Science for Society

The increased popularity of the ketogenic diet is not followed by scientific knowledge on the safety of its use, points out Dr Natalia Drabińska from the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, who studies the effects of the ketogenic diet on metabolism, inflammation, selected nutritional parameters and oxidative stress in overweight and obese women.

The ketogenic diet is a high fat, low carbohydrate diet that involves putting the body into a state of ketosis. – It is quite a demanding diet, but one that gives quick results and allows you to lose unwanted kilograms in a short period of time. This is why it has become so popular in recent years – emphasises Dr Natalia Drabińska from the Department of Chemistry and Biodynamics of Food at the IAR&FR PAS in Olsztyn.

However, this does not mean that the ketogenic diet is scientifically well understood; there is still a lot of information published on the internet about the effects of this diet on the human body that is not supported by scientific research. – Although the available scientific literature indicates that the use of the ketogenic diet may be beneficial in reducing body weight, comprehensive studies guaranteeing the safety of its use are not available – she adds.

Seeking to fill this gap, the researcher, as part of an NCN-funded project, is investigating how the ketogenic diet affects metabolism, inflammation, selected nutritional parameters and oxidative stress in overweight and obese women.

The first correlation studied is the effect of the ketogenic diet on metabolism, i.e. the total biochemical processes in the body that process the energy of nutrients from food.

The next step will be to investigate the effect of the ketogenic diet on inflammation – whether and how it can reduce it.

In the effects of the ketogenic diet on selected nutritional parameters, the researcher will examine the levels of fat-soluble vitamins, namely vitamins A, D, E and K; determine the amino acid profile and the fatty acid profile.

The effect of the ketogenic diet on oxidative stress, on the other hand, will be determined by the level of antioxidant activity – what from the diet increases it and how the body responds to it; the level of enzymes involved in neutralising free radicals; and the level of lipid and nucleic acid peroxidation products. – My previous animal research showed that the ketogenic diet, even in a fairly extreme form, did not induce oxidative stress, and in fact lowered it – says the researcher.

– We have now carried out most of the research and have begun to analyse the results. Thanks to them, we will be able to answer questions asked by many people struggling with obesity and overweight, wondering whether the ketogenic diet is a safe and effective way to lose extra kilograms – emphasises Natalia Drabińska.

The cohort included 80 women (72 completed) – healthy, slightly overweight (with a BMI of 25.5-35), with an average age of 35-40 years; they were volunteers from Olsztyn and the surrounding area. For 8 weeks, women received daily balanced meals, provided by a diet catering service, and attended regular check-ups. – All the ladies are satisfied with the results of this diet, as they have shed a total of 380 kg! – indicated the researcher, who also went on a diet.

THE KETOGENIC DIET – WHAT YOU SHOULD KNOW?

The ketogenic diet involves balancing meals in such way that the main part of the calories comes from fats and the consumption of carbohydrates is maximally limited. Interestingly, the ketogenic diet was originally used to treat patients with drug-resistant epilepsy.

After four to six weeks of adaptation to such a diet, a state of ketosis occurs in the body, i.e. the body 'switches’ so that it derives energy from ketone bodies (i.e. fat accumulated in the tissues) and not – as before – from sugars (glucose).

– Before this happens, however, you need patience and determination, because the ketogenic diet can make you feel worse in the first few weeks. This is because when your body runs out of glucose and is not yet able to use ketone bodies, you will lose strength and may experience flu-like symptoms. This is when it is especially important to remember to drink plenty of water and to keep an eye on electrolyte levels. As a start, I also recommend supplementation with MCT oil – this is an oil containing medium chain acids that go directly to the liver (without passing through the pancreas) and are converted into ketone bodies there, says the researcher.

The scientist reminds us that in following any diet, it is important to proceed with caution and awareness. – In some situations, it is necessary to consult a doctor, as a high-fat diet should not be followed by people with kidney or liver problems, for example. We must remember that it is a diet based mainly on fat, which is metabolised by the liver. Therefore, loading it with a high-fat diet in people who are ill can be dangerous to their health. It is therefore worth doing a liver panel (ALT, AST, GGTP) before starting the diet, as well as checking kidney parameters such as uric acid, the level of which increases especially at the beginning of a ketogenic diet – she points out.

