Equipment and applied methods PoMD

Equipment

1. NanoDrop 2000 – Micro-Volume UV-Vis Spectrophotometer for Nucleic Acid and Protein Quantitation
2. Centrifuge Eppendorf 5430R Eppendorf
3. Thermo Stat Plus Eppendorf
4. BioScan 920-2 Maltron – body composition analyzer
5. US AutoFlow  CO2 Water-Jacketed Incubator NU-4950E
6. CellGard ES NU-480 Class II, Type A2 Biosafety Cabinet
7. OLYMPUS  IX51 inverted microscope combinations of fluorescence microscopy with additional contrast-enhancing techniques using both phase contrast and differential interference contrast (DIC) methods
8. Laboratory water bath with agitation B5-11
9. Trans-Blot Turbo Transfer System, high-performance western blotting transfer system designed to provide rapid transfers with high efficiency
10. Mini-PROTEAN Tetra cell for 1-D vertical gel electrophoresis, make running protein or DNA on polyacrylamide gels

Methods applied

1. Euglycemic hyperinsulinemic clamp
2. Indirect calorimetry
3. Assessment of body composition with bioelectric impedance analysis
4. Measurement of serum concentrations of selected proteins with ELISA
5. Biopsy of vastus lateralis muscle
6. Biopsy of subcutaneous abdominal adipose tissue
7. Isolation of peripheral blond mononuclear cells
8. Analysis of tissue gene expression with RT-PCR
9. Analysis of tissue protein content with Western blot
10. Human muscle cell cultures