenergic stimulation in the corpus luteum function;
  • The mechanism of autoregulation of corpus luteum function ;
  • Non-genomic effect of steroids on the uterus and ovary function;
  • The disturbing effect of xenobiotics on the function of reproductive tract, through the genomic and non-genomic way.

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Current projects P&ToR

  1. J.Kotwica – Effect of organochlorine xenobiotics on state of maternal-foetal connections in pregnant cows.
  2. M.Kowalik – The role of steroid hormones and lutetropic and luteolytic factors in regulation of the expression and function of membrane progesterone receptors in bovine reproductive tract.

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Equipment and applied methods LPR

Applied methods

1. In vivo methods

  • measurement of blood flow in the uterine artery of the pig by an electric transducer using an electromagnetic flowmeter (RT500 Narcomatic) under  general anaesthesia
  • measurement of porcine uterine intraluminal pressure/indirect uterine contractile activity (Bridge Amp AD Instruments) under  general anaesthesia
  • biotechnology methods of reproduction in the sheep: synchronization of ovulation (intravaginal suppositories) and acceleration of the breeding season (melatonin implants) for experimental purposes
  • mid-ventral laparotomy of the pig and sheep: ovariectomy, hysterectomy, induction of ischemia in the uterus of the pig by ligation of the uterine artery, intravital collection of uterine samples, implantation of catheters to blood vessels of mesometrium for blood collection and/or infusion of biologically active substances e.g. hormones under  general anaesthesia
  • implantation of catheters into blood vessels of the head and neck in the pig, sheep and rabbit under  general anaesthesia
  • long-term collection of cerebrospinal fluid from the third brain ventricle in sheep
  • long-term blood collection from jugular veins of the pig and sheep
  • continuous monitoring of blood oxygen saturation, pulse rate data, carbon dioxide detection and visual respiration indicator using pulse oximeter with carbon dioxide detector (Nonin Medical Inc. 9847V) under general anaesthesia

2. Ex vivo methods

  • the study of the contractile activity of the superficial veins of the head in the pig (frontal, nasal and facial veins)
  • the study of the contractile activity of isolated arteries of the female reproductive tract in the pig (the uterine artery)
  • the study on the isolated head of the pig, sheep, rabbit perfused with autologous blood
  • the study on the isolated pig’s ovary and uterus, testicle of the pig and rat perfused with autologous blood

3. Tissue incubations

  • pig’s hypothalamus explants

4. Analytic methods

4.1  Molecular biology methods

Expression on the mRNA level: Real Time PCR (qPCR) or Real Time PCR with TaqMan probes (qPCR TaqMan) and on the protein level: Western blot analysis (WB):

  • factors associated with production of cerebrospinal fluid: VEGF and its receptors: Flt-1, Flk-1 and co-receptor NRP-1, aquaporin-1,  aquaporin-4, claudin-2 (qPCR, WB) in the ovine choroid plexus
  • tight junction proteins restricting the passage of molecules via the intercellular path: transmembrane proteins: occludin, claudin-1, JAM-1 and cytosolic (scaffolding) proteins: ZO-1, ZO-2, ZO-3 and afadin-6 (qPCR, WB) in the ovine choroid plexus
  • transport proteins: transtyretin (TTR) in the ovine choroid plexus (qPCR)
  • lipopolysaccharide receptor complex: toll-like receptor 4 (TLR4), CD14 and MD-2 in the ovine choroid plexus (qPCR)
  • cytokines and their receptors and chemokines: TNFα, IL-1β, IL-6, TNFRI, TNFRII, IL-1R1, IL-1R2, IL-6R, gp130, MCP-1 in ovine choroid plexus (qPCR)
  • metalloproteinases and their tissue inhibitors: MMP2, MMP3, MMP9, TIMP-1 and TIMP-3 (qPCR) in the ovine choroid plexus
  • hypoxia marker HIF-1α in in the porcine uterus (qPCR, WB)
  • biological clock elements: Bmal 1, Clock, Npas2, Per 1-3, Cry 1-2 (qPCR TaqMan, WB) in the hypothalamus of domestic pig
  • nuclear receptors associated to the functioning of molecular biological clock: Rev-erb α, Rev-erb β, Ror α, Ror β, Ror γ (qPCR TaqMan) in the hypothalamus of domestic pig
  • antioxidant enzymes in the testis and epididymis of male deer and bison: EcSOD, GpX 4, GpX 5 (qPCR), SOD-1, Cat (qPCR, WB) and SOD-1, SOD-2 in porcine uterus (qPCR)
  • leptin receptor (Ob-Ra i Ob-Rb) in the choroid plexus and hypothalamus of domestic pig (qPCR, WB)

