Equipment and applied methods HAM

1. RIA and EIA of hormones and prostaglandins;
2. Isolation and in vitro culture of:
   • luminal and stromal cells of the porcine endometrium,
   • myometrial cells of the uterus,
   • endothelial cells,
   • epithelial cells of the oviduct,
   • luteal cells,
   • granulosa cells,
   • trophoblast cells,
   • anterior pituitary cells,
3. Collection, in vitro culture, cryopreservation and non-surgical transfer of porcine embryos;
4. Histological analysis of the female reproductive tract;
5. Immunohistochemical and immunofluorescent staining of tissues and cultured cells;
6. Scanning and transmission electron microscopy;
7. Western blot analysis;
8. Isolation of DNA / RNA;
9. Quantitative and qualitative DNA/ RNA analysis;
10. Real-time PCR;
11. Southern blot analysis;
12. Cell transfection with plasmid vectors.