Ph.D. Opportunity in Molecular Biology: Exploring Epigenetic Memory and Immune Response to Vitamin D

Position Available: 1 Ph.D. Student in Molecular Biology

Research Focus: Epigenetic memory mechanisms in human immune cells’ response to vitamin D

Location: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

Application Deadline: November 24, 2024, 23:59 CET

Start Date: December 2, 2024

Duration: 4 years

Scholarship: 6,370 PLN gross/month (approx. 10% deductions)

Why Join Us?

We are seeking a motivated Ph.D. student to join our Nutrigenomics Team for a cutting-edge project led by Prof. Carsten Carlberg. You’ll work on understanding how vitamin D influences the “epigenetic memory” in immune cells – a frontier area with big potential for breakthroughs in health and nutrition.

This is a unique chance to:

  • Develop advanced skills in molecular biology and epigenetics.
  • Work hands-on with RNA-seq, ATAC-seq, and ChIPmentation to uncover cellular processes.
  • Collaborate with a vibrant, interdisciplinary research team.
  • Gain experience in a high-impact research project funded by the NCN OPUS grant.

About the Research Project

Your research will focus on:

  • Key techniques: Isolating immune cells, preparing RNA and chromatin libraries, and analyzing next-generation sequencing (NGS) data.
  • Proposed Ph.D. Thesis: The Role of the Vitamin D Receptor in Epigenetic Memory.
  • Goal: Reveal how vitamin D and its receptor influence long-term changes in immune cells.

What We’re Looking For

  • Education: Master’s degree in Molecular Biology, Biochemistry, Biotechnology, or a related field.
  • Skills: Experience in molecular techniques (PCR, RNA isolation, cell culture) and bioinformatics for high-throughput data.
  • Qualifications: Demonstrated research achievements (e.g., publications, awards, project involvement).
  • Soft Skills: Team player, proactive, well-organized, with excellent English communication skills.

Application Process

  1. Apply Online: Complete the application form here.
  2. Selection: Top candidates will be invited for an interview (in-person or online).
  3. Interview: Short presentation of your Master’s thesis and research interests.
  4. Notification: Results will be posted on our website within 10 days of the final decision.

Take the next step in your research career and join us on this exciting journey into molecular biology and epigenetics!

Apply now and be part of research that could shape the future of health sciences.

Good luck!

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Dr Radosław Kowalski inaugurates the Olsztyn Academy of Children

Dr Radosław Kowalski from the Gamete and Embryo Biology Team of our Institute gave a guest lecture at the inaugural meeting of the Olsztyn Academy of Children (OAD), which is run by the Planetarium in Olsztyn. This time the students were introduced to water, and more specifically to its superpowers as the source of life and health for all living organisms.

A scientist intrigued the youngest students with a lecture about humans and oceans. What do we have in common, why are humans aquatic mammals and why can we say that every human being is a living drop in the ocean? – asked Dr Radosław Kowalski during the inauguration of the OAD.

More information about the Olsztyn Children’s Academy can be found on the Planetarium website.

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Our scientists honoured by Society of Reproductive Biology

Our Institute is one of the leading scientific units for animal reproduction research in Poland. The accolades awarded to our scientists by the Society of Reproductive Biology confirm the top level of research carried out at the Institute.

The Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences – along with the Society of Reproductive Biology and the University of Warsaw – was the organiser of the 10th Congress of the Society of Reproductive Biology, held on 12-14 September in Warsaw. The conference brought together nearly 150 researchers from national and international scientific centres.

During the event, Dr Aneta Andronowska, Professor of IARFR PAS, from the Hormonal Action Mechanisms Team, received the Society’s highest accolade, the TBR medal, for her many years of organisational activity in support of the Society’s development.

Dr Maria M. Guzewska from the Hormonal Action Mechanisms Team of our Institute received the Professor Władysław Bielański Award, awarded by the Society once every four years. Dr Guzewska was recognised for her research on the role of embryonic signals in embryo-maternal communication involving extracellular vesicles.

