Title:
„Unravelling the Mechanism of Formation and the Functional Significance of the Novel, Glioblastoma-Specific Localization Pattern of HCMV Protein”.
Place of employment:
Institute of Animal Reproduction and Food Research (IARFR)PAS, Department of Biology and Pathology of Human Reproduction Olsztyn, Poland.
Application:
Per e-mail to: a.andronowska@pan.olsztyn.pl
Deadline: 5 Sep 2023, 15:00 CET
Candidates need to apply to the Interdisciplinary Doctoral School of Agricultural Sciences of the IAR&FR for details and necessary forms please see.
Starting Date: Oct 2023
Length of scholarship: 48 months
Project Description:
Glioblastoma multiforme (GBM) is a malignant brain tumor. The prognosis for GBM patients after extensive treatment is very poor – 5 year survival rate is less than 10%. Additionally, the etiology and pathogenesis of this disease are still not understood. High percentage of glioblastomas were found to be positive for human cytomegalovirus (HCMV), which was found to be associated with worse prognosis for the patients. Adjuvant anti-HCMV treatments have shown increase in survival rates for GBM patients, confirming influence of HCMV infection on the outcome of the disease. The HCMV gene products were shown to influence glioblastoma proliferation and angiogenesis, induce apoptosis-resistance and immune evasion, suggesting an oncomodulatory role of HCMV in this disease. One of the HCMV proteins found in high percentage of glioblastomas is immediate early protein 1 (IE1). We detected a novel localization pattern of IE1 on mitotic chromosomes in glioblastoma cells. In addition to the known “chromosome painting” pattern we observed chromosome-associated spots (CAS) of IE1. This localization pattern was not present in other cell types of neural origin or other cells that support HCMV replication or latency, which are two known characteristics of the glioblastoma cells we used. We did, however detect it in another glioblastoma cell line, suggesting that the CAS localization of IE1 might be specific to glioblastoma. The goal of this project is to study chromatin association of HCMV IE1 protein in glioblastoma cells. Project will combine methods of virology, cell and molecular biology. We would like to study the possible function of the novel IE1 localization pattern. We believe that unravelling the function of IE1 CAS localization will not only further our understanding of the role that HCMV plays in glioblastoma, but may aid in future development of new therapeutic approaches targeting viral persistence.
TYPE of NCN grant: OPUS24 – NZ6.
Description of the tasks:
- Cloning of lentiviral vectors;
- Generation and validation of stable cell lines;
- Isolation of protein complexes;
- Quantitative MS analysis and processing and filtering of MS data.
Successful candidate will also be responsible for data analysis, writing publications and presenting results at seminars and conferences.
Qualifications:
- Masters in microbiology, biotechnology, biology or related discipline,
- Solid knowledge of molecular and cell biology techniques is required,
- Knowledge in the field of virology or microbiology and in vitro cultures is advantages,
- Ability to communicate easily in English,
- Ability to communicate well and work in a group.
Benefits:
- a stimulating research environment with motivated and interdisciplinary team in an international environment,
- an exciting project with cutting-edge technologies at the interface of basic research and translational medicine,
- personal and professional development opportunities – supported by internal and external training,
- the doctoral scholarship:PLN 4 200 gross.
Interested candidates are asked to provide the following documents:
- Motivation letter;
- CV including list of publications, conference presentations, technical expertise and awards;
- MS degree certificate, PhD diploma or certificate of participation in studies at the moment;
- Recommendation letter from at least two scientific mentors, confirming skills necessary for completing the project;
- Documents confirming proficiency in foreign languages;
- Other documents, that in the opinion of the candidate are important when considering him/her for the position;
- Signed statement: I hereby authorize you to process my personal data included in my job application for the needs of the recruitment process in accordance with the Personal Data Protection Act dated 29.08.1997 (uniform text: Journal of Laws of the Republic of Poland 2002 No 101, item 926 with further amendments).
Please direct any questions regarding the project to the PI: Dr hab. Magdalena Weidner-Glunde, email: m.weidner-glunde@pan.olsztyn.pl.
In your CV, please include a consent clause for the processing of personal data in the recruitment process:
„I consent to the processing of my personal data contained in the application documents by the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, 10-748 Olsztyn, ul. Tuwima 10, in order to carry out the recruitment process and publishing the full results of the competition on the Institute’s website.”
Information clause:
- The administrator of personal data processed as part of the recruitment process is the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, 10-748 Olsztyn, ul. Tuwima 10, phone no. 89 523 46 86, e-mail: instytut@pan.olsztyn.pl.
- Contact with the personal data protection officer is possible at the above-mentioned address.
- The provided personal data will be processed in order to carry out the current recruitment process and kept until its completion on the basis of expressed consent (in accordance with Article 6 (1) (a) of the GDPR).
- You have the right to withdraw consent at any time without affecting the lawfulness of the processing which was carried out on the basis of consent before its withdrawal.
- You have the right to access your personal data, request their rectification or removal. Submitting a request to delete data is tantamount to resignation from participation in the recruitment process. In addition, you have the right to request the restriction of processing in the cases specified in art. 18 GDPR.
- You have the right to lodge a complaint with the President of the Personal Data Protection Office against the unlawful processing of his personal data. This authority will be competent to consider the complaint, provided that the right to file a complaint concerns only the lawfulness of the processing of personal data, and not the recruitment process.
- Your data will not be profiled or made available to entities or third countries. The recipients of the data may be institutions authorized by law.
- Providing your personal data is not obligatory, but it is a necessary condition to participate in the recruitment process.
