We study the molecular and physiological processes that determine the quality and functionality of gametes across various animal species, including fish, birds, and mammals.
Our research interests center on the biology of gametes—sperm and oocytes.
We conduct studies on the processes of spermatogenesis, sperm maturation, capacitation (the process preparing sperm for fertilization), acrosomal reaction, and the key stages of fertilization. We also analyze post-translational modifications of gamete proteins such as phosphorylation, glycosylation, and redox changes, which play a significant role in regulating their functionality.
A key area of our research is investigating the functions of proteins such as secreted novel AID/APOBEC-like deaminase 1 (SNAD1) and the cysteine-rich soluble scavenger receptor (SSc5D). We explore molecular and cellular factors contributing to the occurrence of yellow semen syndrome in turkeys, considering the immune response and the semen microbiome.
Furthermore, we conduct experiments on peroxiredoxins (PRDX5 and PRDX6), studying their critical role in protecting sperm from oxidative stress. We examine mechanisms of sperm damage that occur during zootechnical procedures such as cryopreservation and semen sexing.
Our research employs advanced methods in transcriptomics, proteomics, microscopy, chromatography techniques, and flow cytometry combined with imaging. These tools allow us to conduct detailed analyses of gamete structure and function under both physiological and pathological conditions.
We are also developing methods for semen storage for both short- and long-term conditions. We are creating semen banks that are used in animal breeding programs and conservation efforts for endangered species.
Through this work, our research supports both practical breeding practices and efforts to preserve biodiversity.