Paulina Zając, our PhD student, was recognised for her poster entitled “Progestin and adipoQ receptor (PAQR) 7 and PAQR8 knockdown affects the function of bovine endometrial endothelial cells”, which she prepared under the supervision of Dr Magdalena Kowalik, head of the Physiology and Toxicology Team.

Congratulations!

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World Food Day 2024 – Let’s appreciate the beneficial role of microorganisms in food

Bacteria, yeasts and moulds are unlikely to have good PR, although many of them are useful and used in food production and preservation. After all, without them there is no yoghurt, cheese, pickles, bread, but also cocoa, tea and coffee. Our scientists are involved in research on checking the quality of these microorganisms and the safety of such foods, as well as looking for new strains.

16 October is World Food Day. On this occasion, Dr Anna Majkowska, head of the Microbiology Laboratory at our Institute, takes a closer look at the topic of microorganisms in food.

Although we usually associate micro-organisms in food with either being a potential threat to human health or contributing to food spoilage, many are essential in the production or preservation of food.

– An entire branch of the dairy industry is based on fermentation processes carried out by micro-organisms, with the production of cheeses, yoghurts, kefirs or various types of dairy drinks. Without bacteria, there would be no pickles, which have been around for centuries and are now becoming increasingly popular. Bread and cakes are made with baker’s yeast or sourdough starter containing bacteria and yeast. Cured meats such as salami are also made with bacteria. You probably don’t realise that thanks to fermentation we also have cocoa, coffee and tea. Not to mention a whole branch of wine, beer and spirit production – says Dr Anna Majkowska.

The researcher also recalls that bacteria have been used to preserve food or prepare fermented beverages from milk for centuries, although initially people did not know what was behind it. The conscious use of microorganisms only began with the groundbreaking research of the French chemist and microbiologist Louis Pasteur, who lived in the 19th century.

HOW DOES FERMENTATION WORK?

Bacteria ferment, i.e. break down sugars contained in vegetables, fruit or milk (here using lactose) and, on this basis, produce acids (e.g. lactic or acetic) and short-chain fatty acids. This lowers the pH level of the product in question – hence the sour taste of silage. As a result, the product is more difficult to access for undesirable bacteria, e.g. putrefactive bacteria.

– Furthermore, bacteria also produce bacteriocins, substances with antibiotic properties that inhibit the growth of undesirable bacteria such as Salmonella and Listeria monocytogenes, for example – adds the scientist.

BACTERIA IN FOOD PRODUCTION

Fermented products – made both at home and on an industrial scale – is mainly based on spontaneous fermentation, meaning that microorganisms naturally contained in fruit and vegetables are used.

The dairy industry, on the other hand, uses appropriately selected strains of lactic acid bacteria as well as yeasts and moulds. – There is a whole spectrum of these so-called starter cultures. We have separate types for the production of yoghurt, drinking yoghurt, kefir, buttermilk, cottage cheese, mould cheese, ripened cheese (those with and without holes – yes, bacteria are also responsible for the holes in cheese) – points out Anna Majkowska.

Researchers are therefore constantly looking for new strains that not only have improved properties needed in the production of a particular product, but also, for instance, reproduce quickly and have additional potential, e.g. as antibacterial agents to combat particular pathogens.

LAB BACTERIA

Researchers at the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn are, on the one hand, looking for unique bacteria by isolating them from products that are as natural as possible (e.g. unprocessed cow’s milk or natural fermented products) and, on the other hand, creating new sets of bacteria whose joint action exceeds the potential of each individual (action based on symbiosis).

In the former case, once a particular strain of bacteria has been isolated, it needs to be identified, i.e. assigned to a specific genus and species. It is also necessary to check that these bacteria multiply well (without this, it is not possible to use them on a larger scale) and that they produce sufficient quantities of essential metabolites (such as lactic acid).

In contrast, the work of creating new bacterial combinations (cultures) involves selecting bacteria with the desired properties and testing their subsequent combinations. – Bacteria are like a family – some like each other, others don’t. Therefore, combining one pair results in rapid growth and another in fighting each other. So we test all possibilities, taking many factors into account – she explains.

In the search for new strains, the Microbiology Laboratory scientists place particular emphasis on those with antimicrobial properties, fighting a specific pathogen, such as Salmonella, Campylobacter or staphylococcus.