The ketogenic diet is an elimination diet, so it is important to ensure that it is varied and balanced. – The ketogenic diet is not about meat dripping with fat, but, above all, about healthy fat from, for example, eggs, avocados, nuts, olive oil or selected fruit and vegetables,” emphasises Natalia Drabińska.

As the researcher points out, according to current scientific knowledge, there are no contraindications to prolonged/longed use of the ketogenic diet.

Dr Natalia Drabińska’s research is being conducted as part of a project entitled 'KETO-MINOX: Effects of an isocaloric, reducing ketogenic diet on metabolism, inflammation, selected nutritional parameters and oxidative stress in overweight and obese women’, funded by the National Science Centre (NCN). The project will last until 2025.

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All you want to know about food packaging.

What the symbols on packaging mean, whether food packaging needs to be washed before throwing it away to make it recyclable and whether fresh fruit and vegetables need to be packaged – the answers to these questions are not obvious to consumers.

The European education campaign for responsible handling of food packaging, in which the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn participates, comes to help.

The EIT Food project 'InformPack’ is an international initiative of scientific and expert institutions from Denmark, the UK, Poland, Finland and Spain.

– The premise of the project is to investigate consumer behaviour, knowledge and attitudes towards food packaging. In the survey, we asked consumers, among other things, whether they pay attention to the material from which the packaging is made when buying food, whether they are able to resign from buying a product because of an excessive amount of plastic in the packaging or whether they find it difficult to segregate used packaging. On this basis, we develop educational materials to encourage consumers to behave responsibly with food packaging, both at home and outside – says Iwona Kieda of IAR&FR PAS in Olsztyn.

Analysis of the results so far has identified three key themes that consumers find most troubling and difficult. These are: lack of understanding of packaging symbols, misunderstandings about the need to clean food packaging before discarding and further recycling, over-packaging of fresh fruit and vegetables and handling of multi-material packaging.

On this basis, experts and researchers from the institutions involved in the project created educational campaigns with material in the form of infographics and animated videos.

Among other things, the materials provide information on whether food packaging should be washed, why manufacturers package fresh fruit and vegetables, what to do with multi-material juice packaging, where to dispose of bread packaging, what the triangles on packaging mean, which plastic packaging is most often recycled and the difference between compostable and biodegradable packaging

Materials on bioplastics and a campaign specifically aimed at children on good practice in the context of handling food packaging will also soon be available.

InformPack Infographics ca be found here.

InformPack Videos can be found here.

The InformPack project has also created a quiz (in adult and children’s versions) to test your knowledge. The quiz is available here.

Currently, project partners are disseminating the developed information on the Internet or in workshops in schools. In the process, they are measuring long-term changes in consumer behaviour.

– Next year, we plan to conduct consumer research in France, Italy, Germany, Denmark and Finland. In addition, in the coming months, we will continue to work on new materials, and we will also continue to hold workshops for schoolchildren and organise educational activities during science popularisation events in Poland – announces Dr Joanna Fotschki of IAR&FR PAS in Olsztyn.

EIT Food InformPack

The EIT Food InformPack project is an international initiative of scientific institutions from the University of Aarhus (Denmark), the University of Reading (UK), the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences (Poland), the VTT research network (Finland), manufacturers: Bioazul (Spain) and Maspex Group (Poland), and the Spanish supermarket chain Eroski.

The aim of the project’s activities is first and foremost to change consumer behaviour so that they deal with food packaging in a responsible and sustainable manner. It is also important to involve and raise awareness among food producers themselves, as well as local authorities, who are responsible, among other things, for the availability of recycling bins in the streets.

InformPack is funded by the EIT Food, the Knowledge and Innovation Community for Food of the European Institute of Innovation and Technology (EIT), a body of the European Union, under the EU’s Horizon 2020 framework programme for research and innovation.

For more information and free materials, visit: https://pan.olsztyn.pl/science-for-society/informpack/.

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The early stage of insulin resistance development is associated with the structures surrounding the skeletal muscles – show scientists from the Department of Prophylaxis of Metabolic Diseases at IARFR PAS.

The results of their research may be a step towards the search for a new drug for diseases associated with insulin resistance, which would have a targeted effect. 