4.2 Immunoenzymatic (ELISA) method:

  • LPS Binding Protein in sheep
  • IL-6 in sheep

4.3 Radioimmunoassay (RIA) in plasma, tissues and body fluids:

  • thyroid hormones: T3 and T4 (free and total)
  • melatonin
  • hormones of reproductive system: estradiol, estrone, progesterone, testosterone, oxytocin, prostaglandin F2α
  • protein hormones: prolactin (PRL), luteinizing hormone (LH), follicle stimulating hormone (FSH), leptin, activin A in pig

4.4 Labeling hormones with radioisotope

  • oxytocin, GnRH, FSH, LH, prolactin, β-endorphin iodination (J125)

4.5 Immunohistochemical localization (with fluorochrome):

  • c-Fos protein (early response gene product) in porcine brain
  • NADPH-diaphorase (histochemical method) in the blood vessels of porcine brain
  • nitric oxide synthases, iNOS, eNOS in the porcine uterus and periovarian vascular complex and in rat’s large intestine
  • endogenous hypoxia marker (HIF-1α) in porcine oviduct and uterus
  • antioxidant enzymes SOD-1 and SOD-2 in porcine female reproductive tract and mice’s large intestine
  • Vascular Endothelial Growth Factor (VEGF) and its receptors Flt-1, Flk-1 in the ovine choroid plexus
  • antiapoptotic protein cFLIP in porcine female reproductive tract
  • apoptotic cells with TUNEL assay (Terminal Transferase dUTP Nick End Labeling) in porcine uterus and rat’s large intestine
  • dopamine hydroxylase (DβH) in porcine head vessels (facial, nasal and frontal vein) and peri-ovarian vascular complex
  • leptin receptor in porcine hypothalamus and choroid plexus
  • lipopolysaccharide receptors complex: CD14 and TLR4 in choroid plexus in sheep

4.6 Optical density measurments:

  • immunoreactivity measurement with Axio Vision Rel. 4.8.2 program

Equipment

1. Equipment for in vivo, ex vivo experiments:

  • Inhaled anesthetic apparatus for large animals (KOMESAROFF MINI-KOM)
  • Cardiomonitor (TEMED)
  • Pulse oximeter with carbon dioxide detector (Nonin Medica Inc.)
  • Set for experiments on isolated organs (UNIPER, Up-100, Hugo Sachs Elektronik-Harvard)
  • Set for measurement of isometric tension isolated blood vessels or muscle tissues (HSE F30, type 372, Hugo Sachs Elektronik-Harvard)
  • Infusion pumps
  • Peristaltic pump (Elphan, ZALIMP)
  • Electromagnetic transducers for blood flow measurement (Flowmeter Narcomatic RT500)
  • Surgical drill (Esculap)

2. Western blot and nucleic acids analysis workplace:

  1. Mini PROTEAN system with PowerPac basic power supply (electrophoresis system and wet transfer system, Bio Rad)
  2. Hematological roller (Chemland)
  3. Horizontal electrophoresis system Wide Mini Sub Cell (Bio Rad)
  4. Laminar flow chamber (Polon)

3. Equipment for Radioimmunoassay:

  • Gamma Counter (LKB)
  • Liquid Scintillation Counter (Beckman)
  • Radiometer with probe Rust-3

4. Basic lab equipment:

  • Vacuum pump BioVac 225 (OmegaLab)
  • Spectrophotometer (Beckman)
  • Stereoscopic microscope
  • Vortex (Biosan)
  • Shaker (Biosan)
  • Incubation chamber with shaker (Edmund Buehler)
  • Magnetic stirrer (Biosan, IKA WERKE)
  • Heated dry block  (OmegaLab)
  • Water bath
  • pH-meter (HANNA Instruments)
  • Mini centrifuge (Biosan, StarLab)
  • Centrifuge with cooling (MPW, Beckman)
  • Electronic scales (RADWAG, MEDICAT)
  • Ultra low freezer (SANYO)
  • Fume cupboard (Polon)
  • Ice maker (Scotsman)
  • Manifold Machine (pipettor) Hamilton Microlab 1000

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