As Anna Majkowska points out, the demand for the work of food microbiologists is high. – For example, we are currently working with a company that produces health-promoting dietary supplements. We have isolated unique strains with strong antibacterial properties for them – she says.

The Microbiology Laboratory of IAR&FR PAS supervises a collection of approximately 1,000 bacterial strains.

FUN FACTS

  • It is bacteria – the main propionic fermentation bacteria – that are responsible for the holes in the cheese.
  • It is possible to pickle not only vegetables, but also fruit, e.g. apples or plums (because the base needed for the fermentation process is sugar).
  • Pickled cucumbers (and other preserves) have much more nutritional value than raw ones, and are also more easily digested and absorbed by our body.
  • Yoghurt contains only two strains of bacteria, while kefir has dozens of them!
  • The basis for the production of kefir is the so-called kefir mushrooms/grains, which is a conglomeration of yeast (they are what make the drink slightly effervescent) and dozens of species of bacteria living in symbiosis.

Learn more about our Microbiology Laboratory HERE.

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FoodEducators: teachers and scientists together on food education

Researchers are encouraging teachers to work together to promote healthy eating habits and career opportunities in the agri-food sector for young people. This is all within the framework of the international ‘Food Educators’ programme, which offers free, easily accessible and up-to-date science-based food education materials. They are useful for biology and chemistry lessons, but also for economics and business, English or any other extracurricular activities.

– Teachers play a key role in education, and for scientists they are partners in bringing the latest knowledge to the next generation. We meet their needs by offering free and attractive educational materials – says Justyna Banasiak, project coordinator on behalf of IARFR, which is programme’s ambassador in Poland.

The materials address current and relevant topics, e.g. what does healthy or sustainable eating mean, how to read and understand food labels, what is food waste and loss about.

They also introduce jobs in the agri-food sector (e.g. food sensory analysis scientist, breeder of insects as alternative protein sources, or food photographer) and food-related professions, and develop an entrepreneurial attitude in students, e.g. by developing a plan to launch their product on the market.

THEMES AND TOPICS

The core of the programme is made up of ready-to-use lesson plans in the broad field of nutrition education, adapted for students aged 6-18 (the specific age group is indicated in each scenario). They are based on a model of active student involvement – combining elements of teamwork, role play, communication, experimentation and student-led discussion.

The scenarios are grouped into four themes: Food and health, Food and sustainability, Food science and the food system, Jobs and careers in the agri-food sector.

There are also plenty of experiments in the materials – with apples, which addresses the browning of fresh fruit and vegetables and the associated issue of food waste, or with yeast fermentation, where students learn about micro-organisms using bread as an everyday example. There are also lessons encouraging them to create a sustainable pasta recipe and design their own plant-based milk alternative.

Each lesson scenario includes additional materials, e.g. a worksheet for the student, a presentation or a worksheet for the teacher with additional tips for conducting the lesson. The scenarios also contain additional materials, including materials developed by our Institute’s scientists: podcasts, infographics, videos, or external articles from reliable and verified sources.

All these materials can be downloaded free of charge – just register HERE.

After implementing lessons based on the scenarios, teachers are expected to complete a short questionnaire, which will allow us to update and improve the materials – Justyna Banasiak points out. Every teacher who gets involved in our project will receive a certificate of participation in the international Food Educators project.

CELEBRATE WORLD FOOD DAY WITH US!

An opportunity to use the materials offered in the FoodEducators programme could be World Food Day, which is celebrated on 16 October.

Activity Weeks are taking place from 14-26 October. – We are proposing three different activities: creating a cookbook based on the idea of zero waste, learning how to check the credibility of online sources in the “Food influencer challenge”, and building awareness of sustainability, by organising a school picnic. You can join in all the activities or selected ones – explains Justyna Banasiak.

Download the brochure HERE.

CAREER DAYS

In addition, the FoodEducators programme organises Career Days every year, aimed at secondary school students. These are open to groups who register for the programme, download materials and complete a questionnaire.

The next Career Days will take place in December at the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn. Details coming soon!

