The research team’s publication led by Radosław Kowalski, Ph.D., from the Department of Gamete and Embryo Biology of our Institute was among the most cited articles published from 2018 to 2022 in Theriogenology.

The review paper „Sperm quality in fish: Determinants and affecting factors” by dr Radosław Kowalski and dr Beata Cejko, describes the latest diagnostic developments in the study of fish sperm quality taking into account interspecies differences, indicating the direction of their development. The paper critically describes the principle of Computer Assisted Semen Analysis (CASA) and explains, based on our own and other researchers’ results, why there are significant discrepancies in this analysis.

The authors pointed out the critical role of the FPS (frames per second) of the camera used in the CASA study in obtaining reliable sperm motility data. In addition, a visualization of the error rule in CASA analysis when the FPS value is too low is presented for the first time.

This publication is a summary of the knowledge gained over the last 20 years by the Molecular Andrology Team. It confronts the results obtained by our team with the works of other researchers, systematizing the knowledge on the factors that affect the quality of fish spermatozoa. The conclusions included in the paper, as well as further questions facing researchers dealing with fish reproduction, constitute an important scientific value of the publication, making it useful for anyone who starts their scientific work with fish gametes,” said Radosław Kowalski, Ph.D., lead author of the publication.

The full list of most cited publications is at this link.