Semen Analysis and Cryopreservation Zone:
- flow cytometers (Muse, Guava, and FlowSight) – devices used for sperm quality analysis, including the assessment of viability, membrane fluidity, mitochondrial potential, and the presence of reactive oxygen species (ROS). In addition, they enable the characterization of extracellular vesicles,
- CASA system (Computer Assisted Sperm Analysis) – a computer-based system enabling precise measurement of sperm motility parameters, used, among others, to assess the impact of storage and cryopreservation procedures on semen quality,
- NucleoCounter – enabling rapid and accurate measurement of sperm concentration and the proportion of live and dead cells,
- micro-osmometer – a device for measuring the osmolality of fluids, both biological (semen plasma, blood plasma) and chemical,
- IceCube 4s system – an advanced system for programmable, computer-controlled semen cryopreservation,
- Easy Coder printer – a device for applying identification data (e.g. species names, individual IDs, dates) to straws, ensuring full traceability of samples,
- MPP Quattro automatic machine – a system for filling and sealing cryopreservation straws,
- straw sealer – devices for manual sealing of straws, used as an alternative to the MPP Quattro system.
Oocyte Analysis and In Vitro Embryo Culture Zone
- Zeiss inverted microscope with fluorescence system and micromanipulators, used for survival analysis of oocytes and embryos, with dedicated MicroImage software enabling documentation and analysis of morphological parameters,
- Zeiss stereomicroscope for the selection, classification, and isolation of oocytes,
- Olympus CKX41 inverted microscope for the assessment of quality and developmental stage of gametes and embryos,
- laminar flow cabinets and tri-gas incubators (CO₂, O₂, N₂) enabling long-term culture of mammalian embryos under precisely controlled environmental conditions,
- system for aspiration of oocytes from ovarian follicles,
- Primo Vision digital real-time embryo development monitoring system, enabling research on embryogenesis and the dynamics of cell divisions,
- a set of tools for in-house production of microcapillaries, including a puller, microforge, and microgrinder – essential for micromanipulation procedures such as ICSI, nuclear transfer, or microinjection of genetic material.