About FoodEducators

FoodEducators is part of the EIT Food community, the largest and most dynamic food innovation network in the world, supported by the European Institute of Innovation and Technology (EIT). The programme is implemented in more than 10 European countries. In Poland, it is implemented and represented by the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn. Learn more: www.foodeducators.eu.

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Institute scientists among the top 2% of the world’s most influential researchers

Ten of our Institute’s scientists were included in this year’s list compiled by Stanford University and the scientific publisher Elsevier. The ‘World’s TOP 2% Scientists’ ranking is based on the Scopus database and presents scientists whose publications are most frequently cited by other authors worldwide.

The TOP 2% ranking assesses the scientific output of individual researchers according to a bibliometric index, taking into account criteria such as the Hirsch index, the number of citations and co-authorship, i.e. the place and role in the list of authors of a publication. The ranking, which is compiled annually, is divided into two lists. The first covers the research output over the entire period of the researcher’s career, while the second concerns the most recent year. This year’s list assessing the entire scientific output includes more than 217 000 scientists from all over the world, including 1244 from Poland. The 2023 summary list included more than 223 000 scientists from around the world, including 1305 from Poland.

Researchers from IARFR PAS (order based on the position on the list):

Ranking covering the entire scientific career:

  • Prof. Carsten Carlberg
  • Prof. dr. hab. Ryszard Amarowicz
  • Prof. dr hab. Henryk Zieliński
  • Prof. dr hab. Mariusz Piskuła
  • Prof. dr hab. Adam Zięcik
  • Dr hab. inż. Magdalena Karamać
  • Prof. dr hab. Andrzej Ciereszko
  • Prof. dr hab. Zenon Zduńczyk
  • Prof. dr hab. Jan Kotwica

Ranking for 2023:

  • Prof. dr hab. Ryszard Amarowicz
  • Prof. Carsten Carlberg
  • Dr Małgorzata Starowicz
  • Prof. Dr hab. Henryk Zieliński

Visit Elsevier Data Repository here.

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Our scientists will take part in the development of a new technology for oncology

The Virtual Research Institute has settled its third nationwide competition for research funding in the field of medical biotechnology. Two winning research projects have been selected to receive funding of around PLN 119 million. The Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences is a partner in one of the two winning consortia. Our scientists will work on a new technology for early diagnosis of cardiovascular complications accompanying oncological therapies.

The project entitled. ‘Personalised vascular endothelial diagnostics in oncology therapy; towards modern vasculo-oncology’ has received funding of almost PLN 79 million.

It is being implemented by a consortium consisting of a team of researchers representing: Jagiellonian University, the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences (PAS) in Wrocław, the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences (PAS) in Olsztyn, the Medical University of Gdańsk, the Maria Skłodowska-Curie National Institute of Oncology – National Research Institute in Gliwice, and the Warsaw University of Technology. The team’s leader is Professor Stefan Chłopicki of the Jagiellonian University.

As the researchers point out, changes observed in the vascular endothelium (i.e. the highly specialised lining of blood and lymphatic vessels) precede pathological changes in the cardiovascular system. Therefore, their diagnosis can detect early cardiovascular disorders resulting from complications of cancer therapies.

The aim of the research team is therefore to develop new electrochemical aptamer technology, using aptamers and other original methodological solutions. Aptamers are advanced receptors; the team of Dr Iwona Grabowska from the Department of Bioelectroanalytics of our Institute specialises in their application.

These are DNA or RNA sequences that have been engineered in laboratories to bind only one specific molecule – in this case, compounds indicative of vascular endothelial damage. – Aptamers have only recently been used in science, which is why there is still so much to learn. However, they are highly selective and it is already known that they have great potential for wide application – emphasises Iwona Grabowska.

The new technology will use new synthesised and optimised aptamers and an electrochemical detection platform. – The electrochemical method is characterised by high sensitivity, allowing us to detect even single molecules – the scientist points out.

The researchers argue that the new technology developed as part of the project will not only pave the way for modern vasculo-oncology (relating to blood vessels), but will also open up completely new directions for the development of diagnostics accompanying many other diseases associated with endothelial dysfunction.

