Fish Proteomics course was held in the Institute’s Division of Reproductive Biology on 26-30 September 2016 within the framework of AQUAGAMETE Cost Action.
The Organizing Committee was comprised by the Staff of the Department of Gamete and Embryo Biology: Prof. Andrzej Ciereszko, Dr. Mariola Dietrich, Dr. Joanna Nynca, Dr. Mariola Słowińska, Agnieszka Mostek, MSc and Błażej Westfalewicz, MSc. In addition, the lectures were delivered by Prof. Serhiy Souchelnytskyi (College of Medicine, Qatar University) and Dr. Thomas Fröhlich (Laboratory for Functional Genome Analysis, Gene Center of the University of Munich).
The 7th Training School of the AQUAGAMETE COST Action, entitled “Fish proteomics” started on Monday September 26th. Organized by Professor Andrzej Ciereszko (Institute of Animal Reproduction and Food Research of Polish Academy of Sciences) it was run by the Training Team (Dr. Mariola Dietrich, Dr. Joanna Nynca, Dr. Mariola Słowińska, M.Sc. Agnieszka Mostek and M.Sc. Błażej Westfalewicz) and trainers: Professor Serhiy Souchelnytskyi from College of Medicine, Qatar University and Dr. Thomas Fröhlich from Laboratory for Functional Genome Analysis, Gene Center of the University of Munich. The training school was attended by 9 students from Brazil, China, Czech Republic, Hungary, Iran, Italy and Poland.
The Training School was organized in 9 lectures, covering important topics related to proteomics, including: Introduction to proteomics, Intact-protein proteomics by 2DE and MALDI mass spectrometry, Techniques of sample preparation for proteomic analysis, Linking genomics and proteomics, bioinformatics (Professor Serhiy Souchelnytskyi); 2D Difference gel electrophoresis (Błażej Westfalewicz); Proteomics in fish reproductive research (Professor Andrzej Ciereszko); Proteomic in Reproductive Science, Protein databases, and Quantitative proteomics (Dr. Thomas Fröhlich).
The core of the training school was extensive hands-on practical training supervised by the Training Team and Professor Serhiy Souchelnytskyi. On Monday 26th each student performed protein sample preparations from different fish tissues then started electrophoresis in the first dimension (isoelectrofocusing) which was run overnight. On Tuesday morning the isoelectrofocusing was finished and preparation SDS-gels for the second dimension was commenced. Electrophoresis in second dimension started in the evening and was run overnight. On Wednesday SDS-electrophoresis was finished, followed by fixation of gels and staining. Then samples for mass spectrometry analysis were prepared, including digestion of selected spots with trypsin. Next day tryptic digests were prepared and loaded on MALDI target plates. Next analytical trainings were performed in i) computer analysis of gels and ii) mass spectrometry identification of proteins. On the last day of the training school all activities were focused on reporting results, presentations of individual achievements by the participants and concluding seminar.
In summary, the students were exposed to new area of research in fish reproduction and successfully accomplished all planned objectives. Their active participation was also inspiration for Training Team and lecturers.
