In vivo – skin wound healing models
transgenic
Foxn1::Egfp mice

Foxn1-/- Foxn1+/- Foxn1+/+
Foxn1−/−
nude mice; CBy.Cg-Foxn1 /cmdb

Foxn1+/+
Balb/c/cmdb and C57BL (B6)

human

pigs

In vitro
primary cell cultures

keratinocytes
dermal fibroblast (DF)
adipose derived stem cells (ASC)
ear mesenchymal stem cells (EMSC)
co-culture model

vector transduction

adenoviruses
adeno-associated viruses
lentiviruses
skin explants

migration

gel contraction

tube formation

differentiation

Methods of skin samples analyses
qPCR

proteomic

flow cytometry

ELISA

western blot

lipid extraction

immunofluorescence

immunohistochemistry

Masson’s Trichrome