Insulin resistance is the reduced sensitivity of tissues to insulin (a hormone that regulates blood glucose levels). Its development can lead to type 2 diabetes, cardiovascular diseases, and many other diseases.

The most important tissue related to the action of insulin are skeletal muscles, which are responsible for about 80-85% of insulin-dependent glucose uptake. These tissues, along with the structures surrounding them, were examined by scientists from the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn (their review paper on this subject can be read here).

– Our research shows that the extracellular matrix of skeletal muscle may be important in the development of insulin resistance – it is a kind of mixture produced by cells filling the free spaces between them. It includes e.g. integrins, i.e. protein receptors that transmit information between the external environment and the inside of cells. We have shown that they can be involved in the modulation of insulin action even at the early stages of insulin resistance development – explains Róża Aleksandrowicz, technologist from the Department of Prophylaxis of Metabolic Diseases IARFR PAS.

The results of research conducted together with Prof. Marek Strączkowski and Dr. Magdalena Stefanowicz, have just been published in the „Endocrine Journal” .

As the researcher explains, the role of factors related to the extracellular matrix in the development of insulin resistance is still not fully understood.

– The obtained results are the basis for further research on the role of integrins, which can help in better understanding the pathogenesis of insulin resistance, and in the future can be used to search for new drugs targeted specifically at the action of these integrins – points out Róża Aleksandrowicz.

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Raspberries contain high levels of antioxidants, which help protect the human body against many diseases, including cancer. Research by Bartosz Fotschki, PhD, from the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, has shown that in order to boost the pro-health effect of antioxidants from raspberries, combining them with prebiotics is recommended.

– Appropriate stimulation of intestinal bacteria, through prebiotic supplementation, increases the efficiency of the breakdown of raspberry polyphenols into chemical compounds with greater pro-health potential, e.g. with a beneficial effect on fat metabolism in the liver – emphasises study author Bartosz Fotschki, PhD, from the Department of Biological Function of Food of the IARFR PAS in Olsztyn.

A FEW WORDS ABOUT RASPBERRIES

Raspberries are one of the most popular fruit. They owe their success not only to their sweet, juicy taste, but also to their well-known pro-health properties.

As Bartosz Fotschki reminds us, these fruits are a rich source of bioactive compounds with strong pro-health potential, e.g. characterised by a high content of antioxidants, mainly phenolic compounds, e.g. cyanidins, anthocyanins, elagotannins and phenolic acids. Antioxidants are natural substances that can help protect the human body against the development of many diet-related diseases.

– In addition to their strong antioxidant properties, the polyphenolic compounds found in raspberries also exhibit other beneficial biological activities, including regulating inflammation, lipid metabolism, bile acid synthesis in the liver and the activity of the microbiota in the gastrointestinal tract – indicates the scientist, who is involved in exploring the pro-health properties of biologically active compounds towards the prevention and alleviation of diet-induced metabolic disorders (e.g. obesity, non-alcoholic fatty liver disease).

BOOST THE EFFECT

The pro-health effects of raspberries can be further enhanced, which is why Bartosz Fotschki examined how to influence the gastrointestinal bacteria to do more intensive 'work’ in breaking down the polyphenols from raspberries.

In his research, the scientist relied on the combination of a raspberry polyphenol preparation with the prebiotic effect of fructooligosaccharides (these are fibre products that support the growth of probiotic bacteria, which are essential for proper intestinal function).

– The results of the study confirmed an enhancement in the efficiency of metabolising polyphenols to chemical compounds with greater pro-health potential. The mechanism of action of this mixture combines an increase in the number of bacteria showing the ability to metabolise polyphenols in the gastrointestinal tract with an increased concentration of metabolites that reach the liver and regulate mechanisms related to lipid metabolism, oxidative stress and inflammation – the scientist points out.

The results of the research have been published in one of the leading journals in the discipline of food technology and nutrition: Food Research International.

In other words – by combining the consumption of raspberries with a prebiotic supplement, we boost the pro-health effects of the fruit, and this has a beneficial effect on liver metabolism, among other things.

– Further human studies are still needed, but the combination of polyphenols found in various products (e.g. juice, mousse, freeze-dried) with fructooligosaccharides could be a valuable dietary supplement to support health prevention and could already appear in many products with pro-health potential – concludes Dr. Fotschki.

