Our research is focused on the pathogenesis of insulin resistance In subjects at risk of developing type 2 diabetes. The study groups include subjects with positive family history of type 2 diabetes and subjects with overweight or obesity. Lean healthy subjects without family history of diabetes serve as a control group.
We utilize methods for in vivo measurement of insulin sensitivity and glucose and lipid oxidation. They are combined with the precise assessment of body composition, with a special focus on visceral fat content. We perform isolation of peripheral blood mononuclear cells and the biopsies of subcutaneous abdominal adipose tissue and vastus lateralis muscle, with a subsequent analysis of tissue gene expression with RT-PCR.
We conduct interventional studies, with the aim to assess the effect of the combination of low-calorie diet with the selected diet supplements on visceral adipose tissue content and insulin sensitivity in obese subjects with normal glucose tolerance.