The Virtual Research Institute (Polish: Wirtualny Instytut Badawczy – WIB) is an innovative research funding programme with high commercialisation potential in one of society’s key areas – medical biotechnology.

The Managing Entity of the WIB programme, which is responsible for organising and conducting competitions, is the Łukasiewicz Research Network – PORT Polski Ośrodek Rozwoju Technologii from Wrocław.

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INTERNATIONAL SCIENTIFIC SEMINAR – Prof. Stine Marie Ulven

Prof. Stine Marie Ulvern, head of Nutrition Department at the Medical Faculty of the University of Oslo and coordinator of MSCA NUTRIOME network  will be the guest of the upcoming Open Seminar series held as a part of the WELCOME2 ERA Chair project.

The seminar „Use of dietary intervention studies to understand the impact of dietary fat on human health” will take place on 11 October at 10:00 am at the Institute of Animal Reproduction and Food Research PAS (Tuwima 10 Str., Olsztyn).

Fatty acids and dietary fat plays a major role human health. In order to understand the biological mechanisms of fatty acids on human health we conduct dietary intervention studies and meal studies and combine traditional cardiovascular risk markers with gene expression profiling and comprehensive metabolic profiling. The focus on this presentation will be on the effect of replacing saturated fat with omega-6 fatty acids in CVD risk markers, and how omega-3 polyunsaturated fatty acids causes large variation in TG response after intervention.

Prof. Stine Marie Ulven

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Upcycling in the food industry: by-product potential

Upcycling is a higher form of recycling. It involves using by-products in such a way that a higher value product is obtained than the original one. An example in the food industry is the processing of fruit pomace. As scientists point out, pomace is in fact a rich source of health-promoting bioactive compounds.

29 September is International Day of Awareness of Food Loss and Waste, established by the United Nations. According to UN estimates, more than one billion tonnes of food went into the rubbish in 2022.

– The idea of upcycling is part of the trend towards a closed-loop economy, one of the goals of which is to reduce the amount of by-products produced. Therefore, upcycling is being promoted more and more loudly in the food industry. Science is also coming to the rescue – emphasizes Dr Bartosz Fotschki from the Biological Function of Food Team of our Institute.

According to estimates, approximately 400,000 tonnes of waste are produced annually in fruit and vegetable processing in Poland. These can include, among others, onion plant husks, marc, peelings from root vegetables, leafy cuttings and seeds. These products are most often used to produce compost or biogas. One trend is also to extract natural food colourings from them for use in the production of beverages, confectionery and bakery products, dairy products and ready meals.

The Polish juice industry is at the forefront of Europe. However, the production of juices or nectars produces a very large amount of pomace, i.e. residues of seeds, fruit or vegetables. It turns out that these by-products are a rich source of health-promoting ingredients.

– Such pomace is in fact a source of many valuable bioactive compounds with great health-promoting potential. Apple pomace, for example, contains a great ammount of dietary fibre, which, if properly prepared, could support the function of our intestines and serve as food for the bacteria in them. Proper stimulation of the intestinal microbiome with dietary fibre causes these micro-organisms to start secreting various metabolites and compounds beneficial to our health – says the scientist.

And how can such pomace be used? – For example, they can be dried and then ground and added as an ingredient to bars or muesli. On the other hand, if you separate the seeds from the pomace, you can press oil from it. Our team has repeatedly tested oils from e.g. raspberry, strawberry and blackcurrant seeds and found that they are all extremely rich in nutritionally valuable fatty acids and other bioactive compounds. Producing them from fruit pomace could be another valuable direction for the use of this by-product, according to Bartosz Fotschki.

This also fits in with the growing trend of functional foods, i.e. value-added foods with extra health benefits. In addition, fruit oils from, for example, raspberry seeds are also used in cosmetology.

In the upcycling of pomace, the Polish food industry is only taking its first steps. – However, we see a regular increase in interest in this topic, also in research project grants awarded. Certainly, it is mainly entrepreneurs who have to be willing to get involved and invest, but I think the potential is very high – the researcher points out.

So why bet on upcycling? – Using pomace as an example, we can see that with relatively little effort we can reduce the amount of waste and use its huge health-promoting potential – he concludes.

