Alzheimer’s disease results in a progressive loss of cognitive function and dementia affecting 36 million people, with the prevalence expected to double every 20 years. Thus, the development of methods for early diagnosis, as well as for preventions is very necessary.
We have joined to this vivid research area, working on the development of the new analytical tools suitable:
- for determination of possible biomarkers of Alzheimer’s and other neurodegenerative diseases present in human plasma (possible application – early diagnosis)
- for investigation of interactions between proteins and small organic molecules – potential drugs (possible application in the prevention of neurodegenerative diseases)
Very promising research direction in this field is connected with electrochemical biosensors based on bi-functional redox active layer immobilized on the electrode surface. Such layers could serve as a transducer as well as an analytically active element. The redox centers, located inside the active sensing layers, are responsible for transduction of signals coming from recognition process to the analytical readable signals.
The features of redox active SAMs are mostly influenced by the distance between the redox centre and electrode, structure of bridge connecting the center and electrode, as well as the molecular environment around the redox centre.
The crucial parameter deciding about the sensitivity and selectivity of biosensors based on redox active layer containing the proteins applied as the recognition elements is their oriented immobilization on the electrode surface.
We are working on a new systems suitable for oriented immobilization of his-tagged proteins, based on thiol derivative of dipyrromethene–Cu(II) complex deposited on gold electrode surface and other related compounds.