The Lab of Dr Antonio Galvao is recruiting PhD student to undertake in vitro/in vivo work in ovarian function in mouse models for maternal obesity. The escalating epidemic of overweight and obesity is a major burden for our health systems. Obesity leads to long-term health problems, such as diabetes, cardiovascular disease or infertility. In the lab we use mouse models and/or in vitro systems of ovarian/follicular culture to study the impact of maternal obesity in ovarian function and oocyte biology. The project investigates particularly the impact of altered leptin signalling in the ovary, a major feature of obese mothers, on the establishment of oocyte epigenome and metabolic regulations in both gamete and surrounding granulosa cells throughout folliculogenesis. The present research programme benefits from the close collaboration with the Laboratory of Dr Gavin Kelsey, Babraham Institute, Cambridge, UK, a world leader in single cell transcriptome and methylome analysis. The appointed student will be responsible for leading his/her own research projects and mainly working closely with other members of the team. The job holder will use mouse and cell based experimental systems to carry out transcriptome, methylome and lipidome analysis. Studies may extend to engineered cell lines to validate new findings. Training in multiomic analysis will be provided through the present network.
This work will lead to important advances in our understanding of the impact of altered leptin signalling in oocyte epigenome establishment, profiling also metabolic and gene expression changes in granulosa cells and other underlying mechanisms.
Person specification (desired skills):
- MSc in molecular biology, cell signalling or related biological discipline,
- Basic knowledge on cell signalling studies (desired),
- Previous experience in mammalian cell culture (desired),
- Previous experience in gene expression analysis (qPCR, WB, IF & IHC),
- Able to understand and communicate in the English to a level appropriate for the position,
- Ability to work independently and as part of a team,
- Excellent organisational skills, with good time management,
- Excellent interpersonal skills with the ability to communicate with staff at all levels.
Conditions:
- Maximum scholarship duration: 4 years,
- Type of position: PhD scholarship,
- Position: doctoral student,
- PhD scholarship: 4 276 PLN per month (~3700 PLN net salary).
Documents to present with the application:
- Cover letter highlighting the suitability of the candidate to the post (max 2 pages),
- CV (max 6 pages),
- One contact reference (one of letters must be from the last supervisor/line manager).
Applications will be accepted until 15st of October 2021 and interviews will be held soon after. For informal questions about the post please contact Dr Galvao (a.galvao@pan.olsztyn.pl).
For more information, please consult: www.pan.olsztyn.pl.
