Skip to main content

Equipment and applied methods C&BoF

Institute of Animal Reproduction and Food Research of PAS > Research & Dissemination > Scientific Groups > Chemistry and Biodynamics of Food > Equipment and applied methods C&BoF

Equipment:

  1. Micro-UHPLC gradient system with autosampler (LC200, Eksigent) coupled with TripleTOF 5600+ mass spectrometer (AB SCIEX, USA);
  2. Micro-UHPLC gradient system with autosampler (LC200, Eksigent) coupled with QTRAP 5500 mass spectrometer (AB SCIEX, USA);
  3. HPLC gradient system with autosampler (LC-20) coupled with SPD-M20A DAD detector (Shimadzu, Japan);
  4. HPLC gradient system with autosampler (LPG-3400M, WPS-3000TSL, Dionex, USA) coupled with multi-channel electrochemical detector (5600A, ESA, USA) and fluorescence detector (FLD-3400RS, Dionex, USA);
  5. Software for pharmacokinetic calculation Biokinetica;
  6. HPLC gradient system with autosampler (LC-10, Shimadzu, Japan) coupled with DAD detector (SPD-M10AVP, Shimadzu, Japan) and mass spectrometer (LCMS-QP8000a, Shimadzu, Japan);
  7. HPLC gradient system with autosampler (LC-10, Shimadzu, Japan) coupled with UV-Vis detector (SPD-10A, Shimadzu, Japan) and fluorescent detector (RF-10AXL, Shimadzu, Japan);
  8. HPLC isocratic system (LC-10, Shimadzu, Japan) coupled with two amperometric detectors (ICA-3000, TOA, Japan and ED40, Dionex, USA) and refraction index detector (RID-6A, Shimadzu, Japan);
  9. Gas chromatograph (7890A, Agilent, USA) equipped with MS and FID detectors;
  10. UV-VIS spectrophotometer with temperature-controlled measuring cell (UV-1601PC, Shimadzu, Japan);
  11. Luminescent spectrophotometer (LS 50 B, Perkin Elmer, USA);
  12. Photochem analyzer (Analytical Jena, Germany);
  13. Modular oven (DC-21, Sveba-Dahlen, Sweden);
  14. UV-VIS spectrophotometer (UVD-2950, LABOMED,INC., USA);
  15. POLARTRONIC E polarimeter (SCHMIDT+HAENSCH, Germany).

Research methods:

  • determination of the level of metabolites of phytocompounds in biological fluids and tissues of organisms;
  • analysis of metabolic biomarkers of the intake of food of plant origin;
  • analysis of the bioavailability of food components and determination of pharmacokinetic parameters;
  • analysis of plant metabolome;
  • quantitative and qualitative analysis of phytocompounds:
    • anthocyanins;
    • flavonols;
    • proanthocyanidins;
    • isoflavones;
    • flavanones;
    • catechins;
    • phenolic acids;
    • oligosaccharides;
    • glucosinolates;
    • degradation products of glucosinolates;
    • tocopherols;
    • tocotrienols;
    • phytates;
    • P6 – P4 compounds;
    • fatty acids;
    • vitamin C;
    • betalains;
    • reduced (GSH) and oxidized glutathione (GSSG).
  • analysis of antioxidative properties:
    • TEAC;
    • DPPH;
    • ORACFL;
    • PRTC;
    • photochemiluminescence,
  • analysis of products of Maillard reaction advancement:
    • furosines;
    • FAST index;
    • fluorescence of intermediate products of Maillard reaction advancement;
    • browning index.
  • analysis of podophyllotoxin derivatives;
  • analyses of enzymatic availability of food products (in vitro);
  • assays of physicochemical properties of raw materials, finished products and food components, and evaluation of technological traits of experimental bakery products;
  • analysis of starch, saccharides, amylase-resistant starch and dietary fibre, and protein analysis with the method of electrophoretic separation on polyacrylamide gels;
  • measurements of rheological properties of hydrocolloids, viscoelastic or pseudoelastic substances, and non-Newtonian fluids;
  • measurements of two-phase systems (foaming and emulsifying properties);
  • analysis of aerating properties; generation of emulsion systems including, among others, mixtures of bile acids in analyses of sorption capacity against bile acids.