Research methods and equipment
Cell and molecular biology methods:
- Isolation and culture of primary cells,
- Work with cell lines:
- culture of adherent and suspension cells,
- transfection of cells,
- lentiviral transduction of cells,
- Isolation of DNA and RNA from tissues, cultured cells and body fluids,
- Gene expression analysis by real-time RT PCR,
- Analysis of gene polymorphisms by DNA sequencing,
- Western-blot analysis of protein expression;,
- FACS,
- Detection of specific DNA fragments using Southern blot hybridization.
Equipment:
- HERASAFE KS ThermoScientific laminar flow cell culture chamber,
- cell culture incubators,
- RealTime PCR Applied Biosystems Step One Plus,
- Pegasus 4D, GCxGC TOF MS,
- IN Cell Analyzer 2000HS,
- Olympus CKX41 brightfield inverted microscope,
- Tecan infinite m200pro plate reader,
- DNA/RNA and protein electrophoresis apparatus with comb kit,
- electro-transfer unit,
- electrophoresis apparatus with comb set,
- CASA system for computerised semen analysis,
- autoclave,
- ultrasonic cleaner Elma Elmasonic S 100H,
- dry sterilisation oven,
- UltraTurax homogeniser,
- ThermoScientific STR8 centrifuge with cooling and variable rotors for 1.5, 2.0, 15 and 50 ml tubes,
- laboratory centrifuge with cooling.