The study was carried out as part of the project 'Raspberry polyphenols and their metabolites as regulatory factors in the mechanisms of non-alcoholic fatty liver disease’ (UMO-2018/31/D/NZ9/02196), funded by the National Science Centre.

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Scientists at the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn are investigating the mechanism of long-term infection of the fetus with cytomegalovirus. The virus is usually harmless for healthy people. The problem arises in immunocompromised patients and pregnant women, as it crosses the placenta and can cause serious congenital diseases.

– We are investigating the molecular basis for establishing long-term cytomegalovirus infection in neural cells. We hope to find out the factors responsible for the persistence of the virus in neural cells, and thus identify the causes of the observed damage to the nervous system associated with cytomegalovirus-induced congenital disease – emphasises Dr Magdalena Weidner-Glunde, leader of the research project and head of the Molecular Microbiology and Virology Laboratory of the IARFR PAS in Olsztyn.

THE DORMANT VIRUS

Human cytomegalovirus (HCMV) infection is very common, with more than 80 % of the Polish population infected. The primary infection in healthy people is usually asymptomatic or shows the symptoms of a slight cold, but in immunocompromised patients, e.g. after transplantation (when immunity is deliberately lowered to prevent transplant rejection) or those with AIDS, it can cause serious illness.

– HCMV is also a cause of congenital disease, as the virus is able to pass from the bloodstream of the infected mother through the placenta to the fetus. The disease resulting from congenital cytomegalovirus infection is associated with infection of the central nervous system and consequent sensory-nervous damage. Symptoms associated with HCMV congenital disease can include hearing loss, visual impairment or even intellectual disability, explains the researcher.

The herpesviruses (such as herpes virus and chickenpox virus), once they enter our body, remain in our body for the rest of our lives. The response of our immune system to their presence – i.e.  antibodies – can be detected by laboratory blood tests. They also allow us to determine whether a person has an active or latent infection.

In fact, the life cycle of herpesviruses comprises two phases: a latent (dormant) phase, from which the virus occasionally 'wakes up’ to enter the active (lytic) phase. – In the lytic phase, the virus actively multiplies by producing a large number of new viral particles. In contrast, in latency, viral protein synthesis is reduced to a minimum and there is no production of new viral particles, which prevents our immune system from effectively detecting the infection – explains Magdalena Weidner-Glunde.

HCMV has a circular genome (episome) that, during latent infection, has the ability to attach to host chromosomes and thus ensure the persistence of infection. – In other herpesviruses, it is known which protein is responsible for this binding of the viral genome to chromosomes. In the case of cytomegalovirus, we do not yet know this. In our research, we are investigating whether the viral protein IE1 is involved in the binding of the genome to the chromosomes and thus – determines the survival of the virus. Analysing the function of this protein will allow us to learn and understand how it is possible for the cytomegalovirus genome to survive in the cell for such a long time – points out Magdalena Weidner-Glunde.

LEARN THE DIFFERENCES IN CELL TYPES

Cytomegalovirus particles have different properties in terms of infection and multiplication in different cell types. So far, cytomegalovirus has been studied in its latent phase mainly in haematopoietic stem cells (from which, for example, red blood cells can arise). Recently, it has been shown that long-term HCMV infection can also take place in neural precursor cells (from which, for example, neurons are later formed). Long-term infection in these cells may be responsible for the sensory-neural damage that is symptomatic of HCMV-induced congenital disease.

Scientists from Olsztyn are also looking into comparing the mechanism of long-term virus infection in the two cell types in order to understand the differences and how cytomegalovirus disrupts the functioning of different cell types.

– For the active phase of virus infection, drugs are given to inhibit viral replication. However, there are still no drugs for the latent phase to help simply get rid of it. The results of our study will therefore be able to contribute not only to the understanding of the pathogenesis of congenital cytomegalovirus infections, but also to help develop new therapies – concludes the scientist. The research – led by Magdalena Weidner-Glunde, PhD – is being conducted as part of a project entitled “Exploring mechanisms of congenital human cytomegalovirus infection: replication, spread and latency establishment”, funded by more than PLN 3 million from the National Science Centre. The project is expected to end in May 2024.

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