Currently, Dr Bartosz Fotschki is heading the scientific part of a research and implementation project for the development and implementation of innovative functional foods aimed at the prevention of diet-related diseases. The project is funded by the National Centre for Research and Development as part of the Nutritech: nutrition in the light of the challenges of improving societal wellbeing and climate change.

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9th Weekend with Polish Konik horses [report]

The annual horse breeders’ festival is gaining popularity among both Polish Konik experts and fans of nature and Polish heritage. This year’s 9th edition was held on 6-7 September. The event was hosted by the Research Station of the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Popielno. – We are delighted that every year the Weekend with Polish Konik gains more and more supporters – pointed out Dr Mamadou Bah, head of the station, during the opening ceremony.

Participants in the event were welcomed by Dr Mamadou Bah and Aleksandra Szymborska, who looks after the breeding of the Polish Konik horses in Popielno – a native breed derived from wild horses, the tarpan. During the opening ceremony, the importance of the event was emphasised, which is becoming more and more popular with the public every year. The prestige of the ‘Weekend with Polish Konik’ is also evidenced by this year’s guests, including representatives of horse breeders’ associations, members of the Polish Academy of Sciences, representatives of scientific and academic institutions, etc.

The first day featured the performance trials of Polish horses, for which a total of 19 horses were entered. Performance trials are ‘examinations’ developed for a specific breed of horse. Their purpose is to assess the performance requirements adopted for a given breed. The Commission pays attention to, among other things, the horse’s behaviour, speed or physical parameters evaluated at the performance test. A horse from the research station in Popielno also entered the performance test. Eventually, 15 horses completed the trials with positive results. In harness trials, 2 mares and 1 stallion were successful. In the riding trials, 12 mares took part. The horses with the best results were awarded cups.

In the harness trials, special attention was drawn to the stallion Grosz P, who finished with an excellent score of 34 points. The stallion, owned and bred by the research station in Popielno, was awarded the cup for the best performed basic field harness trial. The preparation of the horse and the driving of the carriage were handled by an employee of our station, Jacek Lipka.

The judging committee, appointed by the Polish Horse Breeders Association (PZHK) in Warsaw, consisted of Adam Domżała, director of the Warmia and Mazury Horse Breeders Association, Jan L. Slomiany, chairman of the Polish Horse Stud Book Commission, Prof. Zbigniew Jaworski and Patrycja Wojciechowska from the PZHK Stud Book and Identification Department. After the end of the trials, the commission also carried out the licensing and verification of the horses. As a result of the bonus assessment (i.e. the evaluation of the animal’s breeding and performance value), the stallion Gambit P from the breeding of the research station in Popielno scored 81 points. It is worth noting that horses receiving more than 80 points are considered to be of exceptional breeding value. On the same day, two mares entering the bonus verification were assessed. The mare Wiechlina finished the assessment with a score of 78 points, and the mare Dawka received 79 points, which shows a high level of breeding.

Foals Assessment

On the second day of the event, the presentation and evaluation of foals took place. There were 15 foals on the entry list, 9 of which came from the breeding of the Research Station of the Polish Academy of Sciences in Popielno and 6 belonged to private breeders. The committee composed of: Adam Domżała, Prof. Marta Siemieniuch, an expert in the physiology and pathology of animal reproduction and horse breeding and use, and Marek Niewiński, director of the Provincial Horse Breeders’ Association in Białystok, conducted the premiuming of the young horses.

The highest scored foal was Weksel P, the offspring of the stallion Batial P and the mare Wiechlina, bred by the IARFR PAS research station in Popielno. The stallion Gwiazdor P, also a son of the stallion Batial P, also deserves an honourable mention and was awarded second place. Third place went to the mare Legalna M from the breeding of Sławomir Niedbalski. It is worth noting that this year the mare Lotka M from the breeding of Sławomir Niedbalski placed her progeny on the podium for the third time in a row, which confirms the continuation of the high breeding level of this line.

Our sincere congratulations to all breeders, riders and drivers. Thanks and appreciation also go to all those involved in the preparation of the horses for the trials and judging.

Photo Michał Janiak

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